Husk derived amino modified linear graphene nanocomposites (aLGN) with a diameter range of 80–300 nm and a length range of 100–300 μm were prepared by a modified Hummers method, ammonia treatment, NaBH4 reduction and phenylalanine induced assembly processes, etc. The resulting composites were characterized by transmission electron microscopy (TEM), atomic force microscopy (AFM), scanning electron microscopy (SEM), biological microscope (BM), and X-ray diffraction spectroscopy (XRD), etc. Investigations found that the aLGN can serve as the novel coating of stir bar sorptive extraction (SBSE) technology. By combing this technology with gas chromatography–mass spectrometry (GC-MS), the combined SBSE/GC-MS technology with an aLGN coating can detect seventeen kinds of amino acids of Camellia nitidissima Chi seeds, including Ala, Gly, Thr, Ser, Val, Leu, Ile, Cys, Pro, Met, Asp, Phe, Glu, Lys, Tyr, His, and Arg. Compared to a conventional polydimethylsiloxane (PDMS) coating, an aLGN coating for SBSE exhibited a better thermal desorption performance, better analytes fragmentation depressing efficiencies, higher peak intensities, and superior amino acid discrimination, leading to a practicable and highly distinguishable method for the variable amino acid detection of Camellia nitidissima Chi seeds.