2009
DOI: 10.1083/jcb.200810041
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One-dimensional topography underlies three-dimensional fibrillar cell migration

Abstract: Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar extracellular matrix (ECM). We use a novel micropatterning technique termed microphotopatterning (μPP) to identify functions for 1D fibrillar patterns in 3D cell migration. In striking contrast to 2D, cell migration in both 1D and 3D is rapid, uniaxial, independent of ECM ligand density, and dependent on myosin II con… Show more

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Cited by 676 publications
(804 citation statements)
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References 26 publications
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“…While pointing at the different roles exerted by the two Hippo effectors on materials with distinct compliances, these results can also be explained by the different nature of 2D and 3D migration, the latter relying on MMP activation. 44,45 As such, these results would confirm the pivotal function of YAP and TAZ in the switch between mesenchymal and amoeboid migration previously suggested. 46 …”
Section: Articlesupporting
confidence: 89%
“…While pointing at the different roles exerted by the two Hippo effectors on materials with distinct compliances, these results can also be explained by the different nature of 2D and 3D migration, the latter relying on MMP activation. 44,45 As such, these results would confirm the pivotal function of YAP and TAZ in the switch between mesenchymal and amoeboid migration previously suggested. 46 …”
Section: Articlesupporting
confidence: 89%
“…The differences in cell migration speed on FN coating and fibrillar FN matrix are in agreement with other studies on NIH3T3 fibroblasts and human keratinocytes migration in fibrillar FN [20]. Therefore, the increased migration speed cannot be specifically attributed to cancer cells, but it is rather a general phenomenon, which takes place in this type of extracellular environment.…”
Section: Resultssupporting
confidence: 80%
“…For tumor progression, the relevance of cell migration within an ECM network has been highlighted in studies, which report on qualitative and quantitative differences in migration between 2D and 3D environments [20,21]. Regarding the role of ECM proteins in modulating the interactions between MT1-MMP and integrins, surfaces coated with matrix proteins have been shown to differentially regulate the localization and activity of MT1-MMP, α v β 3 -and β 1 -integrins [20].…”
mentioning
confidence: 99%
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“…PVA is a scaffold supporting material for tissue engineering applications. It has been frequently utilized for cell culture attachment (Chen et al 2007;Doyle et al 2009;Michaels et al 1995) and as a hydrogel for bioartificial pancreas (Chuang et al 1999;Qi et al 2004). PVA, as a safe component, has been cleared by the United States Food and Drug Administration (FDA) (Lindemann 1971).…”
Section: Introductionmentioning
confidence: 99%