The present article introduce 0 (1,2,3) (Kolmogorov), 1 (1,2,3) (Fréchet), 2 (1,2,3) (Hausdorff), ℛ (1,2,3) (-regular), (1,2,3) (-normal), 0 (1,2,3) , 1 (1,2,3) and 2 (1,2,3) separation axioms in fuzzifying tri-topological spaces and studying the relation among them and also some of their properties.