Oncogenic Transformation<i>in Vitro</i>after Split-dose X-irradiation

Terzaghi, Margaret; Little, John B.

doi:10.1080/09553007614550691

Cited by 23 publications

(5 citation statements)

References 3 publications

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“…Similar results have been reported by Terzaghi and Little (1976), with iii vitro malignant transformation experiments in which the transformation frequency in 1OT-1/2 mouse embryo fibroblasts following single-or split-dose irradiation with various doses of X-rays was examined.…”

Section: Resultssupporting

confidence: 70%

“…To compare these results with those obtained in in vitro malignant transformation experiments (Borek and Hall 1974;Terzaghi and Little 1976), the mutation frequencies in prototrophic CH-hai Cl 23 cells following single-or split-dose irradiation with X-rays less than 1,000 R in total doses are shown in Table 1. In split-dose groups, an interval of 24 hours between the two doses was adopted, as these were an apparent complete recovery by this time.…”

Section: Resultsmentioning

confidence: 99%

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Repair of radiation-induced lethal and mutational damage in chinese hamster cells in vitro.

Suzuki

Hoshi

Horikawa

1979

The Japanese journal of genetics

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The ability of Chinese hamster hai cells to repair damage related to cell death and mutational change induced by X-rays was studied by using a sensitive forward mutation system from prototrophic CH-hai Cl 23 cells to auxotrophs.The results obtained from the dose rate and split-dose experiments suggest that Chinese hamster hai cells have repair mechanisms for the sublethal and mutational damage induced by X-rays and that these mechanisms act in common for repair of both types of damage.In addition, we also found that these cells have repair abilities for lethal and mutational damage induced by a potentially lethal dose of radiation.

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Section: Resultssupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Repair of radiation-induced lethal and mutational damage in chinese hamster cells in vitro.

Suzuki

Hoshi

Horikawa

1979

The Japanese journal of genetics

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“…While there are many transformation systems available the C3H10T-system was selected because it is not based on subtle changes in colony morphology and has been used to detect many chemical and physical carcinogens (Jones et al, 1976;Benedict et al, 1979;Chan & Little, 1976;Terzaghi & Little, 1976). One important consideration is that this test takes place on the base of the culture vessel where cells and particulates may interact; in those systems using soft agar suspensions the cells and dusts may not have intimate contact, or if contact were made then the particulate material in the agar could provide anchorage points for the growth of normal (nontransformed) cells.…”

Section: Discussionmentioning

confidence: 99%

In vitro genotoxic activities of fibrous erionite

Poole¹,

Brown²,

Turver³

et al. 1983

Br J Cancer

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A high incidence of mesothelioma has been reported from some villages in Cappadocia, Turkey. This type of cancer is usually associated with the inhalation of asbestos, but on the basis of the most prevalent fibre in the dust from these villages, the Turkish outbreak has been attributed to the inhalation of zeolite fibres. A counter hypothesis, based on the detection of very small quantities of chrysotile and tremolite in strata samples and human lung tissue, postulates a significant role of these minerals as one of several factors contributing to pleural disease. A respirable fraction of erionite, (from Oregon, USA, but with similar characteristics to the fibres found in Turkey), has some in vitro genotoxic properties associated with many conventional carcinogens. In this study these fibres caused an increase in morphological transformation and unscheduled DNA repair synthesis (UDS) in C3H10T1/2 cells and UDS in the human lung cell line--A549. It is therefore suggested that exposure to fibrous erionite alone may be sufficient to cause the high incidence of pleural tumours observed in Turkey. Images Figure 1 Figure 3

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“…The high-dose plateaus predicted by the repair dependent model have been observed in both UV and ionizing radiation transformation frequency data from multiple laboratories (Chan and Little 1976, Terzaghi and Little 1976a, 1976b, Han and Elkind 1979, Suzuki et al 1981, thus supporting the relevance of the model. A high dose plateau is an inherent consequence (Han and Elkind 1979).…”

Section: Discussionmentioning

confidence: 73%

Repair-dependent cell radiation survival and transformation: an integrated theory

Sutherland

2014

Phys. Med. Biol.

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The repair-dependent model of cell radiation survival is extended to include radiation-induced transformations. The probability of transformation is presumed to scale with the number of potentially lethal damages that are repaired in a surviving cell or the interactions of such damages. The theory predicts that at doses corresponding to high survival, the transformation frequency is the sum of simple polynomial functions of dose; linear, quadratic, etc, essentially as described in widely used linear-quadratic expressions. At high doses, corresponding to low survival, the ratio of transformed to surviving cells asymptotically approaches an upper limit. The low dose fundamental- and high dose plateau domains are separated by a downwardly concave transition region. Published transformation data for mammalian cells show the high-dose plateaus predicted by the repair-dependent model for both ultraviolet and ionizing radiation. For the neoplastic transformation experiments that were analyzed, the data can be fit with only the repair-dependent quadratic function. At low doses, the transformation frequency is strictly quadratic, but becomes sigmodial over a wider range of doses. Inclusion of data from the transition region in a traditional linear-quadratic analysis of neoplastic transformation frequency data can exaggerate the magnitude of, or create the appearance of, a linear component. Quantitative analysis of survival and transformation data shows good agreement for ultraviolet radiation; the shapes of the transformation components can be predicted from survival data. For ionizing radiations, both neutrons and x-rays, survival data overestimate the transforming ability for low to moderate doses. The presumed cause of this difference is that, unlike UV photons, a single x-ray or neutron may generate more than one lethal damage in a cell, so the distribution of such damages in the population is not accurately described by Poisson statistics. However, the complete sigmodial dose-response data for neoplastic transformations can be fit using the repair-dependent functions with all parameters determined only from transformation frequency data.

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Oncogenic Transformationin Vitroafter Split-dose X-irradiation

Cited by 23 publications

References 3 publications

Repair of radiation-induced lethal and mutational damage in chinese hamster cells in vitro.

Repair of radiation-induced lethal and mutational damage in chinese hamster cells in vitro.

In vitro genotoxic activities of fibrous erionite

Repair-dependent cell radiation survival and transformation: an integrated theory

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