On the succinic acid production from xylose by growing and resting cells of Actinobacillus succinogenes: a comparison

Escanciano, Itziar A.; Ladero, Miguel; Santos, Victoria E.

doi:10.1007/s13399-022-02943-x

Cited by 7 publications

(13 citation statements)

References 47 publications

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“…This is the first work in which the operation with resting cells is studied in different types of operation, allowing us to optimise the stages of cell growth and SA production separately and reduce the operating costs associated with the nitrogen source and SA purification. Additionally, the effect on production with cells in growth and resting state of the use of a cheaper culture medium than the one used in previous studies of the research group was compared [17]. Finally, kinetic modelling of the bioprocess was developed to predict the evolution of biomass, xylose, succinic acid and the by-product concentration throughout the fermentation time.…”

Section: Glucosementioning

confidence: 99%

“…Following the procedure described in a previous study [17], thawed bacteria were incubated at 37 • C for 24 h in anaerobic bottles with TSB medium. Subsequently, a twostep procedure was developed to adapt the biocatalyst to the carbon source.…”

Section: Bacterial Strain Adaptation Preinoculum and Inoculum Stagesmentioning

confidence: 99%

“…Furthermore, the development of an effective biocatalyst is also a key factor that determines the viability of the bioprocess. Recently, A. succinogenes in the resting state was proven to be a promising biocatalyst, revealing a higher selectivity to the target acid when compared to growing cells [17]. In that previous study [17], a complex growth medium, TSB, had been selected due to its richness in nutrients, considered in the bibliography as a preferred medium to promote cell growth [22][23][24].…”

Section: Influence Of Medium Composition On Succinic Acid Production ...mentioning

confidence: 99%

“…Considering that downstream operations represent approximately 60% of the overall operating costs in the SA production process, it is essential to reduce the by-product generation during fermentation in order to design a competitive process on an industrial level. To do this, this research group carried out a previous study on the viability of succinic acid production employing a biocatalyst composed of A. succinogenes cells in a resting state [17]. Operating under nitrogen-limited conditions, and formulating a medium composed exclusively of a carbon source and a buffer solution to maintain osmotic pressure, resting cells are metabolically active even though cell growth is impeded.…”

Section: Introductionmentioning

confidence: 99%

“…Concurrently, the number of by-products is also reduced dramatically [18][19][20], this being one of the main advantages associated with this type of operation. In fact, Escanciano et al (2022) [17] managed to reduce the quantity of by-products generated by 27.5% in the production of SA from xylose. In order to obtain cells for subsequent use in the production stage in resting state, it is necessary to carry out a conventional fermentation, although it requires the optimisation of conditions so that the cells have the appropriate metabolic state for their subsequent use in resting cells.…”

Section: Introductionmentioning

confidence: 99%

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Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

et al. 2023

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Succinic acid (SA) is one of the most prominent C4 biomass-based platform chemicals that can be biologically obtained. This article verifies, for the first time, the possibility of producing succinic acid with fed-batch or repeated batch operations with Actinobacillus succinogenes in a resting state, that is, in the absence of a nitrogen source. In this work it is possible to optimise separately the stages of cell growth and production in the fed-batch or repeated batch modes, minimising the costs associated with the nitrogen source and facilitating the subsequent purification of SA. These experiments were carried out with xylose, the most abundant monosaccharide in hemicelluloses, with the results subsequently being compared to those obtained in equivalent operations carried out with cells in a state of growth. First, a cost-effective synthetic growth medium was proposed and successfully employed for SA production. Biocatalysts’ reutilisation showed that the bioprocess can be carried out successfully in repeated batch and fed-batch modes. The best mode for growing cells is repeated batch, achieving a maximum productivity of 0.77 g‧L−1‧h−1, a selectivity of 53% and a yield of 51% with respect to xylose consumed. In contrast, the fed-batch mode was found to be the most convenient mode with resting cell biocatalyst, reaching a maximum productivity of 0.83 g‧L−1‧h−1, a selectivity of 0.78 g‧g−1 and a yield of 68% with respect to the xylose consumed. In addition, by-product formation is significantly reduced when employing resting cells. An unstructured non-segregated kinetic model was developed for both biocatalysts, capable of simulating cell growth, xylose consumption, SA production and by-product generation, with successful estimation of kinetic parameters supported by statistical criteria.

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Section: Glucosementioning

confidence: 99%

Section: Bacterial Strain Adaptation Preinoculum and Inoculum Stagesmentioning

confidence: 99%

Section: Influence Of Medium Composition On Succinic Acid Production ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

et al. 2023

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Integral use of brewery wastes as carbon and nitrogen sources for the bioproduction of succinic acid

Escanciano,

Blanco,

Santos

et al. 2024

Biomass Conv. Bioref.

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Circular bioeconomy is one of the major socio-economic objectives for the twenty-first century, which includes the use of biomass waste and its transformation through environmentally friendly processes into biorefinery building blocks. Among these compounds, succinic acid (SA) obtained by fermentation stands out. This work demonstrates the feasibility of using beer bagasse and spent brewer’s yeast as carbon and nitrogen sources for the bioproduction of SA with Actinobacillus succinogenes. The use of a progressive enzymatic treatment liberated simple monosaccharides and peptides that were used by the microorganism, in a subsequent fermentation. Compared to the use of commercial xylose and yeast extract, the used of beer wastes obtained better yields (0.77 g g −1) and selectivity (76%), though with a slightly lower productivity (0.15 g L −1 h −1). Finally, an unstructured non-segregated kinetic model was successfully fitted, facilitating the future performance of bioreactor design, techno-economic analysis, scaling of the process, or design of a control system.

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Succinic acid production by Actinobacillus succinogenes using acid and enzymatic hydrolysates of potato and beer wastes and repeated batch operation

Escanciano,

Ladero,

Blanco

et al. 2024

Biomass and Bioenergy

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On the succinic acid production from xylose by growing and resting cells of Actinobacillus succinogenes: a comparison

Cited by 7 publications

References 47 publications

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

Study on the Operational Modes Using Both Growing and Resting Cells for Succinic Acid Production from Xylose Kinetic Modelling

Integral use of brewery wastes as carbon and nitrogen sources for the bioproduction of succinic acid

Succinic acid production by Actinobacillus succinogenes using acid and enzymatic hydrolysates of potato and beer wastes and repeated batch operation

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