Abstract-This paper discusses the effect of a gamified learning system for students of the master course on Web Design and Programming performed at the Faculty of Organization and Informatics. A new set of usability metrics was derived from web-based learning usability, user experience and instructional design literature and incorporated into the questionnaire which consists of three main categories: Usability, Educational Usability and User Experience. The main contribution of this paper is the development and validation of a questionnaire for measuring the usability of a gamified e-learning course from students' perspective. Usability practitioners can use the developed metrics with confidence when evaluating the design of a gamified e-learning course in order to improve students' engagement and motivation.