One step immobilization of peptides and proteins by using modified parylene with formyl groups

Stable nonpolar solvent droplet generation using a poly(dimethylsiloxane) microfluidic channel coated with poly-p-xylylene for a nanoparticle growth

“…These functionalized parylenes can also be used to create reactive groups on the non-reactive PDMS surface. The functionalized surfaces with parylene-H and parylene-A film shows easy and higher antibody immobilized efficiency for a biosensor (Ko et al 2011), cell proliferation (Zhang et al 2011), and other biological applications (Tan and Craighead 2010). These surface modifications of PDMS by parylenes shows that the parylene coating procedure compromises the drawbacks of PDMS and expands the utility of microchannels made of PDMS.…”

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry of small volatile molecules using a parylene‐matrix chip

“…The CHCA matrix solution was prepared at a concentration of 10 mg/mL in acetonitrile/water (1:1, v/v) and 0.1% TFA, and 1 μL per sample spot was dropped on a stainless steel target plate (MSP 96 ground steel target for the microScout) from Bruker Daltonics Co. (Germany). After the matrix spots had dried at room temperature, a parylene‐N film was deposited via thermal evaporation at 160 °C, pyrolysis at 650 °C and deposition at room temperature using a microprocessor‐controlled parylene‐coater from Femto Science (Hwasung, Korea) . The thickness of the parylene‐N film was controlled to be 30 nm by adjusting the amount of parylene‐N precursor, and the thickness was monitored using AFM analysis.…”

Development of a sensitive SPR biosensor for C-reactive protein (CRP) using plasma-treated parylene-N film

“…In this work, the thickness of the parylene-N film was controlled to be 30 nm by adjusting the initial amounts of the parylene-N precursors. The plasma-treatment of the parylene-N films was carried out as previously described using a plasma generator from Femto Science Co. (Seoul, Korea) [10][11][12]. The plasma-treatment time was controlled to be from 1 to 5 min, and the plasma power was set between 10 and 100 W. The initial vacuum was controlled to be less than 0.05 Torr, and then it was controlled to be 0.5-1.0 Torr with the oxygen flow of 50 cc/min.…”

“…As parylene films have strong chemical resistance, good optical properties, and electric isolating property, they have been widely used in various fields [2][3][4]. For the protein immobilization of small peptides and proteins, functionalized parylenes with chemical functional groups such as amine (parylene A) and formyl (parylene H) could be effectively used, because it allows the formation of covalent bonds between the immobilized protein and the functional groups of the surface [6][7][8][9][10][11][12]. Recently, we reported that a plasma-treated parylene-N film could be used effectively for protein immobilization through physical adsorption in comparison with the polystyrene surface [13][14][15][16][17][18][19].…”