One-pot biosynthesis of silver nanoparticles with potential antimicrobial and antibiofilm efficiency against otitis media–causing pathogens

Mustafa, Eman A.; Hashem, Abd El Gawad; Elhifnawi, Hala N.; Nada, Hanady G.; Khattab, Rania Abdelmonem

doi:10.1007/s10096-020-03920-w

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…Furthermore, photomicrographs of ultrastructure sections of the A. flavus normal group revealed the regular cellular organelles including nuclei, cell wall, and cell membrane, while using AgNPs led to decreased cell size and deformation of various organelles. This is in the same line as [ 89 ], who reported the beneficial effect of colloidal AgNPs on pathogenic A. flavus . Our results on ultrastructure studies resembled the findings of previous work [ 90 ], which imaged the damage to the integrity of the cell wall and cytoplasmic membrane of Candida albicans through SEM and TEM studies.…”

Section: Resultssupporting

confidence: 88%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC50 = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis.

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Section: Resultssupporting

confidence: 88%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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“…Several factors have been determined for bacterial and fungal ear infections such as swimming, ear picking, headwear, nonsterile oils, steroid usage, malnutrition and immature immunity in children, immunocompromised patient and presence of fungal infection in other part of the body [3]. Emergence of antibiotic resistance is considered a major therapeutic problem [41,42]. In the present research, nine antibiotics (AX, CTX, FOX, TOB, CN, TE, C, CIP, and OFX), belong to five different antibiotic classes namely, penicillins, cephalosporins, aminoglycosides, tetracyclines, chloramphinicols, and quinolones, in addition to a single antifungal agent (NYS) were selected as universally recommended antibiotics by physicians for topical treatments of severe OM infections [43,44].…”

Section: Resultsmentioning

confidence: 99%

In vitro enhancement of ciprofloxacin, tobramycin, and nystatin activity by irradiated aqueous garlic extract against multidrug-resistant pathogens causing otitis media

Nada

Khatab

Hashem

et al. 2021

Arab Journal of Nuclear Sciences and Applications

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Otitis media (OM) is a worldwide major health and economic issue leading to hearing loss, especially in developing countries due to high antibiotic resistance among the causative pathogens. This study aims to isolate, purify and determine the prevalence of MDR microorganisms causing OM. This is in addition to estimate in vitro effectiveness of irradiated and un-irradiated aqueous garlic extract (AGE). Finally, the present study aims to evaluate the enhancement of CIP, TOB, and NYS, commonly used in OM treatment, with IAGE against identified MDR bacteria and fungi. In the current investigation, the in vitro data revealed that OM is more prevalent in middle aged adults than in children. OM were predominated by bacterial isolates (59.0%), followed by fungal isolates (41.0%), including Pseudomonas aeruginosa (5 isolates), Proteus mirabilis (3 isolates), in addition to Klebsiella pneumoniae, Alcaligenes faecalis, Enterococcus faecalis, Bacillus cereus, Penicillium chrysogenum, Aspergillus flavus, and Aspergillus niger (1 isolate). Irradiated AGE (IAGE) showed nearly the same antimicrobial activity as the un-irradiated AGE. The inhibition activity enhancement was more statistically significant (P< 0.001) on combination of IAGE with CIP\NYS than with TOB (P< 0.05) and (P< 0.001) compared with each alone. Noticeable morphological changes in P. aeruginosa and A. flavus were observed by TEM images after the combination of IAGE with CIP/NYS. This study is one of primarily attempts to evaluate the IAGE to be used in future as a natural, safe, sterile, low cost, and available whole additive in therapeutic protocols to overcome MDR problem

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“…53 Combining silver nanoparticles and tea extracts against microbes has drawn attention within the past few years. 50,55,56 The antimicrobial activity of green-synthesized AgNPs (including natural extracts) is described in the recent review by Xin et al 57 In this paper, we propose a method to reduce the working concentration of AgNPs, owing to their synthesis with natural extracts. We synthesized AgNPs with natural extracts from three different types of teablack, green, and red (B-TeaNPs, G-TeaNPs, and R-TeaNPs) and tested them against multidrugresistant pathogenic bacteria and yeasts.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancing the antimicrobial activity of silver nanoparticles against ESKAPE bacteria and emerging fungal pathogens by using tea extracts

Raza,

Wdowiak,

Grotek

et al. 2023

Nanoscale Adv.

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One-pot biosynthesis of silver nanoparticles with potential antimicrobial and antibiofilm efficiency against otitis media–causing pathogens

Cited by 12 publications

References 31 publications

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

In vitro enhancement of ciprofloxacin, tobramycin, and nystatin activity by irradiated aqueous garlic extract against multidrug-resistant pathogens causing otitis media

Enhancing the antimicrobial activity of silver nanoparticles against ESKAPE bacteria and emerging fungal pathogens by using tea extracts

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