One-step immunoassay without washing steps for influenza A virus detection using ISFET

Bong, Ji Hong; Kim, Hong Rae; Yoo, Jae Woo; Kang, Min Jung; Shin, Myung Geun; Lee, Jung Su; Shim, Won Bo; Lee, Sang Dae; Pyun, Jae Chul

doi:10.1016/j.bios.2020.112341

Cited by 15 publications

(8 citation statements)

References 28 publications

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“…In addition, a one-step immunoassay for the detection of influenza A virus was developed by using two different microbead types and a filter-inserted bottle [7]. The detection bead could pass through the filter into the reaction buffer containing urea and induce a pH change, which was quantitatively measured by using phenol red and ISFET.…”

Section: Introductionmentioning

confidence: 99%

Design and Implementation of a pH Sensor for Micro Solution Based on Nanostructured Ion-Sensitive Field-Effect Transistor

Wang

Yang

2020

Sensors

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pH sensors based on a nanostructured ion-sensitive field-effect transistor have characteristics such as fast response, high sensitivity and miniaturization, and they have been widely used in biomedicine, food detection and disease monitoring. However, their performance is affected by many factors, such as gate dielectric material, channel material and channel thickness. In order to obtain a pH sensor with high sensitivity and fast response, it is necessary to determine the appropriate equipment parameters, which have high processing cost and long production time. In this study, a nanostructured ion-sensitive field-effect transistor was developed based on the SILVACO technology computer-aided design (TCAD) simulator. Through experiments, we analyzed the effects of the gate dielectric material, channel material and channel thickness on the electrical characteristics of the nanostructured field-effect transistor. Based on simulation results, silicon nitride was selected as the gate dielectric layer, while indium oxide was chosen as the channel layer. The structure and parameters of the dual channel ion-sensitive field-effect transistor were determined and discussed in detail. Finally, according to the simulation results, a pH sensor based on the nanostructured ion-sensitive field-effect transistor was fabricated. The accuracy of simulation results was verified by measuring the output, transfer and pH characteristics of the device. The fabricated pH sensor had a subthreshold swing as low as 143.19 mV/dec and obtained an actual sensitivity of 88.125 mV/pH. In addition, we also tested the oxidation reaction of hydrogen peroxide catalyzed by horseradish peroxidase, and the sensitivity was up to 144.26 pA mol−1 L−1, verifying that the ion-sensitive field-effect transistor (ISFET) can be used to detect the pH of micro solution, and then combine the enzyme-linked assay to detect the concentration of protein, DNA, biochemical substances, biomarkers, etc.

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Section: Introductionmentioning

confidence: 99%

Design and Implementation of a pH Sensor for Micro Solution Based on Nanostructured Ion-Sensitive Field-Effect Transistor

Wang

Yang

2020

Sensors

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“…11−13 Recently, the performance of a one-step immunoassay based on switching peptides was reported to be effective without any washing step. 3,13 As shown in Figure 1a, the switching peptides specifically bind to the frame regions (FRs) at the binding pocket of antibodies (IgGs), and these peptides are released when antigens specifically bind to the complementarity-determining regions (CDRs) of antibodies.…”

mentioning

confidence: 99%

“…Therefore, the switching peptides labeled with fluorescent dyes were dissociated from IgG as soon as the binding of target analytes (antigens) and the one-step immunoassay without any washing step were possible by the measurement of the fluorescence signal. 3,13 In this study, switching peptides were employed in an electrochemical one-step immunoassay instead of with optic-based transducers. 17−19 Usually, amperometry-based immunoassays have been used for immunoassays using secondary antibodies labeled with enzymes and chromogenic probes, such as 3,3′,5,5′-tetramethylbenzidine (TMB).…”

