“…A few years later, Rosenberg (1936) cultivated T. augusta from a tree toad, Hyla regilla, in a mixture of the enteric rectal contents of the animal and an equal amount of 0.85 per cent sodium chloride, a medium first described by Kofoid and Swezy (1915b). Rosenberg (1936) also reported the effect of hydrogen-ion concentration on the viability of the organisms. According to this investigator, the change in the pH value toward the acidity caused the death of the organisms while the maximal growth was obtained with constant pH value of 7.55, the approximate pH value of the rectal con tent of the frog.…”