One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids

Siltanen, Christian; Diakatou, Michalitsa; Lowen, Jeremy; Haque, Amranul; Rahimian, Ali; Stybayeva, Gulnaz; Revzin, Alexander

doi:10.1016/j.actbio.2017.01.010

Cited by 80 publications

(94 citation statements)

References 40 publications

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“…Each droplet can be barcoded to identify the droplet and its composition in the readout (Figure b) . However, in particular applications, like the prolonged culture of cells structures, droplet emulsions are not considered the optimal mean, with hydrogel microcapsules representing the alternative (Figure c) . Finally, multistep chemical and biological reactions can take place in single droplets through sequential injection of reagents (Figure d).…”

Section: Microfluidic Production Of Microparticles/dropletsmentioning

confidence: 99%

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Microfluidics for Production of Particles: Mechanism, Methodology, and Applications

Liu

Fontana

Python

et al. 2019

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In the past two decades, microfluidics‐based particle production is widely applied for multiple biological usages. Compared to conventional bulk methods, microfluidic‐assisted particle production shows significant advantages, such as narrower particle size distribution, higher reproducibility, improved encapsulation efficiency, and enhanced scaling‐up potency. Herein, an overview of the recent progress of the microfluidics technology for nano‐, microparticles or droplet fabrication, and their biological applications is provided. For both nano‐, microparticles/droplets, the previously established mechanisms behind particle production via microfluidics and some typical examples during the past five years are discussed. The emerging interdisciplinary technologies based on microfluidics that have produced microparticles or droplets for cellular analysis and artificial cells fabrication are summarized. The potential drawbacks and future perspectives are also briefly discussed.

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Section: Microfluidic Production Of Microparticles/dropletsmentioning

confidence: 99%

“…Copyright 2018, The Authors published by Cold Spring Harbor Laboratory Press, under a Creative Common Attribution 4.0 International License. (c) Reproduced with permission . Copyright 2017, Acta Materialia Inc. published by Elsevier Ltd. (d) Reproduced with permission .…”

Section: Microfluidic Production Of Microparticles/dropletsmentioning

confidence: 99%

Microfluidics for Production of Particles: Mechanism, Methodology, and Applications

Liu

Fontana

Python

et al. 2019

Small

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“…In contrast, the platform presented here is easy to use and can be implemented for a variety of droplet microfluidic (partitioning) or continuous phase microfluidic based experiments. Potential applications of this system include recent work profiling immune repertoires from hundreds of thousands of single cells 13 and combined single-cell transcriptome and epitope profiling 14 in addition to ddPCR 15 , ddMDA 16 , hydrogel microsphere fabrication for 3D cell culture 17,18 , chemical microfluidic gradient generation 19 and microparticle size sorting [20][21][22] . The instrument is comprised of electronic and pneumatic components affixed to a 3D printed frame.…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA-seq of rheumatoid arthritis synovial tissue using low cost microfluidic instrumentation

Stephenson

Donlin

Butler

et al. 2017

Preprint

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Droplet-based single cell RNA-seq has emerged as a powerful technique for massively parallel cellular profiling. While these approaches offer the exciting promise to deconvolute cellular heterogeneity in diseased tissues, the lack of cost-effective, reliable, and user-friendly instrumentation has hindered widespread adoption of droplet microfluidic techniques. To address this, we have developed a microfluidic control instrument that can be easily assembled from 3D printed parts and commercially available components costing approximately $540. We adapted this instrument for massively parallel scRNA-seq and deployed it in a clinical environment to perform single cell transcriptome profiling of disaggregated synovial tissue from a rheumatoid arthritis patient. We sequenced 8,716 single cells from a synovectomy, revealing 16 transcriptomically distinct clusters. These encompass a comprehensive and unbiased characterization of the autoimmune infiltrate, including inflammatory T and NK subsets that contribute to disease biology. Additionally, we identified fibroblast subpopulations that are demarcated via THY1 (CD90) and CD55 expression. Further experiments confirm that these represent synovial fibroblasts residing within the synovial intimal lining and subintimal lining, respectively, each under the influence of differing microenvironments. We envision that this instrument will have broad utility in basic and clinical settings, enabling low-cost and routine application of microfluidic techniques, and in particular single-cell transcriptome profiling.

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“…In the flow-focusing method, polymer-cell suspension passes through a microchannel in which cells are further isolated by two focusing axial streams of encapsulant fluid perpendicular to the mainstream, while in the T junction method, there is just one stream of encapsulant fluid perpendicularly isolating the mainstream of polymer-cell suspension in a T-shaped junction. 181,182 Li et al cocultured and encapsulated animal primary hepatocytes and embryonic mouse fibroblasts with PEGDA [poly(ethylene glycol) diacrylate] using flow-focusing approach; finally, the highly spherical microbeads were micropatterned on a collagen type I substrate. The results have shown a long-term survival and function up to 50 days.…”

Section: Extracellular Matrixmentioning

confidence: 99%

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

Mirdamadi

Kalhori

Zakeri

et al. 2020

Tissue Engineering Part B: Reviews

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Chronic liver diseases affect thousands of lives throughout the world every year. The shortage of liver donors for transplantation has been the main driving force to employ alternative methods such as liver tissue engineering (LTE) in fabricating a three-dimensional transplantable liver tissue or enhancing cell delivery techniques alleviating the need for liver donors. LTE consists of three components, cells, ECM (extracellular matrix), and signaling molecules, which we discuss the first and second. The three most common cell sources used in LTE are human and animal primary hepatocytes, and stem cells for different applications. Two major categories of ECM are used to mimic the microenvironment of these cells, named scaffolds and microbeads. Scaffolds have been made by numerous methods with a wide range of synthetic and natural biomaterials. Cell encapsulation has also been utilized by many polymeric biomaterials. To investigate their functions, many properties have been discussed in the literature, such as biochemical, geometrical, and mechanical properties, in both of these categories. Overall, LTE shows excellent potential in assisting hepatic disorders. However, some challenges exist that prevent the practical use of it clinically, making LTE an ongoing research subject in the scientific society.

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One step fabrication of hydrogel microcapsules with hollow core for assembly and cultivation of hepatocyte spheroids

Cited by 80 publications

References 40 publications

Microfluidics for Production of Particles: Mechanism, Methodology, and Applications

Microfluidics for Production of Particles: Mechanism, Methodology, and Applications

Single-cell RNA-seq of rheumatoid arthritis synovial tissue using low cost microfluidic instrumentation

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

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