One-pot synthesized organosilica nanospheres for multiplexed fluorescent nanobarcoding and subcellular tracking

Abstract

Multicolor microbeads are widely used in flow cytometry for various cellular and immunoassays. However, they are limited by the large size around one to tens of micrometer. Nanomaterials for multiplexed...

A novel dual-capability naphthalimide-based fluorescent probe for Fe3+ ion detection and lysosomal tracking in living cells

“…Furthermore, the uorescence intensity changes of MNP were independently tested in a wide range of pH (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). With the increase of pH values, the MNP showed a quenching trend, the uorescence intensity was almost entirely quenched at pH > 8.…”

“…In addition, we investigated the uorescence responses of the MNP to Fe 3+ ions in HEPES buffer (EtOH : H 2 O ¼ 5%; v/v) at various pH values. Furthermore, the uorescence intensity changes of MNP were independently tested in a wide range of pH (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). With the increase of pH values, the MNP showed a quenching trend, the uorescence intensity was almost entirely quenched at pH > 8.…”

“…In recent years, subcellular-organelle targeting strategies have evolved signicantly and redened the future development of multifunctional nano drugs and the clinical transformation of precision medicine. [1][2][3] As eukaryotic organelles, lysosomes contain many hydrolases and secretory proteins that are active in the pH range of acidic solution (3.5-5.5). Lysosomes are the terminal degradation chambers of living cells, and are involved in many physiological processes such as metabolism, apoptosis, intracellular transport, and immunologic defense.…”