One-factor-at-a-time (OFAT) optimization of hemicellulases production from<i><i>Fusarium moniliforme</i></i>in submerged fermentation

Kheiralla, Zeinab H.; Ahmed, H. A.; Shaltout, Thanaa H.; Hussein, Mohamed M. D.

doi:10.1080/15567036.2018.1487485

Cited by 11 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the end of fermentation, all mycelia in the shake flask were collected and separated from the broth using gauze, followed by ultrasonic-assisted extraction with ethanol (20:1, mL:g) for 30 min at room temperature. After filtration with filter paper, all filtrate was evaporated in a vacuum at 40 °C to afford a crude extract and subsequently dissolved in One-Factor-At-a-Time (OFAT) design has been the most favored choice for designing fermentation conditions [9]. Response surface methodology (RSM) is an effective mathematical and statistical technique for planning experiments, analyzing variables' effects, creating models, and finding the best combinations of variables for producing adaptive and desired responses [10].…”

Section: Analytical Methods For Samentioning

confidence: 99%

“…One-Factor-At-a-Time (OFAT) design has been the most favored choice for designing fermentation conditions [9]. Response surface methodology (RSM) is an effective mathematical and statistical technique for planning experiments, analyzing variables' effects, creating models, and finding the best combinations of variables for producing adaptive and desired responses [10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Stationary Liquid Fermentation Conditions for N-Methylsansalvamide Production by the Endophytic Strain Fusarium sp. R1

Shao,

Bai,

Cai

et al. 2024

Fermentation

View full text Add to dashboard Cite

To improve the yield of the therapeutic agent N-methylsansalvamide (SA), optimization of stationary liquid fermentation conditions was conducted on an endophytic strain, Fusarium sp. R1, at flask level. Using a One-Factor-At-a-Time approach, the fermentation conditions for SA production were determined as follows: fermentation time of 13 d, 24 °C, initial pH of 6.5, seed age of 24 h, inoculum size of 5.0% (v/v), loading volume of 50% (v/v), and 20.0 g/L salinity. Sucrose, tryptone, and yeast extract were found to be the best sources of carbon and nitrogen. Using response surface methodology, the optimal medium compositions consisted of 22.5 g/L sucrose, 16.5 g/L tryptone, and 0.024 g/L yeast extract. Verification tests suggested that the SA yield under these optimal conditions reached up to 536.77 ± 2.67 mg/L, which was increased by almost ten times the initial yield (54.05 ± 3.45 mg/L). The findings indicate that a high SA production yield can be achieved by stationary culture of strain R1 under proper fermentation conditions using a low-cost medium. This study paves the way toward industrial-scale SA production by strain R1 for new drug development.

show abstract

Section: Analytical Methods For Samentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of Stationary Liquid Fermentation Conditions for N-Methylsansalvamide Production by the Endophytic Strain Fusarium sp. R1

Shao,

Bai,

Cai

et al. 2024

Fermentation

View full text Add to dashboard Cite

show abstract

“…Once the best substrate for SCP production was determined, the fermentation conditions were optimized: pH (3.0-5.5), fermenting temperature (25-40 • C), and time (24-168 h). The conditions were optimized by one factor at a time (OFAT) while keeping the other variables constant [35]. Dry biomass and the crude protein content were determined at each treatment and the results were compared.…”

Section: Optimization Of Fermentation Condition and Comparison With C...mentioning

confidence: 99%

Production of Single-Cell Protein from Fruit Peel Wastes Using Palmyrah Toddy Yeast

et al. 2022

View full text Add to dashboard Cite

Single-cell protein (SCP) from agro-waste material has gained increased attention in the recent past as a relatively cheap and alternative protein source to meet the nutritional demand generated by the fast-growing population. Furthermore, bioconversion of these wastes into SCP such as value-added products reduce the environmental-related issues. In this study, locally available pineapple (Ananas comosus), watermelon (Citrullus lanatus), papaya (Carica papaya), sour orange (Citrus medica), banana (Musa acuminata) and mango (Mangifera indica) peel wastes were investigated for their suitability to produce SCP using palmyrah (Borassus flabellifer) toddy carrying natural mixed yeast and bacteria culture under liquid state fermentation system. Moreover, this study attempted to select the best substrate and the optimized process condition for SCP production to increase the protein yield. The physicochemical properties of selected fruit peels were analyzed. The sterilized peel extracts (10%, v/v) were inoculated with 5 mL of palmyrah toddy and allowed to ferment in a shaking incubator at 100 rpm for 48 h in triplicate at the end of fermentation, the sediments were collected by centrifugation at 1252× g, oven-dried, and the dry weight was taken to determine the protein content. The biomass yield ranged from 5.3 ± 0.6 to 11.7 ± 0.8 g/L, with the least biomass yield being observed with watermelon peels while the maximum yield was observed with papaya peels. Papaya peel generated a significantly higher (p < 0.05) amount of protein (52.4 ± 0.4%) followed by pineapple (49.7 ± 1.3%), watermelon (45.2 ± 0.7%), banana (30.4 ± 0.6%), sour orange (29.5 ± 1.2%) and mango (24.6 ± 0.2%) peels. The optimum condition for the fermentation of papaya waste was pH 5.0, 25 °C, and 24 h. Nucleic acid reduction treatment significantly reduces dry weight and protein content of biomass. It can be concluded that papaya peel waste is a suitable substrate for protein-rich cell biomass production using the natural toddy mixed culture of palmyrah.

show abstract

Efficient Utilization and Bioprocessing of Agro-Industrial Waste

Kumar

Kumari

Dindhoria

et al. 2021

Sustainable Agriculture Reviews 56

View full text Add to dashboard Cite

One-factor-at-a-time (OFAT) optimization of hemicellulases production fromFusarium moniliformein submerged fermentation

Cited by 11 publications

References 15 publications

Optimization of Stationary Liquid Fermentation Conditions for N-Methylsansalvamide Production by the Endophytic Strain Fusarium sp. R1

Optimization of Stationary Liquid Fermentation Conditions for N-Methylsansalvamide Production by the Endophytic Strain Fusarium sp. R1

Production of Single-Cell Protein from Fruit Peel Wastes Using Palmyrah Toddy Yeast

Efficient Utilization and Bioprocessing of Agro-Industrial Waste

Contact Info

Product

Resources

About