One-step fabrication of core–shell structured alginate–PLGA/PLLA microparticles as a novel drug delivery system for water soluble drugs

Lim, Ming Pin; Lee, Wei‐Li; Widjaja, Effendi; Loo, Say Chye Joachim

doi:10.1039/c3bm00175j

Cited by 53 publications

(51 citation statements)

References 39 publications

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“…PLGA and PLLA core shell microspheres also allowed sustained release of bupivacaine in vivo during a 2 week implantation in goat knee [81]. In a slightly different approach, a hydrogel based core sheathed with a polyester shell allowed for improved loading of hydrophilic drugs as well as sustained release [82]. In fact, the presence of the core hydrogel allowed for sustained release, although the thickness of the shell as well as the material used have also revealed significant effects on the cargo release [83].…”

Section: Single Drug Deliverymentioning

confidence: 95%

Core–shell designed scaffolds for drug delivery and tissue engineering

2015

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Section: Single Drug Deliverymentioning

confidence: 95%

Core–shell designed scaffolds for drug delivery and tissue engineering

2015

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“…4C). This is distinctly different than many other delivery systems, which generally have an unavoidable burst release prior [15, 17, 44]. Here, protein release from a loaded vehicle was prevented for over three days in the non-degradable group, strongly supporting the idea that in this system, the majority of protein presentation can only occur after shell degradation and release of the protein laden core.…”

Section: Discussionmentioning

confidence: 69%

Core-shell microparticles for protein sequestration and controlled release of a protein-laden core

2017

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Development of multifunctional biomaterials that sequester, isolate, and redeliver cell-secreted proteins at a specific timepoint may be required to achieve the level of temporal control needed to more fully regulate tissue regeneration and repair. In response, we fabricated core-shell heparin-poly(ethylene-glycol) (PEG) microparticles (MPs) with a degradable PEG-based shell that can temporally control delivery of protein-laden heparin MPs. Core-shell MPs were fabricated via a re-emulsification technique and the number of heparin MPs per PEG-based shell could be tuned by varying the mass of heparin MPs in the precursor PEG phase. When heparin MPs were loaded with bone morphogenetic protein-2 (BMP-2) and then encapsulated into core-shell MPs, degradable core-shell MPs initiated similar C2C12 cell alkaline phosphatase (ALP) activity as the soluble control, while non-degradable core-shell MPs initiated a significantly lower response (85±19% vs. 9.0±4.8% of the soluble control, respectively). Similarly, when degradable core-shell MPs were formed and then loaded with BMP-2, they induced a ~7-fold higher C2C12 ALP activity than the soluble control. As C2C12 ALP activity was enhanced by BMP-2, these studies indicated that degradable core-shell MPs were able to deliver a bioactive, BMP-2-laden heparin MP core. Overall, these dynamic core-shell MPs have the potential to sequester, isolate, and then redeliver proteins attached to a heparin core to initiate a cell response, which could be of great benefit to tissue regeneration applications requiring tight temporal control over protein presentation.

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“…For example, core–shell structures enable the controlled sequential delivery of multiple drugs 33 or delivery of hydrophilic drugs. 34 Toward this end, we constructed core–shell aggregates by exploiting the strong, selective interaction between SpyTag and SpyCatcher (Figure 5a). Red fluorescent particles were functionalized with SpyTag or SpyCatcher and mixed in PBS with 0.005% tween 20 to form covalent core structures.…”

Section: Resultsmentioning

confidence: 99%

Protein-Mediated Colloidal Assembly

2017

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Programmable colloidal assembly enables the creation of mesoscale materials in a bottom-up manner. Although DNA oligonucleotides have been used extensively as the programmable units in this paradigm, proteins, which exhibit more diverse modes of association and function, have not been widely used to direct colloidal assembly. Here we use protein–protein interactions to drive controlled aggregation of polystyrene microparticles, either through reversible coiled-coil interactions or through intermolecular isopeptide linkages. The sizes of the resulting aggregates are tunable and can be controlled by the concentration of immobilized surface proteins. Moreover, particles coated with different protein pairs undergo orthogonal assembly. We demonstrate that aggregates formed by association of coiled-coil proteins, in contrast to those linked by isopeptide bonds, are dispersed by treatment with chemical denaturants or soluble competing proteins. Finally, we show that protein–protein interactions can be used to assemble complex core–shell aggregates. This work illustrates a versatile strategy for engineering colloidal systems for use in materials science and biotechnology.

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One-step fabrication of core–shell structured alginate–PLGA/PLLA microparticles as a novel drug delivery system for water soluble drugs

Cited by 53 publications

References 39 publications

Core–shell designed scaffolds for drug delivery and tissue engineering

Core–shell designed scaffolds for drug delivery and tissue engineering

Core-shell microparticles for protein sequestration and controlled release of a protein-laden core

Protein-Mediated Colloidal Assembly

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