One Health Surveillance of Antimicrobial Resistance Phenotypes in Selected Communities in Thailand

Sudatip, Duangdao; Tiengrim, Surapee; Chasiri, Kittipong; Kritiyakan, Anamika; Phanprasit, Wantanee; Morand, Sergé; Thamlikitkul, Visanu

doi:10.3390/antibiotics11050556

Cited by 5 publications

(5 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MIC values (lowest concentration of antibiotic at which bacterial growth is completely inhibited) of the compounds were determined on three E. coli strains (P53.1R (56), P54.1T (56) and P54.2R (56)), the S. pneumoniae D39 (NCTC 7466) (57) strain, and the S. aureus 20 (56) strain.…”

Section: Resultsmentioning

confidence: 99%

In VitroAntibacterial Activity of Dinuclear Thiolato-Bridged Ruthenium(II)-Arene Compounds

Bugnon

Meléndez

Desiatkina

et al. 2023

Preprint

View full text Add to dashboard Cite

The antibacterial activity of 22 thiolato-bridged dinuclear ruthenium(II)-arene compounds was assessed in vitro against Escherichia coli, Streptococcus pneumoniae and Staphylococcus aureus. None of the compounds efficiently inhibited the growth of the three E. coli strains tested and only compound 5 exhibited a medium activity against this bacterium (MIC (minimum inhibitory concentration) of 25 μM). However, a significant antibacterial activity was observed against S. pneumoniae, with MIC values ranging from 1.3 to 2.6 μM for compounds 1-3, 5 and 6. Similarly, compounds 2, 5-7 and 20-22 had MIC values ranging from 2.5 to 5 μM against S. aureus. The tested diruthenium compounds have a bactericidal effect significantly faster than that of penicillin. Fluorescence microscopy assays performed on S. aureus using the BODIPY-tagged diruthenium complex 15 showed that this type of metal compound enter the bacteria and do not accumulate in the cell wall of gram-positive bacteria. Cellular internalization was further confirmed by inductively coupled plasma mass spectrometry (ICP-MS) experiments. The nature of the substituents anchored on the bridging thiols and the compounds molecular weight appear to significantly influence the antibacterial activity. Thus, if overall a decrease of the bactericidal effect with the increase of compounds' molecular weight is observed, however the complexes bearing larger benzo-fused lactam substituents had low MIC values. This first antibacterial activity screening demonstrated that the thiolato-diruthenium compounds exhibit promising activity against S. aureus and S. pneumoniae and deserve to be considered for further studies.

show abstract

Section: Resultsmentioning

confidence: 99%

In VitroAntibacterial Activity of Dinuclear Thiolato-Bridged Ruthenium(II)-Arene Compounds

Bugnon

Meléndez

Desiatkina

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The MIC values (lowest concentration of antibiotic at which bacterial growth is completely inhibited) of the compounds were determined on three E. coli strains [P53.1R ( 54 ), P54.1T ( 54 ), and P54.2R ( 54 )], the S. pneumoniae D39 (NCTC 7466) ( 55 ) strain, and the S. aureus 20 ( 54 ) strain. Of note, the P53.1R and P54.2R strains were extended-spectrum beta-lactamases resistant, while the P54.1T strain was colistin resistant.…”

Section: Resultsmentioning

confidence: 99%

In vitro antibacterial activity of dinuclear thiolato-bridged ruthenium(II)-arene compounds

