One-step bioengineering of magnetic nanoparticles via a surface diazo transfer/azide–alkyne click reaction sequence

Abstract

We have developed an efficient conversion of amino iron oxides to carbohydrate and protein derived nanoparticles with highly conserved bioactivity through a combination of diazo transfer and azide-alkyne click technology.Magnetic nanoparticles (MNP) based on iron oxides have attracted much attention for their eclectic applications in biotechnology and medicine. These applications include use as magnetic resonance imaging contrast agents, 1 heating mediators for cancer thermotherapy, 2 magnetic force-based gene delivery, 3 and selective separation and detection of biomolecules. 4

HR-MAS NMR Spectroscopy in Material Science

“…[56] HR-MAS NMR has also been used to identify the binding motif of peptides on the Au NP surface. [57] While HR-MAS NMR is typically employed to overcome anisotropy of magnetic susceptibility or residual dipolar interactions, Poito and coworkers [58,59] have reported an interesting set of experiments that demonstrated HR-MAS can be employed to overcome paramagnetic effects present in iron oxide NPs. Through a careful analysis of these iron oxide systems, the surface structure and ligand binding (chelation) configuration was determined, and in several cases were quite different from the standard single point attachment proposed by others.…”

Structure elucidation of nanoparticle-bound organic molecules by 1H NMR

“…Polito et al [64] developed an efficient conversion for carbohydrate-and protein-derived MNPs via a surface diazo transfer/azide-alkyne click chemistry. Well-resolved 1 H MAS NMR signals at around 8.0 ppm for all modified MNPs confirmed the presence of triazole protons.…”

Luminescence of Polyethylene Glycol Coated CdSeTe/ZnS and InP/ZnS Nanoparticles in the Presence of Copper Cations

“…It is irreversible and the resulting linkage is very stable owing to the formation of a triazole ring. This reaction has therefore been successfully used for the surface functionalization of gold, [11,12] silica, [13] and magnetic nanoparticles, [14,15] as well as carbon nanotubes. [16] The use of click chemistry for quantum dot (QD) functionalization could be very promising for the development of bioconjugates dedicated to in vivo applications.…”