On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion

Knöll, Bernd; Schmidt, Hannes; Andrews, William D.; Guthrie, Sarah; Pini, Adrian; Sundaresan, Vasi; Drescher, Uwe

doi:10.1242/dev.00726

Cited by 44 publications

(40 citation statements)

References 65 publications

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“…It is likely that coordinated regulation of these processes by different guidance cues gives rise to the mature glomerular maps observed in the accessory and main olfactory systems. Consistent with this notion, vomeronasal neurons of the accessory olfactory system are responsive to selective secreted semaphorins and slits in vitro (Cloutier et al, 2002;Knoll et al, 2003). Furthermore, both the secreted semaphorin receptor Npn-2 and the slit receptor Robo-2 are expressed in complementary populations of spatially segregated sensory neurons in the VNO (Cloutier et al, 2002;Knoll et al, 2003).…”

Section: Discussionmentioning

confidence: 71%

“…1 D-F ) (Marillat et al, 2002;Knoll et al, 2003). These slit and Robo expression patterns, coupled with the ability of Slit-1 to repel vomeronasal neurons in vitro (Knoll et al, 2003), suggest that Slit-1-Robo-2 signaling mediates targeting and fasciculation of basal vomeronasal projections to the posterior AOB.…”

Section: Mistargeting Of G␣ O -Expressing Vomeronasal Axons To the Anmentioning

confidence: 95%

“…Interestingly, slit proteins are expressed in both the VNO and AOB, and also in a subset of mitral cells in the MOB (Marillat et al, 2002;Knoll et al, 2003). Furthermore, the slit receptor, Robo-2, is restricted in its expression to basal vomeronasal sensory neurons (Marillat et al, 2002;Knoll et al, 2003) (Fig.…”

Section: Mistargeting Of G␣ O -Expressing Vomeronasal Axons To the Anmentioning

confidence: 98%

“…The Slit and Robo families of axon guidance molecules have been proposed to function as cues for basal vomeronasal axons (Knoll et al, 2003). Indeed, vomeronasal neurons are repelled by slit proteins in vitro, and the Slit receptor Robo-2 is expressed in vomeronasal neurons located in the basal region of the VNO (Marillat et al, 2002;Knoll et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Differential Requirements for Semaphorin 3F and Slit-1 in Axonal Targeting, Fasciculation, and Segregation of Olfactory Sensory Neuron Projections

Cloutier¹,

Sahay²,

Chang³

et al. 2004

J. Neurosci.

106

105

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The formation of precise stereotypic connections in sensory systems is critical for defining accurate internal representations of the external world; however, the molecular mechanisms underlying the formation of sensory maps are poorly understood. Here, we examine the roles of two structurally unrelated repulsive guidance cues, semaphorin 3F (Sema3F) and Slit-1, in olfactory sensory axon fasciculation, targeting, and segregation. Using sema3F Ϫ/Ϫ mice, we show that Sema3F is critical for vomeronasal sensory neuron axonal fasciculation and for segregation of these sensory afferents from the main olfactory system; however, Sema3F plays only a minor role in targeting of apical vomeronasal neuron axons to the anterior accessory olfactory bulb (AOB). In addition, we show that Sema3F is required for lamina-specific targeting of olfactory sensory axons within the main olfactory system. In contrast to Sema3F, Slit-1 is dispensable for fasciculation of basal vomeronasal neuron axons but is critical for targeting these axons to the posterior AOB. These results reveal discrete and complementary roles for secreted semaphorins and slits in axonal targeting, fasciculation, and segregation of olfactory sensory neuron projections.

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Section: Discussionmentioning

confidence: 71%

Section: Mistargeting Of G␣ O -Expressing Vomeronasal Axons To the Anmentioning

confidence: 95%

Section: Mistargeting Of G␣ O -Expressing Vomeronasal Axons To the Anmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential Requirements for Semaphorin 3F and Slit-1 in Axonal Targeting, Fasciculation, and Segregation of Olfactory Sensory Neuron Projections

Cloutier¹,

Sahay²,

Chang³

et al. 2004

J. Neurosci.

106

105

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“…The vomeronasal receptors situated in the apical epithelium of the VNO send information to the anterior AOB, while those receptors located in the basal part send their axons to the posterior AOB, that is to say, between both structures, VNO/AOB, there is a zone-to-zone projection. Recently, an elegant experiment has been published about the same issue (Knöll et al, 2003). This topographical or zonal organization was previously established between the sensory epithelium of the main olfactory system and the MOB, although in this case four main zones were defined: I, II, III, and IV (Vassar et al, 1993;Ressler et al, 1994;Yoshihara et al, 1997;Mori et al, 2000).…”

