One-step extraction of human erythrocyte lipids allowing rapid determination of fatty acid composition

Peuchant, Evelyne; Wolff, Robert Lee; Salles, Christine; Jensen, Raymond

doi:10.1016/0003-2697(89)90254-6

Cited by 66 publications

(30 citation statements)

References 11 publications

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“…Total lipids were extracted from plasma with 5 ml of hexane/ isopropanol (3:2, by vol). Plasma fatty acid composition was determined from 2 ml of the lipid extract after transformation into isopropyl esters ( 21 ). The isopropyl esters were separated by capillary gas chromatography, as previously detailed ( 8 ).…”

Section: Plasma Fatty Acidsmentioning

confidence: 99%

Relationship between diet and plasma long-chain n-3 PUFAs in older people: impact of apolipoprotein E genotype

Samieri

Lorrain

Buaud

et al. 2013

Journal of Lipid Research

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( P < 0.0001), alcohol intake ( P = 0.0006), and female gender ( P = 0.02), and decreased with intensive consumption of n-6 oils ( P = 0.02). The positive association between fi sh consumption and plasma DHA was highly signifi cant whatever the APOE genotype ( P < 0.0001) but stronger in APOE4 noncarriers than in carriers ( P = 0.06 for interaction). Plasma DHA increased signifi cantly with age ( P = 0.009) in APOE4 noncarriers only. These fi ndings suggest that dietary habits, gender, and APOE4 genotype should be considered when designing interventions to increase n-3 PUFA blood levels in older people. -Samieri, C., S. Lorrain, B. Buaud, C. Vaysse, C. Berr, E. Peuchant, S. C. Cunnane, and P. Barberger-Gateau. Relationship between diet and plasma long-chain n-3 PUFAs in older people: impact of apolipoprotein E genotype. Long-chain n-3 PUFAs [eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)] could potentially exert a protective effect against brain aging due principally to their vascular, anti-infl ammatory, and neuroprotective properties ( 1 ). Accordingly, several epidemiological studies have observed an inverse association between habitual consumption of long-chain n-3 PUFAs or fi sh, their main dietary source, and risk of cognitive decline or dementia ( 1 ). However, this protective association may be limited to Abbreviations: 3C, Three-City; AD, Alzheimer's disease; ALA, ␣ -linolenic acid; APOE4 , 4 allele of the APOE gene; DHA, docosahexaenoic acid; EPA, eicosapentaenoic acid; FFQ, food frequency questionnaire; HR, hazard ratio; RCT, randomized controlled trial.

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Section: Plasma Fatty Acidsmentioning

confidence: 99%

Relationship between diet and plasma long-chain n-3 PUFAs in older people: impact of apolipoprotein E genotype

Samieri

Lorrain

Buaud

et al. 2013

Journal of Lipid Research

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“…Isopropylic ester preparation of fatty acids and their separation for further analysis by a silica capillary column (CP Sil 88 column; Chrompack, Les Ulis, France) with a Carlo Erba model 5160 gas chromatograph equipped with a flame ionization detector (Carlo Erba, Massy, France) were done as described previously (Peuchant et al, 1989). Fatty acids were identified by retention times obtained from various standards (Sigma) and chromatographed under the same conditions.…”

Section: Fatty Acid Determinationmentioning

confidence: 99%

Reduction of dietary saturated fatty acids correlates with increased plasma lecithin cholesterol acyltransferase activity in humans

Berard¹,

Dabadie

Palos-Pinto³

et al. 2004

Eur J Clin Nutr

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Objective: Increased HDL-cholesterol (HDL-C) concentrations have been associated with lower coronary heart disease risk. On the other hand, dietary fats are known to influence the fatty acid profile of plasma lipids, including phospholipids that are substrates of lecithin cholesterol acyltransferase (LCAT), an important enzyme in HDL metabolism. The purpose of this study was to examine the association between the saturated fatty acid (SFA) intake and LCAT activity. Design: An interventional study was performed in a monk community of 25 men. Setting: A French monk community, South West of France. Subjects and interventions: The basal diet of the study cohort contained SFA in a proportion of 13.5% of their total energy intake (TEI). They were submitted to two experimental isocaloric diets containing either 8.4% of the TEI in SFA (diet A) or 11% (diet B), each lasting 5 weeks. Results: The elevation of SFA in diet B was mainly obtained by decreasing carbohydrates. The only significant difference among total fats between diets A and B was the myristic acid content (0.6 and 1.2% of TEI, respectively). The elevation in SFA in diet B resulted in a significant increase of HDL-C (Po0.04), while plasma apo A-I concentration and LCAT activity both decreased (Po0.02). Conclusion: Altogether, these results are consistent with a negative effect of SFA on reverse cholesterol transport.

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“…The fatty acids and aldehydes were converted to FAME and DMA, respectively, with 1 mL 4 H 2 SO 4 in methanol and 1 mg 17:0 as an internal standard at 100 for 30 min 24 . After the reaction, the tubes were cooled on ice and 4 mL 2 KHCO 3 and 5 mL hexane were added.…”

Section: Fatty Acid and Aldehyde Analysismentioning

confidence: 99%

Preparation of Marine Plasmalogen and Selective Identification of Molecular Species by LC-MS/MS

et al. 2014

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One-step extraction of human erythrocyte lipids allowing rapid determination of fatty acid composition

Cited by 66 publications

References 11 publications

Relationship between diet and plasma long-chain n-3 PUFAs in older people: impact of apolipoprotein E genotype

Relationship between diet and plasma long-chain n-3 PUFAs in older people: impact of apolipoprotein E genotype

Reduction of dietary saturated fatty acids correlates with increased plasma lecithin cholesterol acyltransferase activity in humans

Preparation of Marine Plasmalogen and Selective Identification of Molecular Species by LC-MS/MS

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