One-photon excited photoluminescence of gold nanospheres and its application in prostate specific antigen detection via fluorescence correlation spectroscopy (FCS)

Craciun, Ana-Maria; Suarasan, Sorina; Focșan, Monica; Aştilean, Simion

doi:10.1016/j.talanta.2021.122242

“…In the optimal conditions, the linear range of this assay for alphafetoprotein is from 1 pM to 1 nM and the LOD is 1 pM, showing more sensitive, higher selectivity, and faster than current methods (Figure 4b). Additionally, Craciun et al [41] reported a one-step labelfree sensitive homogeneous immunoassay based on gold nanospheres-FCS for the detection of prostate specific antigen (PSA) biomarker (Figure 4c). This approach of monitoring the interaction between anti-PSA-coupled gold and PSA molecules induced gold oligomer diffusion has been successfully validated for detecting PSA levels as low as 4.4 ng mL −1 in solution.…”

Section: Surface-enhanced Raman Scattering Immunoassaymentioning

confidence: 99%

“…In the optimal conditions, the linear range of this assay for alphafetoprotein is from 1 pM to 1 nM and the LOD is 1 pM, showing more sensitive, higher selectivity, and faster than current methods (Figure 4b). Additionally, Craciun et al [41] . reported a one‐step label‐free sensitive homogeneous immunoassay based on gold nanospheres‐FCS for the detection of prostate specific antigen (PSA) biomarker (Figure 4c).…”

Section: Colloidal Gold Labelsmentioning

confidence: 99%

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Advances in nanoprobes‐based immunoassays

Zhang,

Huang,

Jin

et al. 2023

BMEMat

8

0

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Immunoassay is a powerful technique that uses highly specific antigen‐antibody interactions to detect biochemical targets such as proteins and toxins. As a diagnostic tool, immunoassay is employed in the screening, diagnosis, and prognosis of diseases, which are crucial for the grasp and control of patient conditions in clinical practice. With the rapid development of nanotechnology, immunoassays based on nanoprobes have attracted more and more attention due to the advantages of high sensitivity, specificity, stability, and versatility. These nanoprobes are nanoscale particles that can act as signal carriers or targeting agents for immunoassays. In this paper, we review the recent advances in various types of nanoprobes for immunoassays, such as colloidal gold, quantum dots, magnetic nanoparticles, nanozymes, aggregation‐induced emission, and up‐conversion nanoparticles. The effect of the nanoprobe construction and synthesis methods on their detection performance deserves to be studied in depth. We also compare their detection ranges and limits in different immunoassay methods, such as lateral flow immunoassays, fluorescent immunoassays, and surface‐enhanced Raman scattering immunoassays. Moreover, we discuss the benefits and challenges of nanoprobes in immunoassays and provide insights into their future development. This study aims to offer a comprehensive and critical perspective on the role of nanoprobes in the field of immunoassays.

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“…A powerful technique useful for the investigation of photoluminescent nanomaterials is fluorescence lifetime imaging microscopy (FLIM), owing to enhanced sensibility and contrast compared to the conventional steady-state fluorescence imaging. The surpassing imaging quality is gained by mapping the fluorescence lifetime, a parameter that can detect even the smallest structural and environmental changes ( Orte et al, 2013 ; Craciun et al, 2021 ). In our previous work ( Hada et al, 2021b ), we have already demonstrated that BSA-AuNCs can perform as valuable in vitro fluorescence contrast agents for FLIM-based cancer cell imaging, under one-photon excitation (OPE).…”

Section: Introductionmentioning

confidence: 99%

Intrinsic Photoluminescence of Solid-State Gold Nanoclusters: Towards Fluorescence Lifetime Imaging of Tissue-Like Phantoms Under Two-Photon Near-Infrared Excitation

