One‐Step In Situ Biosynthesis of Graphene Oxide–Bacterial Cellulose Nanocomposite Hydrogels

Abstract

Graphene oxide-bacterial cellulose (GO/BC) nanocomposite hydrogels with well-dispersed GO in the network of BC are successfully developed using a facile one-step in situ biosynthesis by adding GO suspension into the culture medium of BC. During the biosynthesis process, the crystallinity index of BC decreases and GO is partially reduced. The experimental results indicate that GO nanosheets are uniformly dispersed and well-bound to the BC matrix and that the 3D porous structure of BC is sustained. This is responsible for efficient load transfer between the GO reinforcement and BC matrix. Compared with the pure BC, the tensile strength and Young's modulus of the GO/BC nanocomposite hydrogel containing 0.48 wt% GO are significantly improved by about 38 and 120%, respectively. The GO/BC nanocomposite hydrogels are promising as a new material for tissue engineering scaffolds.

Submicrofiber‐Incorporated 3D Bacterial Cellulose Nanofibrous Scaffolds with Enhanced Cell Performance

“…Crystalline structure was analyzed by XRD measurement, and the results are presented in Figure a. Similar to our previous studies, BC shows three obvious peaks at 14.2, 16.6, and 22.8°, which results from the diffractions of the (), (110), and (200) planes of cellulose I . The calculated crystallinity index ( C I ) of BC is 84.4%, which is comparable to previously reported values .…”

“…Prior to culturing, the medium was sterilized at 121 °C for 30 min. The harvested hydrogel was purified using the previously reported procedure, providing BC hydrogel. The hydrogel was freeze‐dried, producing BC fabric.…”

Novel porous graphene oxide and hydroxyapatite nanosheets-reinforced sodium alginate hybrid nanocomposites for medical applications

“…ACCEPTED MANUSCRIPT 15 proliferation [17,51]. In addition, adding GO into HAp/SA composites does not cause significant change in cell viability.…”

In situ processing of cellulose nanocomposites

“…Graphene oxide (GO)/BC nanocomposites were synthesised by Si et al [48] using environmentally friendly one step in situ biosynthesis process. A commercially available aqueous dispersion of GO was added to the culture medium and BC was produced from a bacterial strain, A. xylinum X-2.…”