Oncogene c-MYC Controls the Expression of PHF10 Subunit of PBAF Chromatin Remodeling Complex in SW620 Cell Line

Tatarskiy, E V; Georgiev, G.P.; Soshnikova, Natalia

doi:10.1134/s1607672919010204

Cited by 3 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The above data indicated that circ_0001023 might serve as a ceRNA to modulate miR-409-3p/PHF10 axis during GC progression. Considering that PHF10 is a crucial component of the PBAF (SWI/SNF) multiprotein complex, which changes the chromatin structure and dominates multiple eukaryotic gene expression, 24 it is possible that circ_0001023 has the potential to indirectly regulate more downstream oncogenes to drive GC progression, which needs to be investigated in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Plant homeodomain finger 10 (PHF10), belonging to the zinc finger protein family, can repress the transcription of caspase-3 and impaired the apoptosis pathway in GC cells, so as to promote the growth of tumor. 21 PHF10 was significantly overexpressed in GC and colonic cancer tissues, [22][23][24] indicating that PHF10 plays an oncogenic role in the progression of cancers. In addition, it is reported that PHF10 is negatively regulated miR-409-3p in GC.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

<p>Circ_0001023 Promotes Proliferation and Metastasis of Gastric Cancer Cells Through miR-409-3p/PHF10 Axis</p>

Wang

Chen

Gao

2020

OTT

View full text Add to dashboard Cite

Background: Circular RNAs (circRNAs) have been well documented to regulate the gene expression via sponging microRNA (miRNA) in diverse neoplasms including gastric cancer (GC). Methods: In the present study, the expressions of circ_0001023, miR-409-3p, and plant homeodomain finger 10 (PHF10) in GC tissues were detected by qRT-PCR. Chi-square test was performed to analyze the associations between circ_0001023 and pathological parameters. Cell Counting Kit-8 assay, colony formation assay, flow cytometry, and transwell assay were adopted to detect the role of circ_0001023/miR-409-3p axis in the proliferation, apoptosis, and migration of GC cells, respectively. The targeting relationship between circ_0001023 and miR-409-3p was investigated by dual-luciferase gene reporter gene assay. Additionally, subcutaneous xenotransplanted tumor model in nude mice was established to detect the function of circ_0001023 on GC growth in vivo. Results: Compared with adjacent tissues, the expression of circ_0001023 was significantly upregulated and correlated with lymph node invasion and higher T stage of GC patients. It has also been proved that circ_0001023 could target miR-409-3p. Silencing circ_0001023 can impede the proliferation of GC cells and promote apoptosis, while miR-409-3p inhibitors can partially reverse the biological behavior of GC cells mentioned above. Moreover, the expression of circ_0001023 was reversely associated with miR-409-3p expression but positively correlated with PHF10, a downstream oncogene of miR-409-3p. Conclusion: Collectively, it is concluded that circ_0001023 promotes the progression of GC via regulating miR-409-3p/PHF10 axis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

<p>Circ_0001023 Promotes Proliferation and Metastasis of Gastric Cancer Cells Through miR-409-3p/PHF10 Axis</p>

Wang

Chen

Gao

2020

OTT

View full text Add to dashboard Cite

show abstract

“…Сниженная экспрессия PHF10 при раке почки коррелирует с повышенной выживаемостью пациентов [64,66], что может быть связано с положительным влиянием онкогена с-МYС на экспрессию PHF10 [68].…”

Section: Dpf взаимодействуют с ацилированными H3k14 и H3k9unclassified

The DPF Domain As a Unique Structural Unit Participating in Transcriptional Activation, Cell Differentiation, and Malignant Transformation

et al. 2020

Self Cite

View full text Add to dashboard Cite

The DPF (double PHD finger) domain consists of two PHD fingers organized in tandem. The two PHD-finger domains within a DPF form a single structure that interacts with the modification of the N-terminal histone fragment in a way different from that for single PHD fingers. Several histone modifications interacting with the DPF domain have already been identified. They include acetylation of H3K14 and H3K9, as well as crotonylation of H3K14. These modifications are found predominantly in transcriptionally active chromatin. Proteins containing DPF belong to two classes of protein complexes, which are the transcriptional coactivators involved in the regulation of the chromatin structure. These are the histone acetyltransferase complex belonging to the MYST family and the SWI/SNF chromatin-remodeling complex. The DPF domain is responsible for the specificity of the interactions between these complexes and chromatin. Proteins containing DPF play a crucial role in the activation of the transcription of a number of genes expressed during the development of an organism. These genes are important in the differentiation and malignant transformation of mammalian cells.

show abstract

Construction of the gene regulatory network identifies MYC as a transcriptional regulator of SWI/SNF complex

Srikanth

Ramachandran

Mohan

2020

Sci Rep

View full text Add to dashboard Cite

Precise positioning of nucleosomes at the gene regulatory elements mediated by the SWI/SNF family of remodelling complex is important for the transcriptional regulation of genes. A wide set of genes are either positively or negatively regulated by SWI/SNF. In higher eukaryotes, around thirty genes were found to code for SWI/SNF subunits. The construction of a gene regulatory network of SWI/SNF subunits identifies MYC as a common regulator for many of the SWI/SNF subunit genes. A meta-analysis study was conducted to investigate the MYC dependent regulation of SWI/SNF remodelling complex. Subunit information and the promoter sequences of the subunit genes were used to find the canonical E-box motif and its variants. Detailed analysis of mouse and human ChIP-Seq at the SWI/SNF subunit loci indicates the presence of MYC binding peaks overlapping with E-boxes. The co-expression correlation and the differential expression analysis of wt vs. MYC perturbed MEFs indicate the MYC dependent regulation of some of the SWI/SNF subunits. The extension of the analysis was done on MYC proficient and MYC deficient embryonic fibroblast cell lines, TGR1 and HO15, and in one of the MYC amplified cancer types, Medulloblastoma. A transcriptional regulatory feedback loop between MYC and SWI/SNF could be a major factor contributing to the aggressiveness of MYC dependent cancers.

show abstract

Oncogene c-MYC Controls the Expression of PHF10 Subunit of PBAF Chromatin Remodeling Complex in SW620 Cell Line

Cited by 3 publications

References 7 publications

<p>Circ_0001023 Promotes Proliferation and Metastasis of Gastric Cancer Cells Through miR-409-3p/PHF10 Axis</p>

<p>Circ_0001023 Promotes Proliferation and Metastasis of Gastric Cancer Cells Through miR-409-3p/PHF10 Axis</p>

The DPF Domain As a Unique Structural Unit Participating in Transcriptional Activation, Cell Differentiation, and Malignant Transformation

Construction of the gene regulatory network identifies MYC as a transcriptional regulator of SWI/SNF complex

Contact Info

Product

Resources

About