Hada

¹

,

Craciun

²

,

Aştilean

³

2021

Self Cite

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Gold nanoclusters (AuNCs) have attracted extensive attention as light-emissive materials with unique advantages such as high photostability, large Stoke shifts and low toxicity. However, a better understanding of their solid-state photoluminescence properties is still needed. Herein, we investigated for the first time the intrinsic photoluminescence properties of lyophilized bovine serum albumin stabilized AuNCs (BSA-AuNCs) via fluorescence lifetime imaging microscopy (FLIM) studies performed under both one and two photon excitations (OPE and TPE) on individual microflakes, combined with fluorescence spectroscopic investigations. Both in solution and solid-state, the synthesized BSA-AuNCs exhibit photoluminescence in the first biological window with an absolute quantum yield of 6% and high photostability under continuous irradiation. Moreover, under both OPE and TPE conditions, solid BSA-AuNCs samples exhibited a low degree of photobleaching, while FLIM assays prove the homogeneous distribution of the photoluminescence signal inside the microflakes. Finally, we demonstrate the ability of BSA-AuNCs to perform as reliable bright and photostable contrast agents for the visualization of cancer tissue mimicking agarose-phantoms using FLIM approach under non-invasive TPE. Therefore, our results emphasize the great potential of the as synthesized BSA-AuNCs for ex vivo and in vivo non-invasive NIR imaging applications.

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“…A powerful technique useful for the investigation of photoluminescent nanomaterials is uorescence lifetime imaging microscopy (FLIM), owing to enhanced sensibility and contrast compared to the conventional steady-state uorescence imaging. The surpassing imaging quality is gained by mapping the uorescence lifetime, a parameter that can detect even the smallest structural and environmental changes 17,18 . In our previous work 19 , we have already demonstrated that BSA-AuNCs can perform as valuable in vitro uorescence contrast agents for FLIM-based cancer cell imaging, under one-photon excitation (OPE).…”

Section: Introductionmentioning

confidence: 99%

Intrinsic Photoluminescence of Solid-State Gold Nanoclusters: Towards Fluorescence Lifetime Imaging of Tissue-Like Phantoms Under Two-Photon Near-Infrared Excitation

Hada

¹

,

Craciun

²

,

Aştilean

³

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Gold nanoclusters (AuNCs) have attracted extensive attention as light-emissive materials with unique advantages such as high photostability, large Stoke shifts and low toxicity. However, a better understanding of their solid-state photoluminescence properties is still needed. Herein, we investigated for the first time the intrinsic photoluminescence properties of lyophilized bovine serum albumin stabilized AuNCs (BSA-AuNCs) via fluorescence lifetime imaging microscopy (FLIM) studies performed under both one and two photon excitations (OPE and TPE) on individual microflakes, combined with fluorescence spectroscopic investigations. Both in solution and solid-state, the synthesized BSA-AuNCs exhibit photoluminescence in the first biological window with an absolute quantum yield of 6% and high photostability under continuous irradiation. Moreover, under both OPE and TPE conditions, solid BSA-AuNCs samples exhibited a low degree of photobleaching, while FLIM assays prove the homogeneous distribution of the photoluminescence signal inside the microflakes. Finally, we demonstrate the ability of BSA-AuNCs to perform as reliable bright and photostable contrast agents for the visualization of cancer tissue mimicking agarose-phantoms using FLIM approach under non-invasive TPE. Therefore, our results emphasize the great potential of the as synthesized BSA-AuNCs for ex vivo and in vivo non-invasive NIR imaging applications.

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One-photon excited photoluminescence of gold nanospheres and its application in prostate specific antigen detection via fluorescence correlation spectroscopy (FCS)

Cited by 12 publications

References 47 publications

Advances in nanoprobes‐based immunoassays

Advances in nanoprobes‐based immunoassays

Intrinsic Photoluminescence of Solid-State Gold Nanoclusters: Towards Fluorescence Lifetime Imaging of Tissue-Like Phantoms Under Two-Photon Near-Infrared Excitation

Intrinsic Photoluminescence of Solid-State Gold Nanoclusters: Towards Fluorescence Lifetime Imaging of Tissue-Like Phantoms Under Two-Photon Near-Infrared Excitation

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