One-step construction of ferritin encapsulation drugs for cancer chemotherapy

Inoue, I.; Chiba, Mayumi; Ito, Kenichiro; Okamatsu, Yoriko; Suga, Yasuhiro; Kitahara, Yoshiro; Nakahara, Yuko; Endo, Yuta; Takahashi, Kazutoshi; Tagami, Uno; Okamoto, Naoya

doi:10.1039/d0nr04019c

Cited by 36 publications

(37 citation statements)

References 49 publications

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“…HFn decomposes into its subunits in an acidic pH environment and reassembles after returning to a neutral pH, which is one of the most commonly used drug loading methods (Kim et al., 2011 ). However, recent studies have found that treatment with acidic pH will irreversibly destroy HFn and form holes on the surface of the nanocage (Inoue et al., 2021 ). Therefore, we chose mild urea treatment, reassembled after gradual removal of urea, and encapsulated the Cur molecules in HFn nanocages.…”

Section: Resultsmentioning

confidence: 99%

Selective delivery of curcumin to breast cancer cells by self-targeting apoferritin nanocages with pH-responsive and low toxicity

Wang

Yin

et al. 2022

Drug Delivery

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Breast cancer is prevalent and diverse with significantly high incidence and mortality rates. Curcumin (Cur), a polyphenol component of turmeric, has been widely recognized as having strong anti-breast cancer activity. However, its anti-cancer efficiency is largely impaired by some of its concomitant negative properties, including its poor solubility, low cellular uptake, and severe reported side effects. Hence, the necessity arises to develop a novel low-toxic and high-efficiency targeting drug delivery system (DDS). In this study, we developed a pH-sensitive tumor self-targeting DDS (Cur@HFn) based on self-assembled HFn loaded with Cur, in which Cur was encapsulated into HFn cavity by using a disassembly/reassembly strategy, and the Cur@HFn was characterized by ultraviolet–visible (UV–vis), dynamic light scattering (DLS), and transmission electron microscope (TEM). A variety of breast cancer cell models were built to evaluate cytotoxicity, apoptosis, targeting properties, and uptake mechanism of the Cur@HFn. The pharmacodynamics was also evaluated in tumor (4T1) bearing mice after intravenous injection. In vitro release experiments showed that Cur@HFn is pH sensitive and shows sustained drug release under slightly acidic conditions. Compared with Cur, Cur@HFn has stronger cytotoxicity, cellular uptake, and targeting performance. Our study supported that Cur@HFn has a higher in vivo therapeutic effect and lower systemic toxicity. The safety evaluation results indicated that Cur@HFn has no hematotoxicity, hepatotoxicity, and nephrotoxicity. The findings of the present study showed that the Cur@HFn has been successfully prepared and has potential application value in the treatment of breast cancer.

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Section: Resultsmentioning

confidence: 99%

Selective delivery of curcumin to breast cancer cells by self-targeting apoferritin nanocages with pH-responsive and low toxicity

Wang

Yin

et al. 2022

Drug Delivery

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“…Such as methods that target TFR1, which is commonly overexpressed in tumor cell species. W. Liu et al developed endogenous human ferritin heavy-chain nanocages (HFn) to serve as the carrier of paclitaxel (PTX), which can specifically bind to blood-brain barrier and TFR1 widely overexpressed in glioma cells, and showed that HFn-PTX showed the best antitumor effect, and the median survival time was significantly longer than that of free PTX (207); additionally, doxorubicinloaded ferritin heavy chain (DOX-FTH) can be taken up and induce apoptosis of cancer cells overexpressing TFR1 (208); and Cheng et al indicated that HFn-Dox treatment could significantly improve the therapeutic effect of doxorubicin on gastric cancer, and increase overall survival rate of tumorbearing mice (179).…”

Section: Ferroptosis Inducersmentioning

confidence: 99%

The Role of Iron in Cancer Progression

et al. 2021

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Iron is an essential trace element for the human body, and its deficiency or excess can induce a variety of biological processes. Plenty of evidences have shown that iron metabolism is closely related to the occurrence and development of tumors. In addition, iron plays an important role in cell death, which is very important for the development of potential strategies for tumor treatment. Here, we reviewed the latest research about iron metabolism disorders in various types of tumors, the functions and properties of iron in ferroptosis and ferritinophagy, and new opportunities for iron-based on treatment methods for tumors, providing more information regarding the prevention and treatment of tumors.

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“…In addition to DOX, this new method successfully introduced more than a dozen drug molecules (metformin, nicotinamide, creatinine, famotidine, rhodamine B, etc.) into the ferritin nanocage [73]. The development of new drug loading methods is expected to promote the clinical transformation of ferritin nano-drug delivery systems.…”

Section: Ferritin Nanoparticles Combined With Photothermal Therapy (Ptt)mentioning

confidence: 99%

Bioengineered Ferritin Nanocarriers for Cancer Therapy

Sun

Hong

Gong

et al. 2021

IJMS

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Ferritin naturally exists in most organisms and can specifically recognize the transferrin 1 receptor (TfR1), which is generally highly expressed on various types of tumor cells. The pH dependent reversible assembling and disassembling property of ferritin renders it as a suitable candidate for encapsulating a variety of anticancer drugs and imaging probes. Ferritins external surface is chemically and genetically modifiable which can serve as attachment site for tumor specific targeting peptides or moieties. Moreover, the biological origin of these protein cages makes it a biocompatible nanocarrier that stabilizes and protects the enclosed particles from the external environment without provoking any toxic or immunogenic responses. Recent studies, further establish ferritin as a multifunctional nanocarrier for targeted cancer chemotherapy and phototherapy. In this review, we introduce the favorable characteristics of ferritin drug carriers, the specific targeted surface modification and a multifunctional nanocarriers combined chemotherapy with phototherapy for tumor treatment. Taken together, ferritin is a potential ideal base of engineered nanoparticles for tumor therapy and still needs to explore more on its way.

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One-step construction of ferritin encapsulation drugs for cancer chemotherapy

Abstract: A simple preparation of high drug-loaded ferritin nanomedicines was demonstrated. By mixing ferritin and a drug, an electrostatic potential with a small ferritin cavity facilitated the passage and the deposition of the drug in the cavity.

Cited by 36 publications

References 49 publications

Selective delivery of curcumin to breast cancer cells by self-targeting apoferritin nanocages with pH-responsive and low toxicity

Selective delivery of curcumin to breast cancer cells by self-targeting apoferritin nanocages with pH-responsive and low toxicity

The Role of Iron in Cancer Progression

Bioengineered Ferritin Nanocarriers for Cancer Therapy

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