Oncogenic Potential of Mouse Translation Elongation Factor-1δ, a Novel Cadmium-responsive Proto-oncogene

Joseph, Pius; Lei, Yixiong; Whong, Wen‐Zong; Ong, Tong-man

doi:10.1074/jbc.m109373200

Cited by 59 publications

(38 citation statements)

References 31 publications

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“…More recent reports have indicated that EF-1 delta can function as a proto-oncogene when overexpressed (Joseph et al, 2002;Lei et al, 2002). Joseph et al (2002) indicated that overexpression of EF-1 delta protein by transfection was oncogenic in NIH3T3 cells, as evidenced by the appearance of transformed foci exhibiting anchorage-independent growth and the potential to grow as tumours in nude mice.…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma

et al. 2004

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Elongation factor-1 (EF-1) delta is a subunit of EF-1, which is a protein complex that participates in the elongation step of mRNA translation and has recently been considered to correlate with oncogenic transformation. However, there has been no information regarding the clinical significance of EF-1 delta mRNA expression in malignant tumours, including oesophageal carcinoma. Thus, we quantitated the expression of EF-1 delta in malignant and benign oesophageal tissues and associated these levels with clinicopathologic parameters of oesophageal carcinoma. Paired oesophageal tissue samples from cancerous and corresponding noncancerous parts were obtained from 52 patients who underwent curative oesophagectomy. Quantitative analyses of EF-1 delta expression were performed using real-time quantitative reverse transcription -polymerase chain reaction. Elongation factor-1 delta mRNA overexpression in cancerous tissues compared to normal counterparts was observed in 38 of 52 (73%) patients. The mean expression level of EF-1 delta mRNA in cancerous tissues was significantly higher than that in noncancerous tissues (Po0.01). A higher expression of EF-1 delta was significantly correlated with lymph node metastases (Po0.05) and advanced stages (Po0.05). Furthermore, the cause-specific survival of patients with a higher expression of EF-1 delta was significantly poorer than those with a lower expression (5-year cause-specific survival rates; 23 and 54%, respectively, Po0.05). The results of this study indicated that EF-1 delta mRNA expression was significantly higher in cancerous compared to noncancerous oesophageal tissues, and a higher expression of EF-1 delta mRNA was correlated with lymph node metastases, advanced disease stages and poorer prognosis for patients with oesophageal carcinoma.

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Section: Discussionmentioning

confidence: 99%

Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma

et al. 2004

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“…Altering the expression level or the function of eIF3 may influence the synthesis of some proteins and consequently cause abnormal cell growth and malignant transformation. eIF3 comprises at least 12 non-identical subunits of which six have been implicated in human cancers (eIF3a, b, c, h, i and e) (Joseph et al, 2002;Watkins and Norbury, 2002). eIF3f is another eIF3 subunit whose function is not known.…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells

et al. 2006

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The eukaryotic initiation factor 3f (eIF3f) is the p47 subunit of the multi-subunit eIF3 complex. eIF3 plays an important role in translation initiation. In the present study, we investigate the biological function of eIF3f in translation and apoptosis in tumor cells. We demonstrated for the first time that eIF3f is downregulated in most human tumors using a cancer profiling array and confirmed by real-time reverse transcription PCR in melanoma and pancreatic cancer. Overexpression of eIF3f inhibits cell proliferation and induces apoptosis in melanoma and pancreatic cancer cells. Silencing of eIF3f protects melanoma cells from apoptosis. We further investigated the biological function of eIF3f. In vitro translation studies indicate that eIF3f is a negative regulator of translation and that the region between amino acids 170 and 248 of eIF3f is required for its translation regulatory function. Ectopic expression of eIF3f inhibits translation and overall cellular protein synthesis. Ribosome profile and ribosomal RNA (rRNA) fragmentation assays revealed that eIF3f reduces ribosomes, which may be associated with rRNA degradation. We propose that eIF3f may play a role in ribosome degradation during apoptosis. These data provide critical insights into the cellular function of eIF3f and in linking translation initiation and apoptosis.

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“…Eukaryotic translation elongation factor 1d Eukaryotic translation elongation factor 1d (TEF1d) is a newly discovered protein (Joseph et al, 2002) that has been shown to be a protooncogene causing tumourigenetic growth in nickel-treated human bronchial epithelial cells (Lei et al, 2006). High expression of TEF1d was detected in NSCLC (Liu et al, 2004), which is consistent with our data.…”

Section: Gdp Dissociation Inhibitors Gdp Dissociation Inhibitors (Rhomentioning

confidence: 99%

Differential tissue-specific protein markers of vaginal carcinoma

et al. 2009

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The objective was to identify proteins differentially expressed in vaginal cancer to elucidate relevant cancer-related proteins. A total of 16 fresh-frozen tissue biopsies, consisting of 5 biopsies from normal vaginal epithelium, 6 from primary vaginal carcinomas and 5 from primary cervical carcinomas, were analysed using two-dimensional gel electrophoresis (2-DE) and MALDI-TOF mass spectrometry. Of the 43 proteins identified with significant alterations in protein expression between non-tumourous and tumourous tissue, 26 were upregulated and 17 were downregulated. Some were similarly altered in vaginal and cervical carcinoma, including cytoskeletal proteins, tumour suppressor proteins, oncoproteins implicated in apoptosis and proteins in the ubiquitin -proteasome pathway. Three proteins were uniquely altered in vaginal carcinoma (DDX48, erbB3-binding protein and biliverdin reductase) and five in cervical carcinoma (peroxiredoxin 2, annexin A2, sarcomeric tropomyosin kappa, human ribonuclease inhibitor and prolyl-4-hydrolase beta). The identified proteins imply involvement of multiple different cellular pathways in the carcinogenesis of vaginal carcinoma. Similar protein alterations were found between vaginal and cervical carcinoma suggesting common tumourigenesis. However, the expression level of some of these proteins markedly differs among the three tissue specimens indicating that they might be useful molecular markers.

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Oncogenic Potential of Mouse Translation Elongation Factor-1δ, a Novel Cadmium-responsive Proto-oncogene

Cited by 59 publications

References 31 publications

Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma

Clinical significance of elongation factor-1 delta mRNA expression in oesophageal carcinoma

Decreased expression of eukaryotic initiation factor 3f deregulates translation and apoptosis in tumor cells

Differential tissue-specific protein markers of vaginal carcinoma

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