One-step immunochromatographic separation and ELISA quantification of glycyrrhizin from traditional Chinese medicines

Recent analytical approaches in quality control of traditional Chinese medicines—A review

“…In addition, Zeng and Xu developed a highly specific and sensitive enzyme-linked immunosorbent assay (ELISA) for the determination of glycyrrhizin (GL) content in TCMs [139,140]. Morinaga described a method to determine saikosaponin content by on-membrane immunoassay, and the result was in agreement with that from the ELISA analysis [141].…”

Study of Separation Conditions of Active Components in Licorice with Two‐Dimensional Liquid Chromatography

“…The typical changes in enthalpy of small molecules are about 10-15 kJ mol 21 , [50] for the four components in licorice their enthalpy changes are about from 0.012 to 32 kJ mol 21 . In the experiment, the 18b-glycyrrhetinic acid and isoliquiritigenin in the SB C 18 column are far away from each other, and their changes in enthalpy are negative, so the raising of temperature is good for the desorption of the two components.…”

Quality Control by Immunoaffinity Concentration Using Monoclonal Antibody

“…Furthermore, we have been establishing several affinity column with MAb against a kind of terpenoid, forskolin (Yanagihara et al, 1996), solasodine glycosides (Putalun et al, 1999), ginsenosides Rb1 (Fukuda et al, 2000c), and GC (Xu et al, 2007). In this chapter, we introduce the new strategy to isolate and concentrate the target bioactive compounds from the crude extract by immunoaffinity column using individual MAb.…”