One-pot ionic liquid pretreatment and saccharification of switchgrass

Shi, Jian; Gladden, John M.; Sathitsuksanoh, Noppadon; Kambam, Pavan Kumar Reddy; Sandoval, L.A.; Mitra, Debjani; Zhang, Sonny; George, Anthe; Singer, Steven W.; Simmons, Blake A.; Singh, Seema

doi:10.1039/c3gc40545a

Cited by 183 publications

(166 citation statements)

References 46 publications

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“…Imidazolium-and halogen-based ILs were reported to decrease the cellulase activity (Bose et al 2012;Li et al 2013). Even trace amounts of these ILs may worsen significantly the cellulase activity (Zhao et al 2009) and this makes an extensive removal necessary for the residual IL before enzymatic conversion (Datta et al 2010;Shi et al 2013). This seems to be not the case in the experiments in the present paper, where only a simple regeneration and washing processes was applied.…”

Section: Effect Of Aail/cosolvent Pretreatment On Enzymatic Hydrolysiscontrasting

confidence: 47%

Novel cellulose pretreatment solvent: phosphonium-based amino acid ionic liquid/cosolvent for enhanced enzymatic hydrolysis

et al. 2016

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Abstract:The potential of halogen-free and imidazoliumfree phosphonium-based amino acid ionic liquids (AAILs) has been investigated as new solvents for cellulose pretreatment for the subsequent enzymatic hydrolysis of cellulose. AAILs alone did not dissolve cellulose (Avicel), even at 120°C. However, when polar solvents such as dimethylsulfoxide (DMSO) were added as cosolvents, AAILs became an acceptable solvent for cellulose at 30°C. The solubility of cellulose in tetrabutylphosphonium glycine ([TBP][Gly])/cosolvent reached 15%. The enzymatic hydrolysis of cellulose was dramatically enhanced by pretreatment with AAIL/cosolvent, and the glucose yield reached 100% when the novel AAIL tetrabutylphosphonium N,N-dimethylglycine ([TBP][DMGly]) was used in combination with DMSO as cosolvent. The enzymatic conversion of cellulose to glucose in 6% and 13% [TBP] [DMGly]/DMSO buffer solutions reached 98% and 79%, respectively. The decrease in cellulase activity owing to residual [TBP][DMGly]/DMSO was not significant. Hence, it is possible to conduct the dissolution and enzymatic hydrolysis of cellulose in a one-batch process in a phosphonium-based AAIL/cosolvent system.

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Section: Effect Of Aail/cosolvent Pretreatment On Enzymatic Hydrolysiscontrasting

confidence: 47%

Novel cellulose pretreatment solvent: phosphonium-based amino acid ionic liquid/cosolvent for enhanced enzymatic hydrolysis

et al. 2016

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“…Tolerance to this particular IL is of increasing interest as it is currently one of the most effective and well-studied ILs for pretreatment of lignocellulosic biomass [22]. Recent efforts to develop IL-tolerant cellulase cocktails and to incorporate these cocktails into one-pot pretreatment and saccharification bioprocessing schemes show that IL-tolerant enzymes can be used to develop new technologies to deconstruct biomass, and open up the technological landscape for lignocellulosic biorefineries [18].…”

Section: Discussionmentioning

confidence: 99%

“…Fortunately, it has been shown that certain thermophilic bacterial cellulase enzymes can tolerate high levels of the IL [C2mim] [OA]], and in fact these enzymes have been used to develop an IL-tolerant cellulase cocktail called JTherm [13][14][15][16][17]. It has been further demonstrated that JTherm can be used in a one-pot IL pretreatment and saccharification bioprocessing scheme that eliminates the need to wash the pretreated biomass with water, significantly reducing the number of process steps [18].…”

Section: Introductionmentioning

confidence: 99%

Microbial Community Structures Differentiated in a Single- Chamber Air-Cathode Microbial Fuel Cell Fueled with Rice Straw Hydrolysate

Serrano‐Ruiz¹

2015

New Microbial Technologies for Advanced Biofuels

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Background: The development of advanced biofuels from lignocellulosic biomass will require the use of both efficient pretreatment methods and new biomass-deconstructing enzyme cocktails to generate sugars from lignocellulosic substrates. Certain ionic liquids (ILs) have emerged as a promising class of compounds for biomass pretreatment and have been demonstrated to reduce the recalcitrance of biomass for enzymatic hydrolysis. However, current commercial cellulase cocktails are strongly inhibited by most of the ILs that are effective biomass pretreatment solvents. Fortunately, recent research has shown that IL-tolerant cocktails can be formulated and are functional on lignocellulosic biomass. This study sought to expand the list of known IL-tolerant cellulases to further enable IL-tolerant cocktail development by developing a combined in vitro/in vivo screening pipeline for metagenome-derived genes. Results: Thirty-seven predicted cellulases derived from a thermophilic switchgrass-adapted microbial community were screened in this study. Eighteen of the twenty-one enzymes that expressed well in E. coli were active in the presence of the IL 1-ethyl-3-methylimidazolium acetate ([C 2 mim][OAc]) concentrations of at least 10% (v/v), with several retaining activity in the presence of 40% (v/v), which is currently the highest reported tolerance to [C 2 mim][OAc] for any cellulase. In addition, the optimum temperatures of the enzymes ranged from 45 to 95°C and the pH optimum ranged from 5.5 to 7.5, indicating these enzymes can be used to construct cellulase cocktails that function under a broad range of temperature, pH and IL concentrations.

