On Tissue Oxygen and Hypoxia

Swartz, Harold M.

doi:10.1089/ars.2007.1694

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…Standard methods for measuring pO 2 in vitro have included the Clarke‐type electrode and, more recently, the use of optical probes with immobilized fluorophores on the tip of an optical fiber. Another option is the use of oxygen sensitive fluorophores dissolved in the medium (see, e.g., Ref 5…”

Section: Introductionmentioning

confidence: 99%

In vitro cell culture pO₂ is significantly different from incubator pO₂

Bambrick

Kostov

Rao

2011

Biotechnology Progress

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Continuous noninvasive monitoring of peri-cellular liquid phase pO2 in adherent cultures is described. For neurons and astrocytes, this approach demonstrates that there is a significant difference between predicted and observed liquid phase pO2. Particularly at low gas phase pO2s, cell metabolism shifts liquid phase pO2 significantly lower than would be predicted from the O2 gas/air equilibrium coefficient, indicating that the cellular oxygen uptake rate exceeds the oxygen diffusion rate. The results demonstrate the need for direct pO2 measurements at the peri-cellular level, and question the widely adopted current practice of relying on setting the incubator gas phase level as means of controlling pericellular oxygen tension, particularly in static culture systems that are oxygen mass transfer limited.

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Section: Introductionmentioning

confidence: 99%

In vitro cell culture pO₂ is significantly different from incubator pO₂

Bambrick

Kostov

Rao

2011

Biotechnology Progress

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“…The polarographic Clark-type electrode (Clark et al (1953)) is the current standard tool for measuring partial oxygen pressure (pO 2 ) [kPa] of tissue (Cheema et al (2008);Swartz (2007); Pogue et al (2001) (Lee & Tsao (1979)). As a result, the sensitivity of Clark electrodes is directly related to the pO 2 of the environment it is measuring.…”

Section: Oxygen Quantificationmentioning

confidence: 99%

Spatial-Temporal Dose Metric Sampling for Explicit Photodynamic Therapy

Henderson¹,

Lai²,

Lilge³

2010

Data Acquisition

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“…In one study (Pettersen et al 2005), microelectrodes were used to monitor the pO2 in cell cultures under static conditions, significant differences between measured peri-cellular oxygen tension and predicted oxygen tension was found in ambient air. Another reported method is to use oxygen sensitive fluorophores dissolved in the medium for pO2 measurement (Swartz 2007), but the sensitive fluorophore may be toxic to the cell.…”

Section: Introductionmentioning

confidence: 99%

Cell culture pO ₂ monitoring with a fiber optic oxygen sensor

Liu

Wang

Zeng

et al. 2016

Biomed. Phys. Eng. Express

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Monitoring of cellular cultures oxygen partial pressure (pO2) in liquid phase with a fluorescence quenching based fibre optic oxygen sensor is reported. The sensitivity of the fibre optic oxygen sensor was evaluated in gas phase and calibrated in liquid phase before the monitoring of cell culture pO2 with a luminescence phase detection system. In the experiment of cell culture pO2 monitoring the porcine kidney (PK-15) cells were used as the cell samples and the experimental results showed that the sensor is suitable for monitoring of pO2 in gas and liquid phase. And we found there are different measured pO2 level in liquid phase for different cell densities in cell culture at ambient air environment, which has a PO2 of 21kPa corresponding to an oxygen concentration of 21%. Under the condition of low pO2 in gas phase and high cell density, the cell metabolism could shift pO2 in the liquid phase much lower than that would be from the O2 gas/air equilibrium coefficient, which indicates that the cellular oxygen uptake rate exceeds the oxygen diffusion rate. Therefore there is a need for direct pO2 monitoring in cell culture, particularly in static high density cell culture systems, in which oxygen mass transfer is limited.

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On Tissue Oxygen and Hypoxia

Cited by 4 publications

References 12 publications

In vitro cell culture pO₂ is significantly different from incubator pO₂

In vitro cell culture pO₂ is significantly different from incubator pO₂

Spatial-Temporal Dose Metric Sampling for Explicit Photodynamic Therapy

Cell culture pO ₂ monitoring with a fiber optic oxygen sensor

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On Tissue Oxygen and Hypoxia

Cited by 4 publications

References 12 publications

In vitro cell culture pO2 is significantly different from incubator pO2

In vitro cell culture pO2 is significantly different from incubator pO2

Spatial-Temporal Dose Metric Sampling for Explicit Photodynamic Therapy

Cell culture pO 2 monitoring with a fiber optic oxygen sensor

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Product

Resources

About

In vitro cell culture pO₂ is significantly different from incubator pO₂

In vitro cell culture pO₂ is significantly different from incubator pO₂

Cell culture pO ₂ monitoring with a fiber optic oxygen sensor