On the structure of proteoglycan core

SynopsisPorcine submaxillary mucin (PSM) is a glycoprotein composed of a protein core and frequent, short oligosaccharide side chains. We report static and dynamic light scattering experiments and intrinsic viscosities for PSM in aqueous solvent systems. In 0.1M NaCl solution, the data suggest PSM exists as large, internally branched, highly hydrated, polydisperse aggregates that slowly dissociate to give a stable species of weight-average molecular weight (M,) 7.4 X lo6. In 6M GdnHCl solution, the noncovalent bonds between PSM molecules are broken, giving a highly elongated molecule of M , = 2.0 X lo6. The irreversible nature of this dissociation suggests that the forces that stabilize the native aggregates of PSM in 0.1M NaCl are specific in nature. On reduction of PSM with mercaptoethanol, the polydispersity decreases and M , also decreases to 9 X lo5. A discrete change is observed in the solution properties of PSM in 0.1M NaCl at a concentration of 2 mg/mL, manifested by a sudden decrease in the translational diffusion coefficient, an increase in viscosity number, and a decrease in slope of the osmotic compressibility. We tentatively propose that a weak and reversible secondary association process occurs at this concentration, although a purely hydrodynamic interaction cannot be ruled out.

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Solution properties of porcine submaxillary mucin

Shogren

Jamieson

Blackwell

et al. 1983

Biopolymers

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Deglycosylation of chondroitin sulfate proteoglycan by hydrogen fluoride in pyridine

Olson¹,

Krueger²,

Schwartz³

1985

Analytical Biochemistry

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Bovine nasal proteoglycans: study of the cyanogen bromide fragments from the protein-keratan sulphate core and the hyaluronic acid-binding region

Périn

Bonnet

Jollès

1981

International Journal of Biological Macromolecules

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On the structure of proteoglycan core

Cited by 7 publications

References 21 publications

Solution properties of porcine submaxillary mucin

Solution properties of porcine submaxillary mucin

Deglycosylation of chondroitin sulfate proteoglycan by hydrogen fluoride in pyridine

Bovine nasal proteoglycans: study of the cyanogen bromide fragments from the protein-keratan sulphate core and the hyaluronic acid-binding region

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