Oncoprotein HCCR-1 expression in breast cancer is well correlated with known breast cancer prognostic factors including the HER2 overexpression, p53 mutation, and ER/PR status

Abstract

BackgroundOncoprotein HCCR-1 functions as a negative regulator of the p53 and contributes breast tumorigenesis. The serum HCCR-1 assay is useful in diagnosing breast cancer and mice transgenic for HCCR developed breast cancers. But it is unknown how HCCR-1 contributes to human breast tumorigenesis.MethodsOncogene HCCR-1 expression levels were determined in normal breast tissues, breast cancer tissues and cancer cell lines. We examined whether HCCR-1 protein expression in breast cancer is related to different biological characteristics, including ER, PR, p53 genotype, and HER2 status in 104 primary breast cancer tissues using immunohistochemical analyses.ResultsHCCR-1 was upregulated in breast cancer cells and tissues compared with normal breast tissues. In this study, overexpression of HCCR-1 was well correlated with known breast cancer prognostic markers including the presence of steroid receptors (ER and PR), p53 mutation and high HER2 overexpression. HCCR-1 was not detected in the ER-negative, PR-negative, p53 negative and low HER2 breast cancer tissues. These data indicate that the level of HCCR-1 in breast cancer tissues is relatively well correlated with known breast cancer factors, including the HER2 overexpression, p53 mutation, and ER/PR status.ConclusionDetermination of HCCR-1 levels as options for HER2 testing is promising although it needs further evaluation.

Quantitative detection of the human cervical cancer oncogene for monitoring the minimal residual disease in acute leukemia

“…16,17 Accumulated data demonstrate that HCCR was not only over-expressed in human cervical cancer tissues but also found to have high-level expression in various human malignancies including breast, kidney, stomach, colon, liver, and ovarian cancer. [15][16][17][18][19][20][21] The functional role of HCCR in tumorigenesis may be related to the negative regulation of the p53 tumor suppressor gene. 16,21 HCCR1-or HCCR2-transgenic nude mice could form spontaneous breast cancers, which further confirmed the critical role of HCCR in tumorigenesis.…”

“…16 Although several published data have shown that HCCR expression in some solid tumors is correlated to clinical outcome and confirmed it as a good biomarker of monitoring disease progression, its specific role in leukemia remains elusive. [18][19][20] In the present study, we investigated the mRNA levels of HCCR in patients with AL using the real-time RT-PCR method and evaluated whether quantitative detection of HCCR gene is useful for monitoring MRD in AL.…”

ER81-shRNA Inhibits Growth of Triple-negative Human Breast Cancer Cell Line MDA-MB-231 In Vivo and in Vitro

“…The MDA-MB-231 cell line (American Type Culture Collection number: HTB-26) was established from human metastatic mammary carcinoma cells showing high expression levels of ER81 (Baert et al,1997)and no expression of HER2, ER and PR (Ha et al, 2009;Subik et al, 2010) and it can differentiate into poorly differentiated adenocarcinoma (grade III) in nude mice and antilymphocyte serum-treated BALB/c mice. The cell line was purchased from the cell line bank at Kunming Institute of Zoology, Chinese Academy of Sciences.…”

Analysis of GPER1, CXCR1 and HER-2 Immuno Expression in Follicular Thyroid Lesions and their Possible Role as Diagnostic Markers

“…HER-2 contributes to physiological mechanisms of cell proliferation and differentiation by intrinsic tyrosine-kinase activity. In malignant tumours, HER-2 over expression is associated with increased disease recurrence and poor prognosis [20,21]. The importance of HER-2 in cancer has emerged by the effectiveness of the anti-HER-2 humanized monoclonal antibody trastuzumab (Herceptin), especially when combined with chemotherapy, for the treatment of HER2-overexpressing breast cancers.…”