One Orthogonal Phase Microscopy Technique for Orthogonal Phase Imaging of a Cell

Abstract

Abstract. For human beings, the morphological features of cells are closely related to human health thus research into the technique of cell imaging is very important. The optical phase imaging technique is one of the most useful methods because the cell is a phase object. More than one phase images should be needed when rebuilding the 3D sub-structure of a cell. In this paper, in order to obtain the complete 3D information of a cell under the maximum entropy tomography method, two orthogonal phase images is acquired simultaneously, so one orthogonal interference imaging system based on dual orthogonal optical path is constructed by using a lateral displacement beam splitter is proposed. It can obtain two interference images at the same time on the orthogonal direction, so that the biological cellular 3D morphology and its sub-structure can be obtained by using some phase recovery method. Compared with the traditional off-axis interferometry, the full information of the cell is omitted in this method. The simulation and experiment results show the validity and stability of the proposed method. This technique could be widely applied to phase microscopy, especially for transparent samples, such as phase imaging for biological cells and phase measurement, etc.