2024
DOI: 10.1101/2024.06.17.599441
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One-for-all gene inactivation via PAM-independent base editing in bacteria

Xin Li,
Ying Wei,
Shu-Yan Wang
et al.

Abstract: Base editing is preferable for bacterial gene inactivation without generating double strand breaks, requiring homology recombination or highly efficient DNA delivery capability. However, the potential of base editing is limited by the adjoined dependence on the editing window and protospacer adjacent motif (PAM). Herein, we report an unconstrained base editing system to enable the inactivation of any genes of interests (GOIs) in bacteria. We first employed a dCas9 derivative, dSpRY, as the effector to build a … Show more

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