Onchocerca flexuosa. sp. (Nematoda: Filarioidea) in Japanese Wild Sika Deer (Cervus nippon): Pathological and molecular identification

Abd-Ellatieff, Hoda A.; Bazh, Eman K.; Hussin, Shahinaz; Yamamoto, Ituto; Yanai, Tokuma; Abou-Rawash, Abdel-Rahman

doi:10.1007/s12639-021-01453-3

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…have been previously described in Neotropical primates causing mild peritonitis or chronic pleuritis, which are associated with areas of fibrous and occasionally, fibrinous adhesions (Chalifoux 1993 ; Strait et al 2012 ). Similarly, other pathological findings (e.g., polyserositis, peritonitis, eosinophilic or lymphocytic infiltration) have been associated with filarial nematodes (i.e., Setaria tundra , Onchocerca flexuosa ) in other hosts such as cervids from Finland, Japan, and Poland (Laaksonen et al 2009 ; Kowal et al 2013 ; Abd-Ellatieff et al 2022 ). However, further description of the lesions was not performed to confirm whether the injuries were caused by the presence of the nematodes in the abdominal cavity (Laaksonen et al 2007 ; Nikander et al 2007 ).…”

Section: Discussionmentioning

confidence: 98%

Pathological findings associated with Dipetalonema spp. (Spirurida, Onchocercidae) infection in two species of Neotropical monkeys from Brazil

et al. 2023

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Among vector-borne helminths, filarioids of the genus Dipetalonema (Spirurida: Onchocercidae) localize in several tissues and body cavities of several animal species, causing mild to moderate lesions. The pathological findings associated with Dipetalonema spp. infection in Neotropical monkeys from southern Brazil are herein described, along with a fatal case due to filarial polyserositis and entrapment of an intestinal segment. At necropsy, nematodes were observed in abdominal and thoracic cavities, or in the pericardium of 37 (31.3%) out of the 118 individuals examined (i.e., 35 Alouatta guariba clamitans and two Sapajus nigritus). In addition, at histology, 27.0% of positive animals presented microfilarie (inside blood vessels of lung, spleen, liver, and brain) and 8.1% presented adult nematodes in the heart, lung, and liver. In two cases, cross-sections of filarioids were associated with areas of epicardial thickening with intense fibrosis and pyogranulomatous inflammation in the brain, heart, liver, lungs, or spleen. The DNA fragment was amplify using the cox1 gene, sequenced and analyzed to identify the nematode species collected; presence of Wolbachia was assessed in the filarioids using the 16S rRNA gene. At BLAST analysis of the cox1 gene, 10 sequences showed 91.7% nucleotide identity with Dipetalonema gracile, and two with D. gracile (98.5%) and Dipetalonema graciliformis (98.3%). Phylogenetic analyses clustered sequences of the cox1 obtained in this study in two clades corresponding with the host species. Wolbachia sp. endosymbiont was detected in four samples. Data herein reported provide a description of pathological lesions associated with the infection by Dipetalonema spp., suggesting that they may cause disease in Neotropical monkeys. In addition, a better understanding of diversity and biology of Dipetalonema spp. in South America is needed to assess the impact they may cause in native non-human primates from Brazil.

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Section: Discussionmentioning

confidence: 98%

Pathological findings associated with Dipetalonema spp. (Spirurida, Onchocercidae) infection in two species of Neotropical monkeys from Brazil

et al. 2023

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“…Genetic variability between O. flexuosa from two continents (Europe, Asia) at the cox 1 locus exists ( Fig. 15 ) but only a single sequence is available from Asia (Japan) ( Abd-Ellatieff et al, 2022 ). 12S rDNA sequences only exist from European O. flexuosa isolates, without a geographic clustering.…”

Section: Discussionmentioning

confidence: 99%

“…Onchocerca flexuosa and O. jakutensis have been described from several European countries ( Bosch et al, 2016 ; Boijsen et al, 2017 ; Nielsen et al, 2022 ) and O. flexuosa very recently also from Japan ( Abd-Ellatieff et al, 2022 ). Onchocerca skrjabini has been described from a few European countries, including Germany ( Bain and Schulz-Key, 1974 ), Finland ( Bylund et al, 1981 ), the Czech Republic ( Baruš, 1994 ), Ukraine ( Zhelizniak, 2003 ) and from Japan ( Yagi et al, 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Morphological description and multilocus genotyping of Onchocerca spp. in red deer (Cervus elaphus) in Switzerland

Manzanell¹,

Stocker²,

Deplazes³

et al. 2022

International Journal for Parasitology: Parasites and Wildlife

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Zoonotic infection caused by Onchocerca japonica (Nematoda: Filarioidea) in a 69-year-old woman in Kanto Region, Eastern Honshu, Japan

Okazaki¹,

Fukuda²,

Hebisawa³

et al. 2022

Parasitology International

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Onchocerca flexuosa. sp. (Nematoda: Filarioidea) in Japanese Wild Sika Deer (Cervus nippon): Pathological and molecular identification

Cited by 3 publications

References 32 publications

Pathological findings associated with Dipetalonema spp. (Spirurida, Onchocercidae) infection in two species of Neotropical monkeys from Brazil

Pathological findings associated with Dipetalonema spp. (Spirurida, Onchocercidae) infection in two species of Neotropical monkeys from Brazil

Morphological description and multilocus genotyping of Onchocerca spp. in red deer (Cervus elaphus) in Switzerland

Zoonotic infection caused by Onchocerca japonica (Nematoda: Filarioidea) in a 69-year-old woman in Kanto Region, Eastern Honshu, Japan

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