One pot preparation of polyurethane‐based GSH‐responsive core‐shell nanogels for controlled drug delivery

Abstract

In this work, we present a new type of glutathione (GSH)-responsive polyurethane-based core-shell nanogels (RS-CS-PUNGs) with hydrophilic methoxypolyethylene glycols (mPEG) shell, which was prepared by a one-pot synthetic method. The obtained RS-CS-PUNGs not only show a good size distribution with the hydrodynamic radii around of 20 nm, but also exhibit good stability in the organic solvent. The results demonstrate that GSH (10 mM) trigger the nanogel swelling and accelerate the loaded drug release in PBS (pH = 7.4). Although the RS-CS-PUNGs loaded with DOX show a slower cellular uptake behavior than the free doxorubicin (DOX), which is likely caused by the controlled drug release property of the nanocarrier, the enhanced cellular uptake fluorescence intensity of RS-CS-PUNGs loaded with DOX is still observed compared to the control group. Both MTT and CCK-8 assay indicate that although an obvious lower initial cytotoxicity is observed compared to free DOX at 24 h postincubation, the cytotoxicity of the RS-CS-PUNGs loaded with DOX is obvious enhanced after treated 72 h, which stayed at the similar level with free DOX. Attributing to the easy preparation progress and GSH-responsive property, RS-CS-PUNGs maybe hold the potential for further application in the field of drug delivery. the intracellular drug delivery efficiency. 23,30-34 Especially, the disulfide crosslinked nanoparticles, which can stably load the Additional Supporting Information may be found in the online version of this article. † These authors have contributed equally to this work.

Biomedical Polyurethanes for Anti-Cancer Drug Delivery Systems: A Brief, Comprehensive Review

“…Cell experiments confirmed that treatment with DOX-loaded PEG-PU(SS)-PEG micelles significantly inhibited the growth of C6 cells compared with that of other groups [ 38 ]. GSH-responsive PUs were also described in other papers [ 44 , 54 ]. Similarly, reduction-sensitive PUs were synthesized using a disulfide-containing PCL as the hydrophobic block and a cystamine-functionalized PEG as the hydrophilic block [ 40 ].…”

“…Biodegradable PU used in PU-DDSs production can be obtained via polyaddition [ 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ] or the polycondensation process [ 69 ]. IPDI, HDI, LDI, CDI, HMDI, and BDI were used as IC components in biodegradable PUs synthesis.…”

A versatile strategy to construct surface functionalized pH/redox dual stimuli‐responsive nanogels for targeted drug delivery

“…It has been revealed that disulfide bond in nanogels could be cleaved by bioreductive GSH and lead to the degradation of nanogels in cells. 40,43 Accordingly, the reductionsensitivity of P(MAA-PMA)-1 nanogels was studied by monitoring the nanogels' size change and the turbidity change over time in response to 10 mM GSH using DLS. As shown in Figure 3a, the relative turbidity of the P(MAA-PMA)-1 nanogels kept decreasing within 6 h either in 10 mM or 20 mM GSH solutions.…”