One-step glass-like coating of polycarbonate for seamless DNA purification and amplification on an integrated monolithic microdevice

Irreversible bonding techniques for the fabrication of a leakage-free printed circuit board-based lab-on-chip in microfluidic platforms—a review

“…In general, the bonding strength of substrates employing amine-functional and other organofunctional silanes are relatively robust and applicable for high-pressure microfluidic applications. In fact, the mechanical strength results from using this bonding approach is significantly much higher compared with those of the thermal-based methods, as suggested by the results from the recent studies on PDMS substrates [101,107,109,110,113]. On the average, the mechanical strength reported by the authors was approximately 1000 psi.…”

“…In addition to the amino-functional types, other kinds of organofunctional silanes were also explored to bond various types of plastic and Si-containing substrates [111][112][113][115][116][117]. Similar to the methods discussed in this section, bonding that employs organofunctional silanes can also be achieved at temperatures below T g , as evidenced by the previous studies [12-14, 16, 111, 113, 117].…”

Heat and pressure-resistant room temperature irreversible sealing of hybrid PDMS–thermoplastic microfluidic devices via carbon–nitrogen covalent bonding and its application in a continuous-flow polymerase chain reaction

“…† In this procedure, the temperatures of two copper heaters were separately controlled for denaturation and annealing/extension as described in our previous studies. [31][32][33][34] The temperature measured on the surface of the copper heaters was considered to be the temperature inside the microchannel since the PC where the microchannel was engraved was only 2 mm in thickness, and the relatively low thermal conductivity of the PC (0.22 W K À1 m À1 ) prevented heat dissipation and conned heat within the PC. The surface of the PC was then covered with black tape to reduce the heat reection during temperature measurement using an IR camera.…”

An electrophoretic DNA extraction device using a nanofilter for molecular diagnosis of pathogens

“…Gan et al 17 integrated chitosan-modified filter paper in a poly(methyl methacrylate) microfluidic device to develop a low-cost and rapid DNA extraction method. Zhang et al 18 proposed sol-gel coating on a polycarbonate (PC) surface with bis [3-(trimethoxysilyl)propyl]aminosilane for purifying DNA from a crude sample as well as one-step bonding of two PC substrates to fabricate a monolithic microchip. Although the microchip-based DNA separation has been improved in terms of portability and simplicity, DNA recovery yield is still a limitation due to the use of the solid phase DNA extraction mechanism.…”