mentioning

confidence: 99%

“…However, washing steps are required after samples are treated for the removal of unbound analytes . In the one-step immunoassay, results are obtained without any washing step after treating the sample. − Recently, the performance of a one-step immunoassay based on switching peptides was reported to be effective without any washing step. , As shown in Figure a, the switching peptides specifically bind to the frame regions (FRs) at the binding pocket of antibodies (IgGs), and these peptides are released when antigens specifically bind to the complementarity-determining regions (CDRs) of antibodies. As shown in Figure b, the L1 (and L2)-region at the light chain of IgG ( V L ) was known to make self-assembly with the H2 (and H1)-region at the heavy chain of IgG ( V H ) .…”

mentioning

confidence: 99%

“…Therefore, the switching peptides can be quantitatively released from the antibodies according to the number of bound antigens regardless of the type of antigen. Therefore, the switching peptides labeled with fluorescent dyes were dissociated from IgG as soon as the binding of target analytes (antigens) and the one-step immunoassay without any washing step were possible by the measurement of the fluorescence signal. , In this study, switching peptides were employed in an electrochemical one-step immunoassay instead of with optic-based transducers. − Usually, amperometry-based immunoassays have been used for immunoassays using secondary antibodies labeled with enzymes and chromogenic probes, such as 3,3′,5,5′-tetramethylbenzidine (TMB). , In this case, however, washing is required after each reagent exchange step. For nonlabeled electrochemical measurements without using the secondary antibodies, capacitance measurements have been developed using metal electrodes with self-assembled monolayers , and interdigitated electrodes. , However, a washing step after sample treatment was also essentially required for these types of nonlabeled immunoassays.…”

mentioning

confidence: 99%

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Electrochemical One-Step Immunoassay Based on Switching Peptides and Pyrolyzed Carbon Electrodes

et al. 2022

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Switching peptides were designed to bind reversibly to the binding pocket of antibodies (IgG) by interacting with frame regions (FRs). These peptides can be quantitatively released when antigens bind to IgG. As FRs have conserved amino acid sequences, switching peptides can be used as antibodies for different antigens and different source animals. In this study, an electrochemical one-step immunoassay was conducted using switching peptides labeled with ferrocene for the quantitative measurement of analytes. For the effective amperometry of the switching peptides labeled with ferrocene, a pyrolyzed carbon electrode was prepared by pyrolysis of the parylene-C film. The feasibility of the pyrolyzed carbon electrode for the electrochemical one-step immunoassay was determined by analyzing its electrochemical properties, such as its low double-layer capacitance (C dl ), high electron transfer rate (k app ), and wide electrochemical window. In addition, the factors influencing the amperometry of switching peptides labeled with ferrocene were analyzed according to the hydrodynamic radius, the number of intrahydrogen bonds, dipole moments, and diffusion coefficients. Finally, the applicability of the electrochemical one-step immunoassay for the medical diagnosis of the human hepatitis B surface antigen (hHBsAg) was assessed.

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Recent advances in ion‐sensitive field‐effect transistors for biosensing applications

Peng

Mao

et al. 2022

Electrochemical Science Adv

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Over the past decades, considerable development and improvement can be observed in the area of the ion‐sensitive field‐effect transistor (ISFET) for biosensing applications. The mature semiconductor industry provides a solid foundation for the commercialization of the ISFET‐based sensors and extensive research has been conducted to improve the performance of ISFET, with a special research focus on the materials, device structures, and readout topologies. In this review, the basic theories and mechanisms of ISFET are first introduced. Research on ISFET gate materials is reviewed, followed by a summary of typical gate structures and signal readout methods for the ISFET sensing system. After that, a variety of biosensing applications including ions, deoxyribonucleic acid, proteins, and microbes are presented. Finally, the prospects and challenges of the ISFET‐based biosensors are discussed.

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One-step immunoassay without washing steps for influenza A virus detection using ISFET

Cited by 15 publications

References 28 publications

Design and Implementation of a pH Sensor for Micro Solution Based on Nanostructured Ion-Sensitive Field-Effect Transistor

Design and Implementation of a pH Sensor for Micro Solution Based on Nanostructured Ion-Sensitive Field-Effect Transistor

Electrochemical One-Step Immunoassay Based on Switching Peptides and Pyrolyzed Carbon Electrodes

Recent advances in ion‐sensitive field‐effect transistors for biosensing applications

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