Oncogenic transformation by the tax gene of human T-cell leukemia virus type I in vitro.

Abstract

Human T-cell leukemia virus type I (HTLV-

HTLV-1 Tax protein sensitizes cells to apoptotic cell death induced by DNA damaging agents

“…CREF cells were chosen because we have previously demonstrated suppression of DNA repair following transient transfection of Tax into CREF cells, and rat ®broblast cells have been used extensively to study Tax-mediated transformation (Matsumoto et al, 1997;Smith and Greene, 1991;Tanaka et al, 1990;Yamaoka et al, 1996). Western blot analysis was performed to examine Tax expression in these cells prior to the repair assays.…”

Functional interaction of the HTLV-1 transactivator Tax with activating transcription factor-4 (ATF4)

“…The Tax protein of HTLV-1 is a potent transactivator of viral gene expression (Cann et al, 1985;Felber et al, 1985;Fujisawa et al, 1985) and is capable of immortalizing human T lymphocytes in vitro (Grassmann et al, 1989;Tanaka et al, 1990). Tax is a 40 kDa nuclear protein that activates viral transcription through an enhancer element (Tax responsive element: TRE) consisting of three 21 bp repeats located within the HTLV-1 LTR (Sodroski et al, 1984;Felber et al, 1985;Brady et al, 1987;Paskalis et al, 1986).…”

Inhibition of proliferation and CD25 down-regulation by retinoic acid in human adult T cell leukemia cells

“…HTLV-I genome contains gag, pol and env genes, as well as a region referred to as pX, 10 which encodes the proteins Tax, Rex, and P21 x respectively. 11 Tax is a nuclear protein that Reagents transactivates its cognate long terminal repeat (LTR), 12 immortalizes rat embryo fibroblasts and primary T lymphocytes, 13 PMA, 13-cis RA, and ATRA were obtained from Sigma (St and induces apoptosis of W4 cells generated by the transfecLouis, MO, USA), and PHA was from DIFCO (Detroit, MI, tion of Rat-1 cells with a Tax expression plasmid. 14 The IL-USA).…”