One step gold (bio)functionalisation based on CS2-amine reaction

Almeida, I.; Cascalheira, A.C.; Viana, Ana S.

doi:10.1016/j.electacta.2010.07.084

Cited by 24 publications

(25 citation statements)

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“…25,26,31,32 Because protein A is completely devoid of tryptophan and contains only few phenylalanine and tyrosine residences in its structure, its UV−vis absorption spectroscopy does not present clear peaks between 260 and 280 nm at low concentrations, 41 which is helpful to observe the appearance of absorption bands of DTC in a similar wavelength range. CS 2 exhibits a characteristic peak near 315 nm, for which the 28,31 or smaller compounds. 25 However, these two characteristic peaks can shift depending on the structure of the side chain.…”

Section: Resultsmentioning

confidence: 99%

“…The method shows compelling potential for ligand immobilization in immunosensors employing gold surface as the substrate. Although there are some reported studies using this methodology, they are mostly focused on small molecules; 25,28−33 for example, dopamine, epinephrine, different kinds of amino acid (e.g., tryptophan), and very few larger molecules, such as polyethyleneimine (22 kDa) 34 and biocompounds, namely, oligonucleotide 35 and glucose oxidase, 28 have been immobilized on gold by this new method. To our knowledge, this is the first study reporting the use of in situ DTC formation to prepare an immunosensing surface and clearly show its feasibility toward ligand detection and prevention of nonspecific adsorption.…”

Section: Introductionmentioning

confidence: 99%

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Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

et al. 2012

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Carbon disulfide (CS 2 ) can spontaneously react with amine groups to form dithiocarbamates on gold surface, providing the possibility to immobilize some compounds with primary or secondary amine groups in one step. Using this principle, an immunosensor interface prepared for immunoglobulin G (IgG) sensing surface toward anti-IgG has been fabricated for the first time by simply immersing gold slides into a mixed aqueous solution of CS 2 and protein A, followed by incubation in immunoglobulin G solution. The reaction between CS 2 and protein A has been followed by UV−vis spectroscopy, whereas cyclic voltammetry has been employed in the characterization of the modified gold surface with CS 2 and protein A, both methods indicating that protein A immobilization is implemented by CS 2 . Conventional ellipsometry, atomic force microscopy (AFM), as well as surface plasmon resonance (SPR) have been used to evaluate the specific binding of protein A with IgG and IgG with anti-IgG, revealing that IgG is specifically captured to form the biosensing interface, maintaining its bioactivity. Compared to direct adsorption of IgG on the gold surface, the IgG sensing surface constructed of CS 2 and protein A is far more sensitive to capture anti-IgG as its target molecule. In addition, the modified surface is proven to have good capability to inhibit nonspecific adsorption, as supported by control experiments using lysozyme and BSA. To conclude, antibody immobilization using this one-step method has potential as a simple and convenient surface modification approach for immunosensor development.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

et al. 2012

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“…For ellipsometry and AFM analysis, gold substrates manufactured by Arrandee consisting of a thin film (200 nm) of gold on borosilicate glass (1.1 × 1.1 cm 2 ) with a chromium undercoat (2-4 nm) were used and cleaned in piranha solution (H 2 SO 4 /H 2 O 2 = 3:1, v/v) and in a UV chamber for 45 min as described elsewhere [5,27]. These latter substrates were also flameannealed after which they acquired a preferential (111) orientation, as proved by scanning tunneling microscopy measurements [28].…”

Section: Preparation Of Gold Surfacesmentioning

confidence: 99%

Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces

Almeida

Marquês

Liu

et al. 2015

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tIn this work, a simple yet robust method to prepare lipid-based biosensing interfaces on gold using common lipids (a phospholipid and cholesterol) and an alkanethiol is reported. The lipids were carefully chosen to tailor the biophysical properties of the bilayer. The simplicity of the method relies on the incorporation of a small percentage of decanethiol in the lipid vesicles for a direct formation of a thiol-linked supported lipid bilayer, which is advantageous in several respects. It prevents the use of specially synthesized thiolipids and preserves the natural fluidity and dynamics of the lipids. As a consequence the whole arrangement is extremely stable regarding ionic strength changes and solution flow during surface plasmon resonance experiments. Moreover, we show that this interface is very effective on suppressing the nonspecific adsorption of proteins on the surface, and enables the covalent attachment of the recognition antibody. The subsequent detection of specific interaction toward antigen was monitored in real-time by SPR and confirmed by ellipsometric measurements. This lipid-based biosensing platform is versatile and can be adapted to the biorecognition reaction of interest.

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“…DTC have a resonant bidentate structure along N-C-S 2 and establish strong linkages to gold substrates [16]. This methodology has been used to immobilize a wide range of molecules and biocompounds [13,[17][18][19][20][21] and, importantly, to prepare immunosensor interfaces for the detection of biological molecules relying on the specific antibody-antigen reaction. Furthermore, the ability to prevent protein nonspecific adsorption to the biorecognition surfaces has been also demonstrated [12].…”

Section: Introductionmentioning

confidence: 99%

“…However, this method requires the incorporation of an activation step, usually performed with N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), to attach the protein to the SAM [8][9][10]. An approach using the in situ formation of dithiocarbamates (DTC) to, in only one-step, immobilize compounds with amine groups to gold surfaces has been developed as an efficient alternative [11][12][13]. Briefly, carbon disulfide spontaneously reacts with amine groups to form DTC through the immersion of gold substrates in a solution containing CS 2 and the compound to immobilize, at room temperature [11,14,15], without the need of adding several modification and activation steps.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Paiva

Almeida

Marquês

et al. 2017

Applied Surface Science

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a b s t r a c tIn this work, we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups, present on protein A, ii) their attachment to gold nanoparticles (AuNPs), and iii) the linkage of the modified AuNPs to the electrode surface, which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry, conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time, by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry, through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection, whereas the presence of co-adsorbed CS 2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall, we report a simple and innovative one-step method, to chemically modify gold surfaces with protein A and AuNPs, able to specifically detect antigen/antibody interactions with capability of preventing protein nonspecific adsorption.

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One step gold (bio)functionalisation based on CS2-amine reaction

Cited by 24 publications

References 50 publications

Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

Phospholipid/cholesterol/decanethiol mixtures for direct assembly of immunosensing interfaces

Nanostructured interfaces with site-specific bioreceptors for immunosensing

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