Ondansetron but not granisetron affect cell volume regulation and potassium ion transport of glioma cells treated with estramustine phosphate

Behnam‐Motlagh, Parviz; Sandström, Per-Erik; Henriksson, Roger; Grankvist, Kjell

doi:10.1007/s00432-002-0362-1

Cited by 6 publications

(2 citation statements)

References 13 publications

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“…[20][21][22][23][24][25][26][27][28][29] Radiotracer-based Rb 86 uptake is another tool for determining the functional characteristics of Na ϩ ,K ϩ -AT-Pase. 11,30,31 Radioligand binding assays using [ 3 H]-ouabain binding has also been employed to study Na ϩ ,K ϩ -AT-Pase. 8,32,33 Similarly, Rb ϩ influxes via Na ϩ ,K ϩ -ATPase and ouabain-resistant pathways have been studied in human blood lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Development of an HTS Assay for Na⁺, K⁺-ATPase Using Nonradioactive Rubidium Ion Uptake

Gill¹,

Gill²,

Wicks³

et al. 2004

ASSAY and Drug Development Technologies

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A high-throughput screening (HTS) assay was developed for the Na(+),K(+)-ATPase channel in order to study rubidium uptake as a measure of the functional activity and modulation of this exchanger. The assay uses elemental rubidium as a tracer for K(+) ions. Three cell lines were used to study the exchanger, and the assay was performed in a 96-well microtiter plate format. Rb(+) uptake was carried by the CHO-K1 cells at 37 degrees C; the maximum ion influx was at 80 min of incubation of the cell line in the medium containing 5.4 mM RbCl. The cells were incubated in Rb(+) uptake buffer (5.4 mM) and with the pump blocker ouabain for 1, 2, and 3 h, respectively. A complete block of the Rb(+) uptake was observed with a 5 mM concentration of ouabain for all the three time intervals. The ouabain 50% inhibitory concentration (IC(50)) value for CHO-K1 cell line ATPase was observed to be 298 microM after 3 h of incubation. In addition, IC(50) values of 94 and 89 microM were observed at 30 min of incubation, indicating that the protocol shows reproducible results. A Z' factor higher than 0.7 was observed in the assays. These studies extend the profile of Na(+),K(+)-ATPases and demonstrate the feasibility of this HTS assay system to screen for compounds that pharmacologically modulate the function of Na(+),K(+)-ATPase.

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Section: Introductionmentioning

confidence: 99%

Development of an HTS Assay for Na⁺, K⁺-ATPase Using Nonradioactive Rubidium Ion Uptake

Gill¹,

Gill²,

Wicks³

et al. 2004

ASSAY and Drug Development Technologies

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“…This ultimately requires increased K secretion into the urine via ROMK to maintain electroneutrality. Based on in vitro data, this effect on the renal tubules appears specific to ondansetron and is not a characteristic of other selective 5-HT 3 antagonists [8]. …”

Section: Introductionmentioning

confidence: 99%

Assessment of Ondansetron-Associated Hypokalemia in Pediatric Oncology Patients

et al. 2012

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Objectives. Ondansetron is a 5-hydroxytryptamine (5-HT3, serotonin) receptor antagonist used as antiemetic prophylaxis preceding chemotherapy administration. Hypokalemia is a rare complication of ondansetron, which may be underreported due to confounding emesis and chemotherapy-induced tubulopathy. We performed a prospective cohort study to determine if ondansetron caused significant hypokalemia independently as a result of renal potassium wasting. Methods. Twelve patients were recruited, with ten completing the study. Blood and urine samples were collected before and after ondansetron administration in patients admitted for intravenous (IV) hydration and chemotherapy. Dietary histories and IV records were analyzed to calculate sodium and potassium balances. Results. We observed an expected drop in urine osmolality, an increase in urine sodium, but no statistically significant change in sodium or potassium balance before and after ondansetron. Conclusion. Ondansetron does not cause significant potassium wasting in appropriately hydrated and nutritionally replete patients. Careful monitoring of serum potassium is recommended in patients with chronic nutritional or volume status deficiencies receiving this medication.

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A hybrid approach to advancing quantitative prediction of tissue distribution of basic drugs in human

Poulin¹,

Ekins

Theil³

2011

Toxicology and Applied Pharmacology

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Ondansetron but not granisetron affect cell volume regulation and potassium ion transport of glioma cells treated with estramustine phosphate

Cited by 6 publications

References 13 publications

Development of an HTS Assay for Na⁺, K⁺-ATPase Using Nonradioactive Rubidium Ion Uptake

Development of an HTS Assay for Na⁺, K⁺-ATPase Using Nonradioactive Rubidium Ion Uptake

Assessment of Ondansetron-Associated Hypokalemia in Pediatric Oncology Patients

A hybrid approach to advancing quantitative prediction of tissue distribution of basic drugs in human

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Ondansetron but not granisetron affect cell volume regulation and potassium ion transport of glioma cells treated with estramustine phosphate

Cited by 6 publications

References 13 publications

Development of an HTS Assay for Na+, K+-ATPase Using Nonradioactive Rubidium Ion Uptake

Development of an HTS Assay for Na+, K+-ATPase Using Nonradioactive Rubidium Ion Uptake

Assessment of Ondansetron-Associated Hypokalemia in Pediatric Oncology Patients

A hybrid approach to advancing quantitative prediction of tissue distribution of basic drugs in human

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Product

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Development of an HTS Assay for Na⁺, K⁺-ATPase Using Nonradioactive Rubidium Ion Uptake

Development of an HTS Assay for Na⁺, K⁺-ATPase Using Nonradioactive Rubidium Ion Uptake