One health approach on serosurvey of anti-Leptospira spp. in homeless persons and their dogs in South Brazil

Couto, Anahi Chechia do; Gravinatti, Mara Lúcia; Pellizzaro, Maysa; Kmetiuk, Louise Bach; Yamakawa, Ana Carolina; Silva, Evelyn Cristine da; Felipetto, Laís Giuliani; Langoni, Hélio; Leandro, André de Souza; Santi, Carlos Eduardo de; Santos, Andrea Pires dos; Biondo, Alexander Welker

doi:10.1016/j.onehlt.2022.100421

Cited by 6 publications

(7 citation statements)

References 34 publications

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“…Strains were maintained in Ellinghausen-McCullough-Johnson-Harris (EMJH) media at 28 • C, with weekly evaluation and replication. A 1:100 dilution was used as the cutoff point for the serological test, as also established in previous studies [6,15,16]. All samples were analyzed at dilutions ranging from 1:100 to 1:3200, considering the highest positive dilution to possess the serum title [15,17,18].…”

Section: Serology Analysismentioning

confidence: 99%

“…The disease transmission routes have been reportedly associated with socioenvironmental issues including inadequate infrastructure and sewage, garbage accumulation, and rodent proliferation, rendering vulnerable populations in tropical countries as more susceptible to disease [4][5][6][7]. Groups at risk may include gardeners, veterinarians, sewage workers, fishermen, and tourists visiting endemic regions [2].…”

Section: Introductionmentioning

confidence: 99%

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Spatial Owner-Dog Seroprevalence of Leptospira spp. Antibodies in Oceanic Islands and Costal Mainland of Southern Brazil

et al. 2023

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Leptospirosis has been described as a disease neglected worldwide. Affecting humans and animals, the disease is often related to poor environmental conditions such as lack of sanitation and presence of synanthropic rodents. Despite being considered as a One Health issue, no study has focused on comparing owner–dog seroprevalence between islands and seashore mainland. Accordingly, the present study assessed anti-Leptospira spp. antibodies by applying microscopic agglutination test (MAT) methods to Leptospira and assessing associated risk factors via univariate and multivariate logistic regression analysis of owners and their dogs in islands and seashore mainland of southern Brazil. No anti-Leptospira spp. Seropositivity was found in 330 owner serum samples, while dogs presented an overall seroprevalence of 5.9%. All seropositive dogs reacted to serogroups of Leptospira interrogans, including 66.7% of Pyrogenes, 44.4% Canicola, 22.2% Icterohaemorrhagiae, 16.7% Australis; six reacted to more than one serogroup. No association was found among seropositivity and epidemiological variables, except that neighborhood dogs were less likely to be seropositive. Although no seropositivity was observed in owners, seropositivity in dogs had the potential to indicate such species as being sentinels for environmental exposure and potential human risk of infection.

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Section: Serology Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatial Owner-Dog Seroprevalence of Leptospira spp. Antibodies in Oceanic Islands and Costal Mainland of Southern Brazil

et al. 2023

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“…Despite the clinical outcomes, infected animals shed leptospires in the environment, promoting the exposure of other mammals [10,31]. Due to the close proximity to humans, infected dogs play an important role in One Health [32], especially in the cases of asymptomatic harboring [22]. The real impact of canine leptospiral infection and its transmission to humans is poorly understood [32,33].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the close proximity to humans, infected dogs play an important role in One Health [32], especially in the cases of asymptomatic harboring [22]. The real impact of canine leptospiral infection and its transmission to humans is poorly understood [32,33]. Nonetheless, reports show that leptospirosis is endemic in tropical areas, such as Latin American countries [21].…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Pathogenic Leptospira spp. Infecting Dogs in Latin America

Azevedo

Aymée

Borges

et al. 2023

Animals

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Canine leptospirosis is a bacterial disease caused by spirochetes of the genus Leptospira. Infections can vary from asymptomatic and chronic infections to clinical acute diseases. The disease is endemic in tropical areas, such as Latin American countries, but a broad understanding of the dynamics of circulation of strains, based on molecular data, has not yet been performed. Based on in silico analyses, the present study aims to analyze the genetic diversity and circulation patterns of haplotypes from pathogenic leptospires infecting dogs in Latin America. DNA sequences were obtained from GenBank platform, curated, and aligned. Genetic distances were calculated, and a maximum likelihood tree and haplotype network were constructed. According to the inclusion criteria adopted, a total of 148 sequences were identified. Most of the records were from Brazil, including sequences from L. interrogans serogroup Icterohaemorrhagiae. Phylogenetic analysis showed a genetically closely related cluster, consisting of a larger haplogroup that includes the reference strain Fiocruz L1-130, known to be the major circulating strain in humans. Moreover, no genetic variations were observed according to clinical history and/or geographical localization. We described the molecular epidemiology of leptospires circulating among dogs in Latin America and demonstrated a very genetically homogeneous group, elucidating its ubiquitous circulation pattern and drawing attention to the important role of dogs in the One Health transmission dynamics of leptospirosis.

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“…Sua fase aguda é associada à leptospiremia caracterizada por sintomas inespecíficos como fraqueza, tosse, abdominalgia, vômitos e cefaleia (KARPAGAM e GANESH, 2020). No entanto, um dos entraves na diferenciação dessa doença é a sua similaridade com outras doenças febris e sua variada apresentação clínica (COUTO et al, 2022). Ademais, a fase ictérica da doença que ocorre por comprometimento hepático é menos frequente, sendo observada em 5 a 10% dos humanos infectados (KARPAGAM e GANESH, 2020).…”

Section: Introductionunclassified

Ocorrência De Anticorpos Anti-Leptospira Em Humanos E Cães Na Região Sudeste Do Brasil Entre 2018 E 2022: Correlações Epidemiológicas

Rodrigues

Lima

2023

REASE

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A leptospirose é uma doença infectocontagiosa zoonótica causada pela bactéria Leptospira spp. Esta pesquisa objetivou avaliar a prevalência dos sorovares contra Leptospira spp. em humanos e cães na região Sudeste do Brasil durante 2018 a 2022. Para isso, foi feita uma revisão sistemática da literatura. Foram utilizadas as plataformas Scielo, PubMed e Google Acadêmico para a identificação dos artigos que se encaixaram nos requisitos do estudo. Após a identificação dos artigos, foram identificados 11 trabalhos. Na região Sudeste, entre 2018 e 2022, o sorovar mais prevalente em humanos foi o Pyrogenes enquanto em cães foram Icterohaemorrhagiae e Canicola. Além disso, o sorovar que fora identificado em cães e humanos de um mesmo estado foi o Icterohaemorrhagiae. Dessa forma, conclui-se que além de quantificar os casos envolvendo Leptospira spp. em cães e humanos na região Sudeste, foi possível identificar a variedade de sorovares de Leptospira circulantes na região, comuns para as duas espécies.

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One health approach on serosurvey of anti-Leptospira spp. in homeless persons and their dogs in South Brazil

Cited by 6 publications

References 34 publications

Spatial Owner-Dog Seroprevalence of Leptospira spp. Antibodies in Oceanic Islands and Costal Mainland of Southern Brazil

Spatial Owner-Dog Seroprevalence of Leptospira spp. Antibodies in Oceanic Islands and Costal Mainland of Southern Brazil

Molecular Epidemiology of Pathogenic Leptospira spp. Infecting Dogs in Latin America

Ocorrência De Anticorpos Anti-Leptospira Em Humanos E Cães Na Região Sudeste Do Brasil Entre 2018 E 2022: Correlações Epidemiológicas

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