Oncoprotein
            <scp>CIP</scp>
            2A promotes the disassembly of primary cilia and inhibits glycolytic metabolism

Jeong, Ae Lee; Ka, Hye In; Han, Sora; Lee, Sunyi; Lee, Eun‐Woo; Soh, Sujung; Joo, Hyun Jeong; Sumiyasuren, Buyanravjkh; Park, Ji Young; Lim, Jong-Seok; Park, Jong Hoon; Lee, Myung Sok; Yang, Young

doi:10.15252/embr.201745144

Cited by 13 publications

(11 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RPE1 cells were cultivated in DMEM-F12 1:1 (Invitrogen) supplemented with 10% of fetal bovine serum (FBS, PAN TM BIO-TECH), and 1% penicillin and streptomycin. To induce quiescence, RPE1 cells were washed twice with DPBS and incubated for 48 h with serum-free DMEM-F12 97 . To repress protein synthesis in quiescent RPE1 cell, 100 μg/ml of cycloheximide (TOKU-E) and 300 μM of puromycin (InVivoGen) were added for 2 h, and 100 μg/ml of puromycin and 200 μg/ml of cycloheximide were treated for 6 h to proliferating RPE1 cell before fixation.…”

Section: Methodsmentioning

confidence: 99%

Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

et al. 2021

View full text Add to dashboard Cite

Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division and is linked to human neurodevelopmental disorders. In quiescent mNSC and immortalized human retinal pigment epithelial (RPE1) cells, centrioles form a basal body for ciliogenesis. Here, we report that EJCs accumulate at basal bodies of mNSC or RPE1 cells and decline when these cells differentiate or resume growth. A high-throughput smFISH screen identifies two transcripts accumulating at centrosomes in quiescent cells, NIN and BICD2. In contrast to BICD2, the localization of NIN transcripts is EJC-dependent. NIN mRNA encodes a core component of centrosomes required for microtubule nucleation and anchoring. We find that EJC down-regulation impairs both pericentriolar material organization and ciliogenesis. An EJC-dependent mRNA trafficking towards centrosome and basal bodies might contribute to proper mNSC division and brain development.

show abstract

Section: Methodsmentioning

confidence: 99%

Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…CEP192 is required for MT nucleation by interphase centrosomes (76,310), and AurA and PLK1 are involved in the suppression of ciliogenesis (268,(311)(312)(313)(314)(315). In this regard, the recently identified functional partner of CEP192, cancerous inhibitor of PP2A (CIP2A), promotes primary cilia disassembly through AurA activation (316,317). Moreover, PCM maintenance and centriole stability in interphase cells have been suggested to require PLK1 activity (318).…”

Section: Key Role Of Aura-plk Signaling In the Centrosome Cyclementioning

confidence: 99%

Aurora-PLK1 cascades as key signaling modules in the regulation of mitosis

Joukov¹,

2018

View full text Add to dashboard Cite

Mitosis is controlled by reversible protein phosphorylation involving specific kinases and phosphatases. A handful of major mitotic protein kinases, such as the cyclin B-CDK1 complex, the Aurora kinases, and Polo-like kinase 1 (PLK1), cooperatively regulate distinct mitotic processes. Research has identified proteins and mechanisms that integrate these kinases into signaling cascades that guide essential mitotic events. These findings have important implications for our understanding of the mechanisms of mitotic regulation and may advance the development of novel antimitotic drugs. We review collected evidence that in vertebrates, the Aurora kinases serve as catalytic subunits of distinct complexes formed with the four scaffold proteins Bora, CEP192, INCENP, and TPX2, which we deem "core" Aurora cofactors. These complexes and the Aurora-PLK1 cascades organized by Bora, CEP192, and INCENP control crucial aspects of mitosis and all pathways of spindle assembly. We compare the mechanisms of Aurora activation in relation to the different spindle assembly pathways and draw a functional analogy between the CEP192 complex and the chromosomal passenger complex that may reflect the coevolution of centrosomes, kinetochores, and the actomyosin cleavage apparatus. We also analyze the roles and mechanisms of Aurora-PLK1 signaling in the cell and centrosome cycles and in the DNA damage response.

show abstract

“…Besides this above-cited role of the primary cilium in the maintenance of mitochondrial functions, a metabolic shift towards glycolysis has been hypothesized, in correlation with the presence of primary cilia [172]. However, although a cancerous inhibitor of protein phosphatase 2A (CIP2A)-depleted cells showed an increase in primary cilia expression, and in glycolytic activity and capacity, and although the primary cilia seemed to be necessary for enhancing glycolytic activity, the increased glycolytic metabolism was independent to the presence of primary cilia.…”

Section: Primary Cilium Hypoxia and Cancer Hallmarksmentioning

confidence: 99%

Primary Cilium in Cancer Hallmarks

Fabbri

Bost

Mazure

2019

IJMS

View full text Add to dashboard Cite

The primary cilium is a solitary, nonmotile and transitory appendage that is present in virtually all mammalian cells. Our knowledge of its ultrastructure and function is the result of more than fifty years of research that has dramatically changed our perspectives on the primary cilium. The mutual regulation between ciliogenesis and the cell cycle is now well-recognized, as well as the function of the primary cilium as a cellular “antenna” for perceiving external stimuli, such as light, odorants, and fluids. By displaying receptors and signaling molecules, the primary cilium is also a key coordinator of signaling pathways that converts extracellular cues into cellular responses. Given its critical tasks, any defects in primary cilium formation or function lead to a wide spectrum of diseases collectively called “ciliopathies”. An emerging role of primary cilium is in the regulation of cancer development. In this review, we seek to describe the current knowledge about the influence of the primary cilium in cancer progression, with a focus on some of the events that cancers need to face to sustain survival and growth in hypoxic microenvironment: the cancer hallmarks.

show abstract

Oncoprotein CIP 2A promotes the disassembly of primary cilia and inhibits glycolytic metabolism

Cited by 13 publications

References 72 publications

Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

Exon junction complex dependent mRNA localization is linked to centrosome organization during ciliogenesis

Aurora-PLK1 cascades as key signaling modules in the regulation of mitosis

Primary Cilium in Cancer Hallmarks

Contact Info

Product

Resources

About