Oncornavirus: Isolation From a Squirrel Monkey (
            <i>Saimiri sciureus</i>
            ) Lung Culture

Heberling, R. L.; Barker, S. T.; Kalter, S. S.; Gc, Smith; Helmke, R

doi:10.1126/science.63993

Cited by 107 publications

(52 citation statements)

References 30 publications

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“…Approximately 20 ~o of the MPMV genome is present in the DNA of all tissues of normal Rhesus monkeys (Drohan et al I977), whereas the entire MPMV genome can be found in tissues of MPMVinfected monkeys (Colcher et al 1975)-These latter findings were the first to suggest that MPMV is a horizontally transmitted virus in macaque species. Other MPMV antigenically related retroviruses (Colcher et al I977;Hino et al I977;Devare et al I978;Fine et al 1978b) have been isolated from both a langur monkey (an Old World monkey; Todaro et al I978 ) and squirrel monkeys (a New World monkey; Heberling et al 1977). These retroviruses, which have been identified in several primate populations, appear in many species as complete endogenous proviral DNA sequences (Benveniste & Todaro, I977;Drohan et al I977;Fine et al I978b;Schochetman & Fine, 1978 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Infection and Replication of Mason-Pfizer Monkey Virus in Human Cell Cultures

Fine

Clarke

Arthur

1979

Journal of General Virology

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SUMMARYHuman cells derived from both normal and neoplastic tissues can be infected by Mason-Pfizer monkey virus (MPMV) without accompanying cytopathology. Infection of cell cultures such as human rhabdomyosarcoma (A2o4) results in a persistently infected cell line which can be subcultured over 3o sequential culture passages without significant change in phenotypic properties according to reverse, transcriptase (RT), MPMV p27 antigen content, virus particle count and infectivity titrel Productive virus infections were established at relatively low virus particle (VP) input multiplicities (p.i.m. ; about o.o6 VP/cell) in A2o4 cell cultures. At higher p.i.m. (about 6oo to 6ooo VP/cell) newly synthesized virus was detected within 4 days post infection. Although virus production was cumulative following primary infection, after subculture of infected cultures MPMV production was greater during active cell division. Using synchronization techniques, MPMV replication in persistently infected cultures was found to be cell cycle-dependent. The major internal antigen, P27, was synthesized in G2 and newly synthesized virus particles were released predominantly during mitosis and early G1. Colcemid arrest of cells during mitosis inhibited subsequent MPMV release. Consequently, production of extracellular virus depends upon the progression of cells through the mitotic stage. These data, which provided a basic understanding of the virushost relationship that occurs in primate cells productively infected with MPMV, were used as a guideline for isolating MPMV-like viruses from experimentally and naturally infected Rhesus monkeys.

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Section: Introductionmentioning

confidence: 99%

Characterization of Infection and Replication of Mason-Pfizer Monkey Virus in Human Cell Cultures

Fine

Clarke

Arthur

1979

Journal of General Virology

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“…Heberling et al (9) reported that a virus morphologically similar to MPMV could be isolated from squirrel monkey tissues by the cocultivation of those tissues with cells of another species. We have recently shown that the squirrel monkey retrovirus (SMRV) is an endogenous virus of squirrel monkeys (10).…”

mentioning

confidence: 99%

Interspecies radioimmunoassay for the major structural proteins of primate type-D retroviruses.

Colcher¹,

Teramoto²,

Schlom³

1977

Proc. Natl. Acad. Sci. U.S.A.

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A competition radioimmunoassay has been developed in which type-D retroviruses from three primate species compete. The assay utilizes the major structural protein (36,000 daltons) of the endogenous squirrel monkey retrovirus and antisera directed against the major structural protein (27,000 daltons) of the Mason-Pfizer monkey virus isolated from rhesus monkeys. Purified preparations of both viruses grown in heterologous cells, as well as extracts of heterologous cells infected with squirrel monkey retrovirus or Mason-Pfizer monkey virus, compete completely in the assay. Addition of an endogenous virus of the langur monkey also results in complete blocking. No blocking in the assay is observed with type-C baboon viruses, woolly monkey virus, and gibbon virus. Various other type-C and type-B viruses also showed no reactivity. An interspecies assay has thus been developed that recognizes the type-D retroviruses from both Old World monkey (rhesus and langur) and New World monkey (squirrel) species.

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“…Genetically transmitted retroviruses have previously been isolated from both New World and Old World monkeys (1)(2)(3)(4)(5)(6)(7)(8)(9). These isolates have been classified as type C or type D viruses based on morphologic properties and can be subdivided into several distinct classes according to both antigenic and nucleic acid hybridization criteria (8,10).…”

mentioning

confidence: 99%

“…These isolates have been classified as type C or type D viruses based on morphologic properties and can be subdivided into several distinct classes according to both antigenic and nucleic acid hybridization criteria (8,10). Endogenous type C viruses have thus far been isolated from the New World species owl monkey (Aotus trivirgatus) (7) and from Old World species, including stumptail monkey (Macaca arctoides) (8), rhesus monkey (Macaca mulatta) (9), and baboon (Papio anubis) (1)(2)(3); endogenous type D viruses have been isolated from the New World species squirrel monkey (Saimiri sciureus) (4,5) and the Old World species langur (Presbytis obscurus) (6). To date, there have been no convincing isolations of endogenous type C or type D viruses from higher apes or humans.…”

mentioning

confidence: 99%

A new endogenous primate type C virus isolated from the Old World monkey Colobus polykomos.

Sherwin¹,

Todaro²

1979

Proc. Natl. Acad. Sci. U.S.A.

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A new, genetically transmitted retrovirus has been isolated from the Old World monkey Colobus polykomos. This virus, designated CPC-1, is readily transmitted to both feline and human cells in culture. Nucleic acid hybridization studies reveal that there are 50-70 copies of the CPC-1 genome in colobus cellular DNA. Related virogene sequences can be detected in the DNA of all other Old World monkeys, as well as in the DNA of at least one ape species, the chimpanzee, indicating that this virus has been genetically transmitted in primates for 30-40 million years. CPC-1 is partially related to the type C virus previously isolated from stumptail monkeys (MAC-1). These two viruses have nucleic acid sequence homology, antigenic crossreactivity in their major viral structural protein, and a very similar host range in vitro. CPC-1 and MAC-1 therefore belong to the same class of genetically transmitted primate type C viruses and, as such, represent the first example in primates of analogous endogenous retroviruses isolated from two distantly related species.

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Oncornavirus: Isolation From a Squirrel Monkey ( Saimiri sciureus ) Lung Culture

Cited by 107 publications

References 30 publications

Characterization of Infection and Replication of Mason-Pfizer Monkey Virus in Human Cell Cultures

Characterization of Infection and Replication of Mason-Pfizer Monkey Virus in Human Cell Cultures

Interspecies radioimmunoassay for the major structural proteins of primate type-D retroviruses.

A new endogenous primate type C virus isolated from the Old World monkey Colobus polykomos.

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