One-step fabrication of integrated disposable biosensor based on ADH/NAD+/meldola's blue/graphitized mesoporous carbons/chitosan nanobiocomposite for ethanol detection

Hua, Erhui; Wang, Li; Jing, Xiaoying; Chen, Changtao

doi:10.1016/j.talanta.2013.02.064

Cited by 32 publications

(19 citation statements)

References 46 publications

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“…The analytical parameters of GrPE‐ADH‐NAD + are the following: sensitivity (21±2) μA M −1 , detection limit 56 µM and quantification limit 170 µM (taken as indicated above). The biosensor proposed here allows the successful quantification of ethanol in a very fast, sensitive and easy way with better detection limits than other similar biosensing strategies 30, 31, 62, 63. Even when there are other approaches like the association of Gr, CNTs, polymers, ionic liquids, and cross‐linking agents that present lower detection limits 64, 65, it is important to emphasize that in our case, we obtained an excellent analytical performance with a very simple scheme just by mixing GrPE with ADH/NAD + .…”

Section: Resultsmentioning

confidence: 99%

Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol

et al. 2014

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This work reports the analytical applications of a graphene paste electrode (GrPE) for the quantification of dopamine, ethanol and phenolic compounds. Dopamine was detected by differential pulse voltammetry‐adsorptive stripping with medium exchange at submicromolar levels even in the presence of high excess of ascorbic acid and serotonin. The electrocatalytic activity of graphene towards the oxidation of NADH and the reduction of quinones allowed the sensitive amperometric determination of ethanol and phenols using GrPE modified with alcohol dehydrogenase/NAD+ or polyphenol oxidase, respectively, with successful applications in real samples like alcoholic beverages and tea.

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Section: Resultsmentioning

confidence: 99%

Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol

et al. 2014

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“…In electrochemical biosensor, the NAD þ /NADH redox couple have been used as sensing scheme in various electrodes. However, there are only a few number of electrochemical biosensors based on ADHs for the reason that there is frequent constraint for cofactors regeneration [33]. The high over-potentials are required for the electrochemical oxidation of NADH to the corresponding oxidized form NAD þ at a bare electrode surface because the reaction is highly irreversible; many works have been focused on the development of new efficient electrode materials [34].…”

Section: Properties Of Adhmentioning

confidence: 99%

“…Some conducting polymer-based mediators used in alcohol biosensing include polyaniline, poly(thionine) [31], poly(o-aminophenol)-and poly(o-phenylenediamine), polyethylene glycol, poly(vinyl alcohol), polytyrosine and poly(3,4-ethylenedioxythiophene) (PEDOT). The immobilization of mediators like quinines [33], phenoxazines, Figure 5. Scheme showing the reaction sequence within the enzyme electrode using a mediator.…”

Section: Role Of Mediatorsmentioning

confidence: 99%

Alcohol quantification: recent insights into amperometric enzyme biosensors

Hooda

Kumar

Gahlaut

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Biosensors are the switching channels that make sense. The biosensors have found an empirical role in health applications (e.g. clinical diagnostics) as they represent the technological counterpart of living senses. On a global scale, alcohol analysis is indispensable for criminal justice systems, monitoring medical conditions of HIV patients & pregnant women as well as public safety issues regarding pilots, metro drivers, doctors etc. For addressing the clinical and toxicological problems, much advancement in the improvement of biosensors have been witnessed in recent decades. Currently, electrochemical biosensors dominate the field which harnesses the synergistic action of enzymes and nanomaterials for the analysis of ethanol. The enzymatic biosensors are the most explored biosensing devices among all the types of biosensors, and employment of nanomaterials has paved a way to the further improvements in this gem of a discovery. The relative comparison to precise the alcohol biosensors has been aptly discussed in the review on the basis of several analytical properties including fabrication, linearity, sensitivity, response time, detection limit as well as storage stability. Finally, the recent trends and emerging future prospects of alcohol biosensors have been reviewed.

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“…In most of these studies, chitosan has been used as a film-forming material, not as an active sensing material. In addition to these, chitosan was used in more complex composites for gas sensing [16,17]. Molla-Abbasi and Ghaffarian suggested the use of chitosan-decorated carbon nanotube surfaces for polar chemical vapors [18].…”

Section: Introductionmentioning

confidence: 99%

UV-Enhanced Humidity Sensing of Chitosan–SnO2 Hybrid Nanowires

et al. 2020

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The surface of SnO2 nanowires was functionalized by chitosan for the development of room-temperature conductometric humidity sensors. SnO2 nanowires were synthesized by the seed-mediated physical-vapor-deposition (PVD) method. Chitosan layers were deposited on top of the SnO2 nanowires by spin coating. Surface morphology, crystal structure, and optical properties of the synthesized hybrid nanostructure were investigated by scanning electron microscope, grazing incidence X-ray diffraction, and UV–Vis absorption measurements. During electrical conductivity measurements, the hybrid nanostructure showed unusual behavior towards various relative humidity (RH) concentrations (25%, 50%, 75%), under UV-light irradiation, and in dark conditions. The highest sensor responses were recorded towards an RH level of 75%, resulting in 1.1 in the dark and 2.5 in a UV-irradiated chamber. A novel conduction mechanism of hybrid nanowires is discussed in detail by comparing the sensing performances of chitosan film, SnO2 nanowires, and chitosan@SnO2 hybrid nanostructures.

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One-step fabrication of integrated disposable biosensor based on ADH/NAD+/meldola's blue/graphitized mesoporous carbons/chitosan nanobiocomposite for ethanol detection

Cited by 32 publications

References 46 publications

Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol

Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol

Alcohol quantification: recent insights into amperometric enzyme biosensors

UV-Enhanced Humidity Sensing of Chitosan–SnO2 Hybrid Nanowires

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