One-Component Systems that Regulate the Expression of Degradation Pathways for Aromatic Compounds

Durante‐Rodríguez, Gonzalo; Gómez-Álvarez, Helena; Nogales, Juan; Carmona, Manuel; Dı́az, Eduardo

doi:10.1007/978-3-319-20796-4_5-1

Cited by 9 publications

(4 citation statements)

References 180 publications

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“…The mechanisms of regulation of these phenol sensors are not well understood. Aromatic metabolic pathways and related genes (Figure A) in Actinobacteria are in general regulated by numerous transcriptional regulators (TRs) that interact directly with the aromatic compounds and the DNA. , These regulators frequently occur in close genomic context to their primary targets (Figure B). It was demonstrated in Rhodococcus erythropolis CCM2595 that the promoter of the two-component phenol hydroxylase operon ( pheA2 [small subunit] and pheA1 [large subunit]) is activated by the AraC family regulator PheR when it binds to phenol .…”

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confidence: 99%

Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630

2017

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Rhodococcus opacus PD630 is a nonmodel, Gram-positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: (1) six fluorescent reporters for quantifying promoter output, (2) three chemically inducible promoters for variable gene expression, and (3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production.

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confidence: 99%

Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630

2017

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“…BphR1 was reported to act as the transcriptional activator involved in the upregulation of the bph operon promoted by 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA), the product of BphC enzyme activity (Watanabe et al, 2000). Although the involvement of GntR-type regulators in the degradation of aromatic compounds is considered to be rather rare, members of this family were also found in several bph operons (Furukawa and Fujihara, 2008;Durante-Rodríguez et al, 2018); these include negative regulators BphS on the transposon Tn4371 from Ralstonia eutropha A5 (Mouz et al, 1999) and Pseudomonas sp. KKS102 (Ohtsubo et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Biphenyl 2,3-Dioxygenase in Pseudomonas alcaliphila JAB1 Is Both Induced by Phenolics and Monoterpenes and Involved in Their Transformation

et al. 2021

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The involvement of bacterial aromatic ring-hydroxylating dioxygenases (ARHDs) in the degradation of aromatic pollutants, such as polychlorinated biphenyls (PCBs), has been well studied. However, there is considerable speculation as to the origin of this ability. One hypothesis is centered on a connection between the ability to degrade aromatic pollutants and the necessity of soil bacteria to cope with and/or utilize secondary plant metabolites (SPMs). To investigate this connection, we researched the involvement of biphenyl 2,3-dioxygenase (BPDO), an ARHD essential for the degradation of PCBs, in the metabolism of SPMs in the soil bacterium Pseudomonas alcaliphila JAB1, a versatile degrader of PCBs. We demonstrated the ability of the strain JAB1 to transform a variety of SPMs, namely the flavonoids apigenin, flavone, flavanone, naringenin, fisetin, quercetin, morin, and catechin, caffeic acid, trans-cinnamic acid, and the monoterpenes (S)-limonene and (R)-carvone. Of those, the transformation of flavone, flavanone, and (S)-limonene was conditioned by the activity of JAB1-borne BPDO and thus was researched in more detail, and we found evidence for the limonene monooxygenase activity of the BPDO. Furthermore, the bphA gene in the strain JAB1 was demonstrated to be induced by a wide range of SPMs, with monoterpenes being the strongest inducers of the SPMs tested. Thus, our findings contribute to the growing body of evidence that ARHDs not only play a role in the catabolism of aromatic pollutants, but also of natural plant-derived aromatics, and this study supports the hypothesis that ARHDs participate in ecological processes mediated by SPMs.

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“…The genome of strain 15-1 harbors eight members of the CRP/FNR superfamily with closest similarities to NO sensors/regulators, five of which were detected in cells grown under oxic and anoxic conditions (Figure S6). Such regulators control transcription of various genes including some involved in degradation of aromatic compounds [80], denitrification, oxidative stress response [60] and transcriptional regulation [81]. For instance, FnrL mediates transcriptional repression of members of the luxR family [81], to which tdiR belongs [19].…”

Section: Discussionmentioning

confidence: 99%

Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier Magnetospirillum sp. Strain 15-1

et al. 2020

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The facultative denitrifying alphaproteobacterium Magnetospirillum sp. strain 15-1 had been isolated from the hypoxic rhizosphere of a constructed wetland model fed with toluene. This bacterium can catabolize toluene anaerobically but not aerobically. Here, we used strain 15-1 to investigate regulation of expression of the highly oxygen-sensitive glycyl radical enzyme benzylsuccinate synthase, which catalyzes the first step in anaerobic toluene degradation. In cells growing aerobically with benzoate, the addition of toluene resulted in a ~20-fold increased transcription of bssA, encoding for the catalytically active subunit of the enzyme. Under anoxic conditions, bssA mRNA copy numbers were up to 129-fold higher in cells growing with toluene as compared to cells growing with benzoate. Proteomics showed that abundance of benzylsuccinate synthase increased in cells growing anaerobically with toluene. In contrast, peptides of this enzyme were never detected in oxic conditions. These findings show that synthesis of benzylsuccinate synthase was under stringent post-transcriptional control in the presence of oxygen, which is a novel level of regulation for glycyl radical enzymes.

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One-Component Systems that Regulate the Expression of Degradation Pathways for Aromatic Compounds

Cited by 9 publications

References 180 publications

Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630

Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630

Biphenyl 2,3-Dioxygenase in Pseudomonas alcaliphila JAB1 Is Both Induced by Phenolics and Monoterpenes and Involved in Their Transformation

Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading Denitrifier Magnetospirillum sp. Strain 15-1

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