Oncogenic Wnt3a: a promising specific biomarker in hepatocellular carcinoma

Yao, Min; Miao, Fei; Zheng, Wenjie; Yao, Deng-Fu

doi:10.20517/2394-5079.2018.32

Cited by 6 publications

(2 citation statements)

References 77 publications

(103 reference statements)

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“…The Human Wnt3a gene located on the chromosome and the canonical Wnt (1q42.13) has been regarded as activating the β-catenin accumulation signaling pathway (24,59). The Wnt3a protein is a member of the Wnt family that triggers the activation of the canonical Wnt signaling pathway (22).…”

Section: Discussionmentioning

confidence: 99%

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

Hemn Abdalla Omer,

Kawa Amin

2023

Cell Mol Biol (Noisy-le-grand)

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The objective of the current investigation was to investigate the diagnostic utility of the serum concentrations and mutational status of TGFβ1, SMAD2, CTNNβ1, and Wnt3a. and the expression levels of human-related genes in patients with non-small cell lung cancer (NSCLC). The serum concentrations were determined using the ELISA technique, and PCR for genotype variations of TGFβ1, SMAD2, CTNNβ1, and Wnt3a were examined using Sanger sequencing in tissue samples obtained from 93 patients with NSCLC and 84 healthy individuals for blood, and 20 Formalin Fixed Paraffin Embedded (FFPE) from normal samples dissected adjacent to the tumour. The findings of the current investigation indicate that individuals diagnosed with NSCLC exhibited significant elevation in the serum levels of CEA and CYFRA21-1, as well as TGFβ1, SMAD2, CTNNβ1, and Wnt3a. In total, 325 mutations in four trialled genes (243 mutations in TGFβ1, 24 mutations in SMAD2,47 mutation Wnt3a and 11 mutations in CTNNβ1) were identified in patients with NSCLC. Furthermore, all mutations were recorded in adenocarcinoma, not squamous and normal adjacent tumour cells. CYFRA21-1 and CEA are more significant between NSCLC and HC, gender, and NSCLC types (p<0.001). In detail, TGFβ1 exhibited the highest rate of mutations among other genes and three types of genomic mutations. Elevated levels and genetic polymorphisms of TGFβ1, SMAD2, CTNNβ1, and Wnt3a may play crucial functions in the pathogenesis and angiogenesis of non-small cell lung cancer (NSCLC). These biomarkers might play a role in future immunologic response and pharmacologically targeted NSCLC therapy.

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Section: Discussionmentioning

confidence: 99%

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

Hemn Abdalla Omer,

Kawa Amin

2023

Cell Mol Biol (Noisy-le-grand)

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“…The Wnt signaling pathway is involved in important life activities such as organ formation and early development through signal transduction regulated by Wnt genes. Many Wnt genes have been observed in many higher eukaryotes, ranging from humans to Xenopus and zebrafish [ 6 , 7 , 8 , 9 ]. Further, Wnt signaling genes related to turtles’ gonadal development and differentiation have been identified, such as Wnt4 and Rspo1 [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Analysis of Wnt2 Gene in the Sex Differentiation of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Zhou

Zhang

Chen

et al. 2023

Life

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The Chinese soft-shelled turtle (Pelodiscus sinensis) is an important freshwater aquaculture animal in China. The Wnt gene family plays important regulatory roles in the development and growth of mammals. However, the precise function of these family genes has not been well understood in the sex differentiation of Chinese soft-shelled turtles. Here, we cloned a member of the Wnt family, Wnt2, which obtained a 1077 bp open reading frame that encoded a 358-aa protein. The putative amino acid sequences of proteins are exceeded 80% identical to other turtles. The expression level of Wnt2 peaked at the 14th stage both in female and male embryos during the early gonadal differentiation period of Chinese soft-shelled turtles, which occurred before gonadal differentiation. Wnt2 mRNA was expressed at higher levels in the brains and gonads of mature P. sinensis females compared with those in mature males. Wnt agonists significantly affected the expression level of Wnt2 during the gonadal differentiation period. After Wnt agonists (1.0 μg/μL, 2.5 μg/μL, 5.0 μg/μL) treatment, the expression level of the Wnt2 generally appeared to have an inverted-V trend over time in female embryonic gonads. The results suggested that Wnt2 may participate in the regulation of gonad development in P. sinensis during the early embryonic stages. These results could provide a theoretical basis for the reproduction process of the Chinese soft-shelled turtle.

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Wnt3a is a promising target in colorectal cancer

Tufail¹,

Wu²

2023

Med Oncol

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Oncogenic Wnt3a: a promising specific biomarker in hepatocellular carcinoma

Cited by 6 publications

References 77 publications

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

TGFβ1, SMAD2, CTNNβ1, and Wnt3a gene mutational status and serum concentrations in individuals with non-small cell lung cancer

Identification and Expression Analysis of Wnt2 Gene in the Sex Differentiation of the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Wnt3a is a promising target in colorectal cancer

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