Bugnon,

Melendez,

Desiatkina

et al. 2023

Microbiol Spectr

View full text Add to dashboard Cite

The antibacterial activity of 22 thiolato-bridged dinuclear ruthenium(II)-arene compounds was assessed in vitro against Escherichia coli , Streptococcus pneumoniae , and Staphylococcus aureus . None of the compounds efficiently inhibited the growth of the three E. coli strains tested, and only compound 5 exhibited a medium activity against this bacterium [MIC (minimum inhibitory concentration) of 25 µM]. However, a significant antibacterial activity was observed against S. pneumoniae , with MIC values ranging from 1.3 to 2.6 µM for compounds 1–3 , 5, and 6 . Similarly, compounds 2 , 5–7, and 20–22 had MIC values ranging from 2.5 to 5 µM against S. aureus . The tested diruthenium compounds have a bactericidal effect significantly faster than that of penicillin. Fluorescence microscopy assays performed on S. aureus using the BODIPY-tagged diruthenium complex 15 showed that this type of metal compound enters the bacteria and does not accumulate in the cell wall of gram-positive bacteria. Cellular internalization was further confirmed by inductively coupled plasma mass spectrometry experiments. The nature of the substituents anchored on the bridging thiols and the compounds molecular weight appears to significantly influence the antibacterial activity. Thus, if overall a decrease of the bactericidal effect with the increase of compounds’ molecular weight is observed, however, the complexes bearing larger benzo-fused lactam substituents had low MIC values. This first antibacterial activity screening demonstrated that the thiolato-diruthenium compounds exhibit promising activity against S. aureus and S. pneumoniae and deserve to be considered for further studies. IMPORTANCE The in vitro assessment of diruthenium(II)-arene compounds against Escherichia coli , Streptococcus pneumoniae , and Staphylococcus aureus showed a significant antibacterial activity of some compounds against S. pneumoniae , with minimum inhibitory concentration (MIC) values ranging from 1.3 to 2.6 µM, and a medium activity against E. coli , with MIC of 25 µM. The nature of the substituents anchored on the bridging thiols and the compounds molecular weight appear to significantly influence the antibacterial activity. Fluorescence microscopy showed that these ruthenium compounds enter the bacteria and do not accumulate in the cell wall of gram-positive bacteria. These diruthenium(II)-arene compounds exhibit promising activity against S. aureus and S. pneumoniae and deserve to be considered for further studies, especially the compounds bearing larger benzo-fused lactam substituents.

show abstract

“…However, CRE colonization upon admission of such patients could be due to the transmission of CRE from other people or patients who are CRE carriers [ 21 , 22 ]. It is unlikely that these patients would have acquired CRE from the community, since the presence of CRE in the general community in Thailand is still quite low [ 23 , 24 , 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

Active Surveillance for Carbapenem-Resistant Enterobacterales (CRE) Colonization and Clinical Course of CRE Colonization among Hospitalized Patients at a University Hospital in Thailand

et al. 2022

View full text Add to dashboard Cite

Optimal measures for preventing and controlling carbapenem-resistant Enterobacterales (CRE) depend on their burden. This prospective observational study investigated the prevalence and clinical course of CRE colonization in hospitalized patients at Siriraj Hospital, the largest university hospital in Thailand. Stool/rectal swab samples were collected from the patients upon admission, once weekly during hospitalization and every 1–3 months after discharge, to determine the presence of CRE in the stool. Between 2018 and 2021, a total of 528 patients were included. The prevalence of CRE colonization upon admission was 15.5%, while 28.3% of patients who tested negative for CRE on admission acquired CRE during their hospitalization. CRE colonization upon admission was usually associated with prior healthcare exposure. Among CRE-colonized patients, 4.7% developed a CRE clinical infection, with 60% mortality. No cutoff period that ensured that patients were free of CRE colonization in stool was identified, and isolation precautions should only be ceased if stool tests are negative for CRE. In conclusion, the prevalence of CRE colonization among hospitalized patients at Siriraj Hospital is high. CRE-colonized patients are at risk of developing subsequent CRE infection. To prevent CRE transmission within the hospital, patients at high risk of colonization should undergo CRE screening upon admission.

show abstract

One Health Surveillance of Antimicrobial Resistance Phenotypes in Selected Communities in Thailand

Cited by 5 publications

References 22 publications

In VitroAntibacterial Activity of Dinuclear Thiolato-Bridged Ruthenium(II)-Arene Compounds

In VitroAntibacterial Activity of Dinuclear Thiolato-Bridged Ruthenium(II)-Arene Compounds

In vitro antibacterial activity of dinuclear thiolato-bridged ruthenium(II)-arene compounds

Active Surveillance for Carbapenem-Resistant Enterobacterales (CRE) Colonization and Clinical Course of CRE Colonization among Hospitalized Patients at a University Hospital in Thailand

Contact Info

Product

Resources

About