One‐Step Noncovalent Surface Functionalization of PDMS with Chitosan‐Based Bioparticles and Their Protein‐Repellent Properties

Abstract

Stable hydrophilic and protein‐repellent surfaces from nontoxic polysaccharides are highly desirable in several biomedical applications such as implants and prosthetics. Herein, a water‐based one‐step strategy to functionalize polydimethylsiloxane (PDMS) surfaces with chitosan‐based bioparticles is described to suppress the adsorption of blood plasma proteins, which causes biofilm formation and implant‐associated infection. Micro‐ and nanosized chitosan (Chi) and carboxymethyl chitosan (CMChi) particles, featuring high stability, hydrophilicity, and zwitterionic feature, are synthesized in an ecofriendly solvent water and their interaction with PDMS surfaces is investigated in‐real time over a wide range of pH (3–10) using a quartz crystal microbalance with dissipation (QCM‐D). The results show that both Chi and CMChi form stable colloidal particles at pH close to their zero‐point charge, and thus exhibit maximum adsorption and irreversibly bound layers on PDMS surfaces. Coating from CMChi shows high stability, improved hydrophilicity, and subsequently no adsorption of bovine serum albumin and enhanced resistance to fibrinogen, compared to Chi and native PDMS, as revealed by water contact angle and QCM‐D measurements. The results demonstrate the possibility to develop novel and biobased coating materials to address unspecific protein adsorption not only on silicone‐based implants but also on other biomaterials in general.

Influence of Charge and Heat on the Mechanical Properties of Scaffolds from Ionic Complexation of Chitosan and Carboxymethyl Cellulose

“…Figure 5 A,B shows the charge/mass ( Q / m , pH) isotherms. Only one slope was observed for CS 100 and CS 0 ( Figure 5 A,B), with p K a values of 6.8 and 3.5 and total charges of 4.4 ± 0.4 and 3.2 ± 0.3 mmol g –1 , 11 , 54 , 55 respectively. On the contrary, CS 50 / N (A) and CS 40 / N (B) exhibited two slopes, which can be characterized by the difference in Q / m of two distinct plateaus.…”

Bioactive Functional Nanolayers of Chitosan–Lysine Surfactant with Single- and Mixed-Protein-Repellent and Antibiofilm Properties for Medical Implants

“…This shows that the adsorbed nanolayers substantially reduced the hydrophobicity of PDMS due to the introduction of polar and charged groups. 14 , 15 , 31 However, the obtained SCA(H 2 O) of the two functionalized samples are on the edge of hydrophobicity and appear more hydrophilic.…”

“… 43 In this study, we first investigated the interaction and unspecific protein adsorption nature of “model” PDMS surfaces coated with Chi-77KS and Chi-77KS/AMOX using BSA as a generally accepted marker, and whose adsorption is, in principle, driven mainly by hydrophobic interactions with the surface. 14 , 15 , 35 Albumins are a group of proteins, occurring in the body fluids and tissues of mammals. Serum albumin is the most abundant protein in blood.…”

“… 13 Polyelectrolyte–surfactant complexes (PESCs) play a vital role in the pharmaceutical, cosmetic, and food industries. 14 , 15 It was already reported that the use of natural or modified PSs such as Chi, hyaluronic acid, and alginate enables flexible design of bioactive coatings with improved antibacterial and protein-repelling properties to protect surfaces from infections. 16 , 17 However, the studies combining the PSs and surfactants as a bioactive coating toward biofilm formation are scarce.…”

Study on Functionality and Surface Modification of a Stair-Step Liquid-Triggered Valve for On-Chip Flow Control

“…Furthermore, the efficiency of the surface coverage has an impact on the stability of the surface wettability [ 29 ]. As immobilization on planar surfaces yields a limited anchoring point density, the appropriate concentration of functional groups should be employed so as to increase the protein capture capability, resulting in improved immunoassay sensitivity or enzyme conversion rates in immunosensors, for example [ 30 , 31 , 32 , 33 ]. Based on the different purposes of microfluidic devices, especially those for diagnostic applications, there are the considerations of whether it needs to lower the non-specific protein absorption or provide anchor points for antibody grafting.…”