2015
DOI: 10.1128/jb.02365-14
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One-Carbon Metabolic Pathway Rewiring in Escherichia coli Reveals an Evolutionary Advantage of 10-Formyltetrahydrofolate Synthetase (Fhs) in Survival under Hypoxia

Abstract: bIn cells, N 10 -formyltetrahydrofolate (N 10 -fTHF) is required for formylation of eubacterial/organellar initiator tRNA and purine nucleotide biosynthesis. Biosynthesis of N 10 -fTHF is catalyzed by 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase (FolD) and/or 10-formyltetrahydrofolate synthetase (Fhs). All eubacteria possess FolD, but some possess both FolD and Fhs. However, the reasons for possessing Fhs in addition to FolD have remained unclear. We used Escherichia coli, which naturally lacks… Show more

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Cited by 38 publications
(37 citation statements)
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“…However, in direct tests, carolacton has only been shown to inhibit certain isolates of S. pneumoniae [27] and does not affect the growth of most bacteria, including S. mutans at neutral or higher pH [10]. Resistance mechanisms have evolved such as the alternative enzyme Fhs, which is especially widespread in anaerobic bacteria [28], and the efflux pumps which most bacteria possess [26,27,29]. The strong effect of carolacton on S. mutans biofilm viability was always striking because it only occurs at low pH [30], reflecting the essential role of FolD in acid survival of S. mutans [31].…”
Section: Discussionmentioning
confidence: 99%
“…However, in direct tests, carolacton has only been shown to inhibit certain isolates of S. pneumoniae [27] and does not affect the growth of most bacteria, including S. mutans at neutral or higher pH [10]. Resistance mechanisms have evolved such as the alternative enzyme Fhs, which is especially widespread in anaerobic bacteria [28], and the efflux pumps which most bacteria possess [26,27,29]. The strong effect of carolacton on S. mutans biofilm viability was always striking because it only occurs at low pH [30], reflecting the essential role of FolD in acid survival of S. mutans [31].…”
Section: Discussionmentioning
confidence: 99%
“…1. Genetic engineering can be used to enhance the outcome of bioremediation through changing or rewiring of microorganism metabolic pathways so as to enhance the ability of bacteria in degrading the targeted contaminant [21,22]. Also, modifying bacterial genes and regulatory networks to make them tougher to survive and tolerate high contaminant concentration in soils [23].…”
Section: Microorganisms-related Factorsmentioning
confidence: 99%
“…Other genes that had early, persistent mutations include folD (5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, for thymidine biosynthesis) (50) in population A1; and apt (adenosine phosphoribosyltransferase, for purine salvage) (51) in population A5. A 6-bp insertion upstream of apt was found by generation 500 in population A5 and persisted through generation 2,000; and a frameshift in apt coding sequence was in strain E1-5 ( Table S2 ).…”
Section: Resultsmentioning
confidence: 99%