One-Pot Synthesis of Epirubicin-Capped Silver Nanoparticles and Their Anticancer Activity against Hep G2 Cells

Abstract

Epirubicin-capped silver nanoparticles (NPs) were synthesized through a one-pot method by using epirubicin as both the functional drug and the reducing agent of Ag+ to Ag0. The preparation process was accomplished in 1 h. In addition, the obtained epirubicin-capped silver nanoparticle was characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and infrared spectroscopy. The results showed that a layer of polymer epirubicin had formed around the silver nanoparticle, which was 30-40 nm in diameter. We further investigated the antitumor activity of the prepared epirubicin-capped silver nanoparticle, and the half maximal inhibitory concentration (IC50) against Hep G2 cells was 1.92 μg/mL, indicating a good antitumor property of the nanoparticle at low dosage.

Plant-Based Synthesis of Silver Nanoparticles Using Aqueous Leaf Extract of Salvia officinalis: Characterization and its Antiplasmodial Activity

“…The spectra of S. officinalis extracts displayed broad and strong absorbance peak at 3248 cm -1 that corresponds to O-H vibration, which could be arising from phenolic compounds or carboxylic group in the extract [58]. The peak spectrum at 2918.9 cm -1 is assigned to the stretching vibration of CH 2 group [59], vibration peak at 1683 cm -1 indicates C=O stretching or C-N bending in the amide group respectively [35]. The spectra peaks of AgNPs at 3895 cm -1 , 3739 cm -1 , 2842 cm -1 , 2312 cm -1 , 2102 cm -1 , 1900 cm -1 , 1683 cm -1 , 1566 cm -1 , 1215 cm -1 and 1006 cm -1 indicate a shift in absorption bands of the phytochemical compounds from aqueous extract of S. officinalis stabilizing it.…”

Silver Nanoparticles as Carriers of Anticancer Drugs for Efficient Target Treatment of Cancer Cells

“…Regarding the latter approach, the transport systems have many advantages over free anticancer substances, which were mentioned previously. In addition to these benefits already listed, there is the fact that silver itself has anticancer activity [2,[70][71][72]. For these reasons and considering that effective cancer treatment continues to be a big challenge nowadays, it is relevant to have updated knowledge about the state of the art of silver nanoparticles coupled with anticancer drugs and if it is in fact a promising approach for cancer treatment.…”

“…The drug epirubicin (Figure 3D) belongs to the anthracycline family of anticancer agents used to treat breast, lung, and liver cancer. It has several hydroxyl groups, presenting thus useful reducing properties [71]. This drug was another example found in the literature, which was assessed for its anticancer effect when in conjugation with AgNPs acting as nanocarriers.…”

“…This drug was another example found in the literature, which was assessed for its anticancer effect when in conjugation with AgNPs acting as nanocarriers. In the work of Ding et al [71] (2019), it was chemically synthesized AgNPs coupled with epirubicin (EPI), by adding the anticancer drug to a boiling solution of silver nitrate, maintained at 120 • C for 1 h. The purification of the nanoconjugate was accomplished by dialysis in ultra-pure water for 48 h (to remove unbound EPI), followed by centrifugation at 10,000× g for 30 min. The as-synthesized AgNPs showed an average diameter of 36 nm.…”

Silver Nanoparticles Addition in Poly(Methyl Methacrylate) Dental Matrix: Topographic and Antimycotic Studies

“…However, there are many concerns about the use of CNTs for clinical purposes [17]. AgNPs are the most used nanomaterial in different commercial products [18,19,20] and they are strong antibacterial [21] and antimycotic agents [22,23] as well as an anticancer tool [24,25] through their plasmonic features [26,27]. The exact mechanism of AgNPs toxicity is still unclear despite much evidence that has suggested it is their ability to release silver ions (Ag + ) [28], stimulating molecular pathways that induce cell death [29].…”