One Step Assembly of Thin Films of Carbon Nanotubes on Screen Printed Interface for Electrochemical Aptasensing of Breast Cancer Biomarker

Abstract

Thin films of organic moiety functionalized carbon nanotubes (CNTs) from a very well-dispersed aqueous solution were designed on a screen printed transducer surface through a single step directed assembly methodology. Very high density of CNTs was obtained on the screen printed electrode surface, with the formation of a thin and uniform layer on transducer substrate. Functionalized CNTs were characterized by X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and Brunauer–Emmett–Teller (BET) surface area analyzer methodologies, while CNT coated screen printed transducer platform was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The proposed methodology makes use of a minimum amount of CNTs and toxic solvents, and is successfully demonstrated to form thin films over macroscopic areas of screen printed carbon transducer surface. The CNT coated screen printed transducer surface was integrated in the fabrication of electrochemical aptasensors for breast cancer biomarker analysis. This CNT coated platform can be applied to immobilize enzymes, antibodies and DNA in the construction of biosensor for a broad spectrum of applications.

Preliminary study on conjugation of formononetin with multiwalled carbon nanotubes for inducing apoptosis via ROS production in HeLa cells

“…Figure 3B shows that pure MWCNT-COOH and MWCNT-FMN exhibited diffraction peaks in the range of ~25°–30°, which was assigned to the hexagonal graphite structure. 27 The spectrum of FMN showed numerous distinct peaks at 2θ of 7.4°, 9.8°, 14.86°, 16.2°, 22.4°, and 25.86°, indicating that FMN was a highly crystalline drug. The diffraction peaks of the drug could not be detected in the MWCNT-FMN.…”

Effects of Probiotics on Efficiencies of Feed Use and Slaughter Characteristics of 0-8 Week-Age Ac Broilers

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Recent Progress in Nanomaterial-Based Electrochemical Biosensors for Cancer Biomarkers: A Review

“…The response of the sensors depended on the concentration of hCG in the concentration range from 0.01 to 100 ng mL −1 . In another study, conventional screen-printed carbon electrodes were modified with MWCNTs to prepare aptamer sensors for the breast cancer marker mucine (MUC1) [ 76 ]. The aptamer sensors showed an impedimetric response to MUC1 in a range of 0.1–2 U mL −1 ( Figure 6 ).…”