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“…Physically, sugarcane biomass can be divided into four major fractions, whose content depends on the industrial process: fiber (heterogeneous organic solid fraction), nonsoluble solids (inorganic substances), soluble solids (sucrose, waxes and other chemicals), and water (Shi et al, 2013, Canilha et al, 2012. The production of second generation biofuels is focused on using the fiber fraction, especially the cell-wall constituents of the plant, to produce biofuels (Schubert, 2006, Henry, 2010a.…”

Section: Sugarcane Cell-wall and Biomass Compositionmentioning

confidence: 99%

“…Currently available physical and chemical pre-treatment methods are varied and can be listed as un-catalysed steam explosion, flow-through acid, liquid hot water, pH controlled hot water, dilute acid, ammonia and lime, and more recently, the method using ionic liquids (Mosier et al, 2005, Shi et al, 2013. Genetic approaches involve genetic enhancement, molecular biology and plant breeding efforts to improve biomass sources by having crops with less lignin, modified lignin, crops that self-produce enzymes, and crops with increased cellulose and biomass overall (reviewed in Sticklen, 2006).…”

Section: Dealing With the Conversion Issuesmentioning

confidence: 99%

Analysis of genes controlling biomass traits in the genome of sugarcane (Saccharum spp. hybrids)

Hoang¹

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Sugarcane (Saccharum spp. hybrids) is a leading industrial crop in tropical and subtropical regions worldwide. More recently, sugarcane has been selected as a key feedstock for biofuels due to its rapid growth, high fiber content and favorable energy input/output ratio.Breeding sugarcane varieties with biomass for efficient conversion to biofuels can be optimized by understanding the genetic control of biomass composition. However, the genetic analysis of these traits is hindered by the genomic complexity, and the limited availability of a reference genome. The aims of this project were: the development of a high-throughput profiling method for rapid screening of the key biomass traits in a sugarcane population; the construction of a new full-length transcriptome reference database; and the identification of transcripts associated with sugar and fiber accumulation in sugarcane.For the screening of genotypes, newly developed predictive models employing nearinfrared (NIR) spectral analysis, coupled with the high performance liquid chromatography (HPLC), were shown to allow high-throughput profiling of major components in the fiber and sugar fractions in sugarcane biomass. Contrasting genotypes of low fiber and high fiber (minimum of ~29% and maximum of 61% total dry biomass) were identified amongst 331 samples from 186 sugarcane genotypes. The population studied exhibited a wide range of fiber/sugar ratio, from 0.4 (as low as that of the typical commercial sugarcane variety) to 2.2 (similar to that of energy-cane). In addition, the lignin content (the central factor in the biomass recalcitrance) ranged from 6 to 14% of the total dry biomass. To aid genotyping, a new sugarcane transcriptome (termed as SUGIT database) was constructed using PacBio full-length isoform sequencing (Iso-Seq), and a cDNA library derived from 22 diverse sugarcane genotypes, of the key tissues (leaf, internode and root), at different developmental stages (from immature to mature). Comparative analysis showed that this new SUGIT database included more full-length transcripts, longer predicted transcripts, and higher average length of the largest 1,000 proteins, compared to a de novo assembly from Illumina RNA-Seq short-read data from the same sample set. The annotation suggested that the majority (~94%) of the SUGIT database was from coding RNAs, while a very small proportion (~2%) could be long non-coding RNAs. About 70-82% of the RNASeq reads from different tissues mapped back to the SUGIT database, suggesting that it represented well the targeted tissues, while about 69% of this database was aligned with the sorghum genome, confirming the high conservation of orthologs in the genic regions of ii the two genomes. Applying the SUGIT database to differential expression analysis (FDR, false discovery rate corrected p-value <0.05), 1,649 transcript isoforms were identified as being differentially expressed between the young and mature tissues in the sugarcane plant, while 555 transcript isoforms were differentially expressed between th...

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One-pot ionic liquid pretreatment and saccharification of switchgrass

Cited by 183 publications

References 46 publications

Novel cellulose pretreatment solvent: phosphonium-based amino acid ionic liquid/cosolvent for enhanced enzymatic hydrolysis

Novel cellulose pretreatment solvent: phosphonium-based amino acid ionic liquid/cosolvent for enhanced enzymatic hydrolysis

Microbial Community Structures Differentiated in a Single- Chamber Air-Cathode Microbial Fuel Cell Fueled with Rice Straw Hydrolysate

Analysis of genes controlling biomass traits in the genome of sugarcane (Saccharum spp. hybrids)

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