Section: Form and Topography Of Aobmentioning

confidence: 90%

General organization of the perinatal and adult accessory olfactory bulb in mice

et al. 2006

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The vomeronasal system is currently a topical issue since the dual functional specificity, vomeronasal system-pheromones, has recently been questioned. Irrespective of the tools used to put such specificity in doubt, the diversity of the anatomy of the system itself in the animal kingdom is probably of more importance than has previously been considered. It has to be pointed out that a true vomeronasal system is integrated by the vomeronasal organ, the accessory olfactory bulb, and the so-called vomeronasal amygdala. Therefore, it seems reasonable to establish the corresponding differences between a well-developed vomeronasal system and other areas of the nasal cavity in which putative olfactory receptors, perhaps present in other kinds of mammals, may be able to detect pheromones and to process them. In consequence, a solid pattern for one such system in one particular species needs to be chosen. Here we report on an analysis of the general morphological characteristics of the accessory olfactory bulb in mice, a species commonly used in the study of the vomeronasal system, during growth and in adults. Our results indicate that the critical period for the formation of this structure comprises the stages between the first and the fifth day after birth, when the stratification of the bulb, the peculiarities of each type of cell, and the final building of glomeruli are completed. In addition, our data suggest that the conventional plexiform layers of the main olfactory bulb are not present in the accessory bulb. Anat Rec Part A, 288A: 1009-1025, 2006. 2006 Key words: vomeronasal system; accessory olfactory bulb; growth; anatomy; mouseOdors are detected in the majority of mammals by means of two systems, the main olfactory system (MOS) and the vomeronasal system (VNS) (Halpern, 1987;Brennan, 2001;Halpern and Martínez-Marcos, 2003), which, despite some differences, basically share a common pattern of organization (Mori, 1987). Each consists of a sensory epithelium, two olfactory bulbs, and different telencephalic structures. This similarity results in the olfactory information being processed in a like manner in both systems, although there are also some specific morphological differences, and at molecular level different genes are involved in the transduction mechanism (Buck and Axel, 1991;Dulac and Axel, 1995;Mombaerts, 1999).The main olfactory (MOB) and the accessory olfactory bulbs (AOB), which are the first relay stations in each system, play a key role in the physiological mechanism of olfaction. Within the bulbs, glomeruli organize the corresponding input and output information

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Morphological and Immunohistochemical Features of the Vomeronasal System in Dogs

Salazar

Cifuentes

Sánchez‐Quinteiro

2012

The Anatomical Record

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Each of the structures integrating the sense of smell in mammals has a different degree of development, even in the so-called macrosmatic animals, according to the capacity of the olfactory system to detect thousands of different chemical signals. Such morphological diversity implies analogous physiological variation. The study of the accessory olfactory system, also known as the vomeronasal system, is a useful way to analyze the heterogeneity of the sense of smell. Macrodissection and microdissection methods as well as conventional histology and immunohistochemistry protocols were used to study aspects of the vomeronasal organ and the accessory olfactory bulbs in dogs. Observations regarding the end of the anterior part of the vomeronasal duct have been emphasized. Both lectins, Ulex europaeus agglutinin I and Lycopersicum esculentum agglutinin, and one G protein, G ai2 , show a similar pattern of binding in the sensory epithelium of the vomeronasal organ and in the vomeronasal nerve and glomerular layers of the accessory olfactory bulb, whereas the expression of protein G ao was not observed. Taken together, our results emphasize the contribution of comparative data to our understanding of the vomeronasal system function. Anat Rec, 296:146-155, 2013. V C 2012 Wiley Periodicals, Inc.

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On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion

Cited by 44 publications

References 65 publications

Differential Requirements for Semaphorin 3F and Slit-1 in Axonal Targeting, Fasciculation, and Segregation of Olfactory Sensory Neuron Projections

Differential Requirements for Semaphorin 3F and Slit-1 in Axonal Targeting, Fasciculation, and Segregation of Olfactory Sensory Neuron Projections

General organization of the perinatal and adult accessory olfactory bulb in mice

Morphological and Immunohistochemical Features of the Vomeronasal System in Dogs

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