The primary objective of this chapter is to examine the AI applications for healthcare 4.0. Using a wide range of contemporary technologies, such as digitization, artificial intelligence, user response data (ergonomics), human psychology, the internet of things, machine learning, big data mining, and augmented reality, one of the great success stories of our day is healthcare. Worldwide life expectancy has increased due to the tremendous advancements in medical research. But when people live longer, healthcare systems must deal with more people needing their services, more money spent on them, and a staff that finds it more challenging to care for patients. A healthy, productive society depends heavily on the healthcare industry, making it one of the most critical industries in the larger big data environment. Artificial intelligence (AI), which builds on automation, has the potential to transform healthcare and assist in addressing some of the issues mentioned above. AI can support healthcare professionals, including physicians and nurses, in their day-to-day jobs. Artificial intelligence (AI) can improve patient outcomes by enhancing the quality of life and preventive care and producing more accurate diagnoses and treatment regimens. This book provides an overview of the most recent advancements in artificial intelligence (AI) applications in biomedicine, encompassing pharmaceutical processing, disease diagnosis, patient monitoring, biomedical information, and biomedical research. A summary of the most recent developments in the use of AI in healthcare is also provided, along with a road map for creating safe, dependable, and efficient AI systems and a discussion of potential future directions for AI-assisted healthcare systems. Numerous uses of AI exist in the medical field. Healthcare 4.0 has brought about a paradigm shift in the healthcare industry, drawing inspiration from Industry 4.0. Therefore, how the digital revolution in healthcare will affect the quality of medical care is still being determined. This study results will help the new researchers and healthcare institutions.