One-step biomimetic coprecipitation method to form calcium phosphate and hemoglobin composite nanoparticles for biosensing application

Zhang, Hui; Xu, Jing‐Juan; Chen, Hong‐Yuan

doi:10.1016/j.jelechem.2008.07.030

Cited by 13 publications

(2 citation statements)

References 31 publications

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“…18 Furthermore, CaP shows good performance for the construction of electrochemical biosensors because Ca 2+ and PO 4 3− can be used as promoters to facilitate electron transfer of proteins. 19 In this work, hierarchical CaP containing bimodal interconnected macroporous and mesoporous structures was used as the matrix for proteins immobilization and biosensor construction owing to its high surface area and advantages in mass transport. 20 Here we described an electrochemical flow injection (FIA) system coupled with a electrochemical detector to study the interaction between 2,4-D and catalase immobilized on hierarchical porous calcium phosphate (hp-CaP) (Scheme 1).…”

Section: ■ Introductionmentioning

confidence: 99%

“…Due to its ability to bind proteins by multiple-site without denaturing them, it has also been used as carriers for peptides, for protein immobilization or DNA delivery . Furthermore, CaP shows good performance for the construction of electrochemical biosensors because Ca 2+ and PO 4 3– can be used as promoters to facilitate electron transfer of proteins . In this work, hierarchical CaP containing bimodal interconnected macroporous and mesoporous structures was used as the matrix for proteins immobilization and biosensor construction owing to its high surface area and advantages in mass transport …”

Section: Introductionmentioning

confidence: 99%

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Inhibition of 2,4-Dichlorophenoxyacetic Acid to Catalase Immobilized on Hierarchical Porous Calcium Phosphate: Kinetic Aspect and Electrochemical Biosensor Construction

Liu

Zhong

et al. 2016

J. Phys. Chem. C

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The inhibition mechanism of 2,4-dichlorophenoxyacetic acid (2,4-D) to catalase was studied by a catalase biosensor in a flow-injection analysis (FIA) system. This system provides an ideal sensing platform to electrochemically evaluate the chemical mechanism of enzyme inhibition. Hierarchical porous calcium phosphate (hp-CaP), as a biocompatible nanomaterial, was used to immobilize catalase for repeat use. 2,4-D together with H 2 O 2 was injected into the bioreactor of the immobilized catalase-FIA system during the experimental procedure. The activity of the immobilized catalase was inhibited, which caused a decrease of the catalase-catalyzed H 2 O 2 reduction current. Lineweaver−Burk, Dixon, and Cornish−Bowden plots confirmed that the inhibition of catalase by 2,4-D followed an competitive mechanism with a inhibition constant of 4.78 × 10 −7 M. Based on this inhibition characters, a biosensor for 2,4-D detection was constructed. This biosensor showed a linear range from 0.03 to 3.00 μM with a detection limit of 0.015 μM. It demonstrated good stability, acceptable reproducibility and low cost for 2,4-D screening. It has been successfully applied for the determination of 2,4-D in commercial bean sprouts samples. It is anticipated that a rapid evaluation of the chemical mechanism of catalase inhibition by the FIA system could pave the way to rationally designing biosensors.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of 2,4-Dichlorophenoxyacetic Acid to Catalase Immobilized on Hierarchical Porous Calcium Phosphate: Kinetic Aspect and Electrochemical Biosensor Construction

Liu

Zhong

et al. 2016

J. Phys. Chem. C

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Direct Electrochemistry of Hemoglobin in Layer‐by‐Layer Films Assembled with DNA and Room Temperature Ionic Liquid

2010

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Based on electrostatic interaction and electrodeposition, poly-anionic deoxyribonucleic acid (DNA), room temperature ionic liquid 1-butyl-3-methyl-imidazolium tetrafluoroborate (BMIMBF 4 ), hemoglobin (Hb) and Poly(diallyldimethylammonium chloride) (PDDA) were successfully assembled into Hb/IL/DNA/PDDA layer-by-layer complex films on the surface of ITO electrode. FTIR spectroscopy, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the composite film. The obtained results demonstrated that the Hb molecule in the film kept its native structure and showed its good electrochemical behavior. A pair of well-defined redox peaks of Hb with the formal potentials (E8') of À 0.180 V (vs. SCE) was appeared in phosphate buffer solution (PBS, pH 7.0). The Hb/IL/DNA/PDDA/ITO modified electrode also showed an excellent electrocatalytic behavior to the reduction of hydrogen peroxide (H 2 O 2 ). Therefore, the IL/DNA/PDDA complex film as a novel matrix open up a possibility for further study on the direct electrochemistry of other proteins and the fabrication of the third-generation electrochemical biosensors.

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A Signal‐Amplified Electrochemical Immunosensor Based on Prussian Blue and Pt Hollow Nanospheres as Matrix

Liu

Peng

et al. 2010

Electroanalysis

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Through electrodepositing Prussian blue (PB) and chitosan (CS), then casting Pt hollow nanospheres (HN-Pt) and assembling CA19-9 antibody on the electrode surface, an immunosensor was achieved. A new signal amplification strategy based on PB and HN-Pt toward the electrocatalytic reduction of H 2 O 2 was employed when performing the determination. The resulting immunosensor showed a high sensitivity, broad linear response to carbohydrate antigen 19-9 (CA19-9) in two ranges from 0.5 to 30 and 30 to 240 U mL À1 with a low detection limit of 0.13 U mL À1 (S/N = 3). Moreover, it displayed good reproducibility and stability, and would be potentially attractive for clinical immunoassay of CA19-9.

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One-step biomimetic coprecipitation method to form calcium phosphate and hemoglobin composite nanoparticles for biosensing application

Cited by 13 publications

References 31 publications

Inhibition of 2,4-Dichlorophenoxyacetic Acid to Catalase Immobilized on Hierarchical Porous Calcium Phosphate: Kinetic Aspect and Electrochemical Biosensor Construction

Inhibition of 2,4-Dichlorophenoxyacetic Acid to Catalase Immobilized on Hierarchical Porous Calcium Phosphate: Kinetic Aspect and Electrochemical Biosensor Construction

Direct Electrochemistry of Hemoglobin in Layer‐by‐Layer Films Assembled with DNA and Room Temperature Ionic Liquid

A Signal‐Amplified Electrochemical Immunosensor Based on Prussian Blue and Pt Hollow Nanospheres as Matrix

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