One-Step Electrodeposited Porous ZnO Thin Film Based Immunosensor for Detection of<i>Vibrio cholerae</i>Toxin

Gupta, Pramod K.; Khan, Zishan H.; Solanki, Pratima R.

doi:10.1149/2.0551607jes

Cited by 31 publications

(20 citation statements)

References 39 publications

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“…Both immunosensors gave satisfactory LoD with the GCE-PAN-Ab-BSA giving the least LoD of 3.2 × 10 −13 g / mL compared to that of the GCE-PAN@FePc-Ab-BSA of 2.0 × 10 −12 g/mL. As compared in Table 2 , only few reports gave lower LoD of ~ 10 −15 g/mL [ 10 ] and ~ 10 −17 g/mL [ 15 ], while the LoDs obtained in this work are comparable or even better than some reports [ 9 , 11 – 14 ].…”

Section: Resultssupporting

confidence: 75%

“…Surprisingly, this finding has rarely been reported in any BSA-incorporated electrochemical immunosensors. For example, Gupta et al [ 11 ] observed higher electron transfer rate constants (high-rate constant) with ITO-ZnO-Ab-BSA immunosensor than the corresponding electrodes without BSA (i.e., ITO-ZnO or ITO-ZnO-Ab) and attributed the finding only to the porous nature of the electrode surface. Similar to our case, PAN@FePc is more porous than the PAN (see BET data in Table 1 ), but we believe that the redox kinetics of BSA in the presence of FePc is beyond the porous nature of the PAN@FePc electrode.…”

Section: Resultsmentioning

confidence: 99%

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Bovine Serum Albumin-Dependent Charge-Transfer Kinetics Controls the Electrochemical Immunosensitive Detection: Vibrio cholerae as a Model Bioanalyte

et al. 2021

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Graphical Abstract This work investigates how bovine serum albumin (BSA), a commonly used protein in the fabrication of electrochemical immunosensors, can impact on the sensitivity of detection when integrated with antibody (Ab) pre-encapsulated with (i) insulating polyacrylonitrile (PAN) fibre (i.e., GCE-PAN-Ab-BSA immunosensor) or (ii) conducting PAN-grafted iron (II) phthalocyanine (FePc) (i.e., GCE-PAN@FePc-Ab-BSA immunosensor), using Vibrio cholerae toxin as a case study bioanalyte. Both immunosensors show different charge-transfer kinetics that strongly impact on their immunosensitive detection. From the electrochemical data, GCE-PAN-Ab-BSA is more insulating with the presence of BSA, while the GCE-PAN@FePc-Ab-BSA is more conducting with BSA. The CV of the GCE-PAN-Ab-BSA is dominated by radial diffusion process, while that of the GCE-PAN@FePc-Ab-BSA is planar diffusion process. The behaviour of GCE-PAN@FePc-Ab-BSA has been associated with the facile coordination of BSA and FePc that permits co-operative charge-transport of the redox probe, while that of the GCE-PAN-Ab-BSA is related to the interaction-induced PAN-BSA insulating state that suppresses charge-transport. As a consequence of these different interaction processes, GCE-PAN-Ab-BSA immunosensor provides higher electroanalytical performance for the detection of Vibrio cholerae toxin (with sensitivity of 16.12 Ω/log [VCT, g/mL] and limit of detection (LoD) of 3.20 × 10 −13 g/mL compared to those of the GCE-PAN@FePc-Ab-BSA (4.16 Ω/log (VCT, g mL −1 ) and 2.00 × 10 −12 g/mL). The study confirms the need for a thorough understanding of the physico-chemistries of the electrode platforms for the construction of immunosensors. Although this work is on immunosensors for cholera infection, it may well apply to other immunosensors.

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Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Bovine Serum Albumin-Dependent Charge-Transfer Kinetics Controls the Electrochemical Immunosensitive Detection: Vibrio cholerae as a Model Bioanalyte

et al. 2021

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“…We found that the magnitude of the current increased with increasing Vc T concentration. This increase in the magnitude of the current revealed the formation of an antigen–Ab complex by probing the features of the interfacial properties; the complex acted as an electron‐transfer accelerating layer for redox species on the immunoelectrode surface . Moreover, this might have been due to the fact that when Vc T interacted with the Ab/PANnf/ITO immunoelectrode, the resulting surface charge of the immunoelectrode became quite different from that of the individual Ab or Vc T. This change in the surface energy was related to the electrochemical response .…”

Section: Resultsmentioning

confidence: 99%

Functionalized polyacrylonitrile‐nanofiber based immunosensor for Vibrio cholerae detection

Gupta

Dhakate

et al. 2016

J of Applied Polymer Sci

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Polyacrylonitrile nanofibers (PANnf's) were electrospun directly onto an indium tin oxide (ITO)‐coated glass substrate. The PANnf/ITO electrode was partially hydrolyzed with an NaOH aqueous solution at ambient temperature to convert the nitrile groups of the PANnf's into carboxyl groups was confirmed by Fourier transform infrared spectroscopy. Furthermore, 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide–N‐hydroxy succinimide chemistry was used to activate the COOH groups of PANnf's for the covalent co‐immobilization of monoclonal antibodies against Vibrio cholerae and bovine serum albumin for V. cholerae toxin detection. Structural, functional, and electrochemical studies of the PANnf/ITO electrode and BSA/Ab/PANnf/ITO immunoelectrode were performed, and found a uniform distribution of nanofibers with diameter of 325 ± 7.7% nm. The electrochemical response studies showed an improved sensing performance of the immunoelectrode with a detection of 6.25–500 ng/mL, a low limit of detection of 0.22 ng/mL, a sensitivity of 90 nA ng−1 mL cm−2, an association constant of 45.2 ng/mL, and a dissociation constant of 8 ng/mL. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44170.

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“…Label-free and single-immunoreaction biosensors have been reported using electrode substrates nanostructured with PPI-AuNP nanocomposite [ 81 ], PANnf’s [ 82 ], ZnO NPs [ 83 ], CNTs [ 84 ] and CNFs [ 85 ] for Vibrio cholerae [ 82 ] and its characteristic toxins [ 81 , 82 , 85 ] or antibodies [ 84 ]. Although all these immunosensors exhibit good analytical characteristics in terms of sensitivity and selectivity, none has been applied to the analysis of real samples.…”

Section: Selected Nanostructures In Electrochemical Biosensing Formentioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

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One-Step Electrodeposited Porous ZnO Thin Film Based Immunosensor for Detection ofVibrio choleraeToxin

Cited by 31 publications

References 39 publications

Bovine Serum Albumin-Dependent Charge-Transfer Kinetics Controls the Electrochemical Immunosensitive Detection: Vibrio cholerae as a Model Bioanalyte

Bovine Serum Albumin-Dependent Charge-Transfer Kinetics Controls the Electrochemical Immunosensitive Detection: Vibrio cholerae as a Model Bioanalyte

Functionalized polyacrylonitrile‐nanofiber based immunosensor for Vibrio cholerae detection

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

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