One-pot synthesis of dual-stimuli responsive hybrid PNIPAAm-chitosan microgels

Echeverría, Coro; Soares, Paula I. P.; Robalo, Ana; Pereira, Laura C. J.; Novo, Carlos; Ferreira, I.; Borges, João Paulo

doi:10.1016/j.matdes.2015.07.170

Cited by 39 publications

(25 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it is worth noting that the l -CS- g -PNIPAM-PTX NPs are positively charged due to the residual amino groups on CS backbones. It is well known that PNIPAM NPs collapse upon heating showing a negative response to temperature and this property is favorable for the stimuli-responsive release of anti-tumor drugs (Echeverria et al., 2015 ). In the present study, the evolution with temperature of the measured hydrodynamic diameter ( Figure 1(F) ) indicates the coincident trend.…”

Section: Resultsmentioning

confidence: 99%

“…These factors indicate that CS a perfect material for delivery of therapeutic agents into the body. On the other hand, PNIPAM is widely recognized as a thermal responsive polymer, since the lower critical solution temperature (LCST) of PNIPAM is closer to body temperature (Echeverria et al., 2015 ) and it undergoes a volume transition in water upon heating above 32 °C. Additionally, PNIPAM is biodegradable, therefore, PNIPAM modified CS has been synthesized as the dual-responsive polymer for promising applications in the biomedical field.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

Niu

Bremner

et al. 2018

Drug Delivery

View full text Add to dashboard Cite

Nanoparticles and macromolecular carriers have been widely used to increase the efficacy of chemotherapeutics, largely through passive accumulation provided by their enhanced permeability and retention effect. However, the therapeutic efficacy of nanoscale anticancer drug delivery systems is severely truncated by their low tumor-targetability and inefficient drug release at the target site. Here, the design and development of novel l-peptide functionalized dual-responsive nanoparticles (l-CS-g-PNIPAM-PTX) for active targeting and effective treatment of GRP78-overexpressing human breast cancer in vitro and in vivo are reported. l-CS-g-PNIPAM-PTX NPs have a relative high drug loading (13.5%) and excellent encapsulation efficiency (74.3%) and an average diameter of 275 nm. The release of PTX is slow at pH 7.4 and 25 °C but greatly accelerated at pH 5.0 and 37 °C. MTT assays and confocal experiments showed that the l-CS-g-PNIPAM-PTX NPs possessed high targetability and antitumor activity toward GRP78 overexpressing MDA-MB-231 human breast cancer cells. As expected, l-CS-g-PNIPAM-PTX NPs could effectively treat mice bearing MDA-MB-231 human breast tumor xenografts with little side effects, resulting in complete inhibition of tumor growth and a high survival rate over an experimental period of 60 days. These results indicate that l-peptide-functionalized acid – and thermally activated – PTX prodrug NPs have a great potential for targeted chemotherapy in breast cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

Niu

Bremner

et al. 2018

Drug Delivery

View full text Add to dashboard Cite

show abstract

“…The CP copolymer was prepared by in-situ free-radical-induced polymerization using TEMED as an accelerator and APS as an initiator in an acetic acid solution (Figure 1). The sulfate radical produced by thermal decomposition of the APS initiator caused AM polymerization to create a PAM long chain structure, which further polymerized with chitosan in situ (Huang et al, 2013;Echeverria et al, 2015;Wang and Yu, 2015). CP copolymers were synthesized with a mass ratio of 3.41:1 (PAM:CS).…”

Section: Synthesis and Characterization Of The Cp Copolymermentioning

confidence: 99%

Enhanced Healing and Antimicrobial Efficacy of Chitosan-g-Polyacrylamide in a Rat Model of Gingival Ulcers

Zheng

Yin

et al. 2020

Front. Chem.

View full text Add to dashboard Cite

Patients in dental hospitals often experience oral ulcerative lesions, which lead to pain and affect the patient's quality of life. At present, the goal of treating oral ulcerative lesions with drugs is to reduce inflammation and promote ulcer healing. However, very few antibacterial and hemostatic drugs are designed to be suitable for the microenvironment of gingival ulcers. Based on this, we have designed a natural therapeutic agent for oral ulcerative lesions that meets the various requirements of oral ulcerative lesion medication. The chitosan-g-polyacrylamide (CP) copolymer is composed of chitosan as the main chain and polyacrylamide polymers as the side chains. Antibacterial experiments show that this polymer can effectively inhibit the proliferation of Gram-negative (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus). In vitro cell experiments also show that the CP copolymer is non-toxic, which is conducive to ulcer wound healing. Coagulation experiments prove that the CP copolymer can accelerate blood coagulation to stop bleeding. In experiments using a Wistar rat gingival ulcer model, the CP copolymer significantly promoted ulcer healing and shortened the healing time. These results indicate that the CP copolymer may serve as a potential therapeutic agent for oral ulcerative lesions.

show abstract

“…As deduced from the literature, the evolution of microgel systems advanced toward the development of hybrid systems (organic/inorganic systems). In fact, since the first work of Antonietti et al [19], in which microgels were used as microreactor and “exo-templates” for the controlled growth of gold nanoparticles, the development of hybrid microgels has increased significantly [20,21,22,23,24,25,26,27,28]. This interest is related to the fact that the incorporation of inorganic nanoparticles into polymeric microgels provided additional functionalities to the final system [29,30].…”

Section: Introductionmentioning

confidence: 99%

A Way to Predict Gold Nanoparticles/Polymer Hybrid Microgel Agglomeration Based on Rheological Studies

Echeverría

Mijangos

2019

Nanomaterials

View full text Add to dashboard Cite

In this work, a detailed rheological study of hybrid poly(acrylamide-co-acrylic acid) P(AAm-co-AAc) aqueous microgel dispersions is performed. Our intention is to understand how the presence of gold nanoparticles, AuNP, embedded within the microgel matrix, affects the viscoelastic properties, the colloidal gel structure formation, and the structure recovery after cessation of the deformation of the aqueous microgel dispersions. Frequency sweep experiments confirmed that hybrid microgel dispersions present a gel-like behavior and that the presence of AuNP content within microgel matrix contributes to the elasticity of the microgel dispersions. Strain sweep test confirmed that hybrid microgels aqueous dispersion also form colloidal gel structures that break upon deformation but that can be recovered when the deformation decreases. The fractal analysis performed to hybrid microgels, by applying Shih et al. and Wu and Morbidelli’s scaling theories, evidenced that AuNP significantly affects the colloidal gel structure configuration ending up with the formation of agglomerates or microgel clusters with closer structures in comparison to the reference P(AAm-co-AAc) aqueous microgel dispersions.

show abstract

One-pot synthesis of dual-stimuli responsive hybrid PNIPAAm-chitosan microgels

Cited by 39 publications

References 40 publications

l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

Enhanced Healing and Antimicrobial Efficacy of Chitosan-g-Polyacrylamide in a Rat Model of Gingival Ulcers

A Way to Predict Gold Nanoparticles/Polymer Hybrid Microgel Agglomeration Based on Rheological Studies

Contact Info

Product

Resources

About